Nutmeg's (Myristica Fragrans Houtt) Oleoresin: Effect of Heating to Chemical Compositions and Antifungal Properties

Utilization of Nutmeg (Myristica fragrans Houtt.) Seed Hydrodistillation Time to Produce Essential Oil Fractions with Varied Compositions and Pharmacological Effects

Molecules ◽

10.3390/molecules25030565 ◽

2020 ◽

Vol 25 (3) ◽

pp. 565 ◽

Cited By ~ 1

Author(s):

Mohamed A. Ibrahim ◽

Charles L. Cantrell ◽

Ekaterina A. Jeliazkova ◽

Tess Astatkie ◽

Valtcho D. Zheljazkov

Keyword(s):

Plasmodium Falciparum ◽

Essential Oil ◽

Seed Oil ◽

Antimalarial Activity ◽

Chemical Compositions ◽

Pharmacological Effects ◽

Substantial Variation ◽

Myristica Fragrans ◽

Oil Fractions ◽

Myristica Fragrans Houtt

The intent of this study was to utilize distillation timeframes (DT) of nutmeg (Myristica fragrans) essential oil (EO) to generate fractions with differential chemical compositions and bioactivity. Ten fractions were captured at the following distillation timeframes: 0.0–0.5, 0.5–1.0, 1.0–2.5, 2.5–5.0, 5.0–10, 10–30, 30–60, 60–90, 90–120, and 120–240 min. In addition, a control EO was collected from a straight 0–240 min non-stop distillation. ANOVA and advanced regression modeling revealed that the produced EO fractions possess substantial variation in the concentration of potentially desired compounds. The concentrations (%) of α-phellandrene, 3-carene, p-cymene, limonene, α-thujene, α-pinene, camphene, sabinene, β-pinene, and myrcene decreased, while the concentrations (%) of α-terpinene, γ-terpinene, terpinolene, and myristicin increased in later DT fractions. Nutmeg EO showed some antimalarial activity against Plasmodium falciparum D6, but did not exhibit significant antifungal activity. In general, nutmeg seed oil yields increased with an increase of DT. These results may be utilized by industries using nutmeg EO.

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ISOLATION OF ESSENSIAL OIL OF NUTMEG (Myristica fragrans Houtt) and ANTIOXIDANT ACTIVITY TEST WITH DPPH

Jurnal Natural ◽

10.24815/jn.v18i1.6604 ◽

2018 ◽

Vol 18 (1) ◽

pp. 11-17 ◽

Cited By ~ 1

Author(s):

Binawati Ginting ◽

Ratna Maira ◽

Mustanir . ◽

Hira Helwati ◽

Lydia Septa Desiyana ◽

...

Keyword(s):

Antioxidant Activity ◽

Essential Oil ◽

Essential Oils ◽

Flavonoid Compound ◽

Piper Nigrum ◽

Chemical Compositions ◽

Green Pepper ◽

Myristica Fragrans ◽

Phenolic Constituents ◽

Myristica Fragrans Houtt

The essential oil of the nutmeg plant (Myristica fragrans Houtt) has been obtained from roots, bark, fruit, mace and seeds using Stahl steam distillation. Each essential oil have tested for antioxidant activity with DPPH. Antioxidant activity of essential oil from each nutmeg plant to DPPH with concentration 25, 50 and 100 ppm. Each essential oil did not show strong antioxidant activity but the result of nutmeg isolation had strong antioxidant activity with IC50 that was 80,555 ppm. Based on GC-MS analysis of essential oil of nutmeg isolate obtained myristicin compound with 96.52% area and time Retention 22.127. Myristicin is one of the main components of essential oils of nutmeg plants that play an important role as antioxidants. Keywords: Nutmeg plant (Myristica fragrans Houtt), Essential Oil, DPPH, Antioxidant radicalsREFERENCES Suryanti, Siti. 2014. Metabolit Sekunder Pada Tanaman dan Fungsinya. http://seputarduniasains.blogspot.com Diakses tanggal 2 juni 2015. Gupta, A. D., Bansal, V. K., Babu, V., Maithil, N. 2013. Chemistry, antioxidant and antimicrobial potential of nutmeg (Myristica fregrans Houtt) Journal of Genetic Engineering and Biotechnologyh 11:25-31. Rodianawati, I., Hastuti, P., Cahyanto, M. N. 2015. Nutmeg’s (Myristica fragrans Houtt) Oleoresin: Effect of Heating to Chemical Compositions and Antifungal Properties Procedia Food Science 3:244-254. Masyithah, Z. 2006. Pengaruh Volume dan Konsentrasi Pelarut pada Isolasi Trimiristin dari Limbah Buah Pala Jurnal Teknologi Proses 5(1) Januari; 64-67. Wibowo, S dan Komarayati, S. 2015. Sifat Fisiko Kimia Minyak Cupresus (Cupressus benthamii) Asal Aek Nauli, Parapat Sumatera Utara Jurnal Penelitian Hasil Hutan 33 (2) Juni 2015: 93-103. Nurdjannah, N. 2007. Teknologi Pengolahan Pala. Badan Penelitian dan Pengembangan Pertanian. Bogor. Hellen, M, Vargheese, T.N, Kumari, J, Abiramy, Sajina, Sree, J, 2012, Phytochemical Analysis and Anticancer Activity of Essential Oil From Myristica fragrans, International Journal of Current Pharmaceutical Review and Research. Chatterjee, S, Zareena Niaz, S. Gautam, Soumyakanti Adhikari, Prasad S. Variyar, Arun Sharma, 2007, Antioxidant Activity of Some Phenolic Constituents from Green Pepper (Piper nigrum L.) and Fresh Nutmeg Mace (Myristica fragrans) J. Food Chemistry 101, 515–523. Sulaiman, S. F and Kheng, L. O. 2012. Antioxidant and anti food-borne bacterial activities of extracts from leaf and different fruit parts of Myristica fragrans Houtt. Food Control 25:533-536.Akinboro, A, Kamaruzzaman, M. B, Asmawi, M. Z, Sulaiman, S. F, Sofiman, O. H. 2011. Antioxidans in Aqueous extract of Mirystica fragrans (Houtt) Suppress Mitosis and Cyclophosphamide-induce chromosomal aberrations in Allium cepa L. Cells, Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology). ISSN 1673-1581 (Print); ISSN 1862-1783 (Online).www.zju.edu.cn/jzus; www.springerlink.comGinting, B., 2013, Aktifitas Antifungi Ektrak Daun Pala (Mirystica fragrans Houtt), Prosiding Seminar Nasional Kimia tahun 2013. Ginting, B, T. Barus, P, Simanjuntak, L. marpaung., 2013, Isolasi dan Sifat Antioksidan Total Flavonoid Daun Pala (Mirystica fragrans Houtt), Prosiding Seminar Nasional Kimia, Samarinda Ginting, B, T. Barus, P, Simanjuntak, L. marpaung.,2013, Isolasi dan Dan Penentuan Aktivitas Antioksidan Total Alkaloid Daun Pala (Mirystica fragrans Houtt), Prosiding Seminar Nasional Yusuf Banseh Ginting, B, T. Barus, P, Simanjuntak, L. marpaung., 2016, Isolation and Identification of Flavonoid Compound from Nutmeg Leaves (Mirystica fragrans Houtt), Asian Journal Of Chemistry. Ginting, B., Mustanir., Helwati, H., Desiyana, L. S., Eralisa, Mujahid, R. 2017. Antioxidant Activity Of N-Hexane Extract Of Nutmeg Plants From South Aceh Province. Jurnal Natural 17(1) Ramaswany, V. N., Varghese, A., Simon. 2011. An Investigation on Cytotoxic Ternatea L International Journal of Drug Discovery 3: 74-77. ISSN : 0975-4423. Guenther, E. 1987. Minyak Atsiri Jilid 1, terjemahan dari: Essensial oil. Penerjemah: Ketaren S, Universitas Indonesia Press, Jakarta.Harborne, J.B. 1987. Metode Fitokimia: Penentuan Cara Moderen Menganalisa Tumbuhan. Terjemahan dari Phytochemical Methods oleh Kosasih Padmawinata. ITB. Bandung.Sipahelut, S. G. 2012. Karakteristik Minyak Daging Buah Pala (Myristica fragrans Houtt) Melalui Beberapa Cara Pengeringan Dan Distilasi Journal Agroforestri 7(1) Maret 2012.Andini, V., Gupta, S., Chatterejee, S., Variyar, P.S and Sharma, A. 2015. Activity Guided Characterization of Antioxidant Components from Essential Oil of Nutmeg (Myristica fragrans). Vol 52; 221-230.Nagja, T., Vimal, K, Sanjeev, A. 2015. Myristica fragrans: A Comprehensive Review International Journal of Pharmacy and Pharmaceutical Sciences 8 (2).Isnindar., Wahyuono, S., Setyowati, E.P. 2011. Isolasi dan Identifikasi Senyawa Antioksidan Daun Kesemek (Diospyros kaki Thunb.) dengan Metode DPPH (2,2-difenil-1-pikrilhidrazil). Majalah Obat Tradisional. 16(3), 157-164.Ramy, M., Fayed, S.A and Mahmoud, G.I. 2010. Chemical Compositions, Antiviral and Activities of Seven Essential Oils. Journal of Applied Sciences Research. 6(1); 50-62.Indriaty, F., Assah, Y., Mamahani, S.N. 2015. Serbuk minuman berbasis daging buah pala. Baristand. Manado.Morita, T., Jinni, K., Kawagishi, H., Arimoto, Y., Suganuma, H., Inakuma, T, and Sigiyama, K. 2003. Hepatoprotective Effect or Myristicin from Nutmeg (Myristica fragrans) on Lipopolisaccaride/d-galactosamine-induced Liver Injury. J. Agric. Food Chem. 15(6):1.560-1.565.Syarifuddin, I., Kaimudin, M., Torry, R.F., dan Biantoro, R. 2014. 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Phytochemical and Pharmacological Activity of Myristica fragrans Houtt (Myristicaceae)

INTERNATIONAL JOURNAL OF TOXICOLOGICAL AND PHARMACOLOGICAL RESEARCH ◽

10.25258/ijtpr.v9i01.9038 ◽

2017 ◽

Vol 9 (01) ◽

Author(s):

Md Arifur Rahman Chowdhury ◽

Manirujjaman . ◽

Md Mazedul Haq

Keyword(s):

Acetic Acid ◽

Phenolic Content ◽

Methanolic Extract ◽

Radical Scavenging ◽

Antioxidant Property ◽

Free Radical Scavenging ◽

Total Phenolic ◽

Writhing Test ◽

Myristica Fragrans ◽

Myristica Fragrans Houtt

Objective: Myristica fragrans Houtt commonly used as traditional medicine for alleviating of various disorders. The purpose of our study was to map out the in vitro antioxidant property and in vivo anti-hyperglycemic and analgesic effect of the methanolic extract of Myristica fragrans Houtt. (Seed and mace) (Myristicaceae) on Swiss albino mice. Methods: The processed powder of Myristica fragrans Houtt (seed and mace) were subjected to methanolic extraction by soxhlet filtration methods, and the desiccated extract was used for screening of antioxidant by DPPH free radical scavenging assessment as well as total phenolic content by using folin-ciocalteu reagent.Anti-hyperglycemic effect and analgesic action tested through alloxan induced antidiabetics test and acetic acid-tempted writhing test on mice. Results: In DPPH free radical scavenging assessment, free radicals neutralization expressed as % of inhibition 49.69±0.06% also by IC50 values as 68.43 µg/ml surmise middle level of antioxidant property. The total phenolic content expressed as 186.25 mg/g equivalent of gallic acid indicates, active phenolic content. Oral administration of 200 and 400 mg/kg of extract dose and reference drug vildagliptin (50 mg/kg) for the duration of the 4-day study period, and initiated % of inhibition the blood glucose level measured as 22.48%, 44.78% and 62.02% regard as the significant anti-hyperglycemic properties. The analgesic activity was investigated by using the acetic acid-induced writhing test in mice, at the dose of 200 mg/kg body and 400mg/kg weight, and resulting 50.4% and 68.10% correspondingly, which was considerably significant with a standard drug. Conclusion: The present study suggests that methanolic extract of seed and mace of Myristica fragrans Houtt can manage moderate oxidative stress as well as perform the painkilling action. Besides, prolong medication may enhance the new dimension of anti-hyperglycemic activity.

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Karekteristik Mikrokapsul Oleoresin Fuli Pala (Myristica Fragrans Houtt) Yang Dienkapsulasi Dengan Gum Arab

Jurnal Teknologi Pangan dan Hasil Pertanian ◽

10.26623/jtphp.v14i2.1281 ◽

2020 ◽

Vol 14 (2) ◽

pp. 1

Author(s):

Alston Millan

Keyword(s):

Scavenging Activity ◽

Myristica Fragrans ◽

Myristica Fragrans Houtt

ABSTRAK Tujuan penelitian ini adalah untuk mengetahui pengaruh formulasi berbagai total padatan yang terdiri dari gum Arab sebagai enkapsulan dan oleoresin fuli pala sebagai core terhadap karakteristik mikrokapsul yang dihasilkan. Variasi formulasi oleoresin fuli pala dan gum arab yang dicobakan adalah A1 (5%), A2 (10%), A3 (15%), A4 (20%) dan A5 (20%). Pembentukan emulsi dilakukan dengan Turrax homogenizer dengan kecepatan 4000 rpm selama 5 menit. Pembentukan mikrokapsul dilakukan dengan menggunakan pengering semprot (spray dryer) dengan suhu inlet dan outlet masing-masing 1100C dan 620C. Mikrokapsul yang dihasilkan dianalisis karakteristiknya yang meliputi rendemen mikrokapsul, kadar oleoresin terkapsulkan, kadar oleoresin tak terkapsulkan, kadar air, engel of repose, wettability dan rehidrasi, aktivitas antioksidan dengan metode RSA (radikal scavenging activity) DPPH (2-2Dhpypenil-2 Picrylhydrazil) dan kromatografi gas-spektrometri massa (GC-MS). Mikrokapsul yang terbaik diperoleh dengan variasi formulasi oleoresin fuli pala dan gum arab adalah A1 (5%). Sifat mikrokapsul yang diperoleh menpunyai rendemen mikrokapsul 24,68%, oleoresin terkapsulkan 92,68%, oleoresin tak terkapsulkan 7,607%, kadar air 8,444%, angel of repose 19,083 (0), wettability 5,9 (menit), aktivitas antioksidan (IC50) 1032 ppm dan kromatografi gas-spektrometri massa (GC-MS) dengan komponen terbesar penyusun mikrokapsul oleoresin fuli pala yaitu sabinene hexane, 4-methylene-1-, myristycin, phenol 2,6 dimetoksi-4-2 (2-peopenil), phenol, 2-metoksi-4-(propennyl).

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