scholarly journals In Vitro Selection of Jatropha Curcas Linn. Hybrids Using Polyethylene Glycol to Obtain Drought Tolerance Character

2015 ◽  
Vol 14 ◽  
pp. 239-245
Author(s):  
Maftuchah ◽  
Agus Zainudin
Keyword(s):  
Polyethylene Glycol ◽  
Drought Tolerance ◽  
Jatropha Curcas ◽  
In Vitro Selection ◽  
Selection Of

In vitro selection of rhizobacteria for increasing drought tolerance of tomato plants

2021 ◽  
pp. 429-436
Author(s):  
A. Gul ◽  
H. Ozaktan ◽  
L. Yolageldi ◽  
H. Altunlu ◽  
G. Aydoner Coban ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
In Vitro Selection ◽  
Tomato Plants ◽  
Selection Of

In vitro selection of Argan tree for drought tolerance and its micropropagation through direct organogenesis

2012 ◽  
Vol 29 ◽  
pp. S184-S185
Author(s):  
Mouna Lamaoui ◽  
Aissam Salama ◽  
Abdelghani Chakhchar ◽  
Abderrahim Ferradous ◽  
Said Wahbi ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
In Vitro Selection ◽  
Direct Organogenesis ◽  
Argan Tree ◽  
Selection Of

Somaclonal Variation in Rice ̵2 Drought Tolerance and Other Agronomic Characters

1995 ◽  
Vol 43 (2) ◽  
pp. 201 ◽  
Author(s):  
SW Adkins ◽  
R Kunanuvatchaidach ◽  
ID Godwin
Keyword(s):  
Polyethylene Glycol ◽  
Drought Tolerance ◽  
Somaclonal Variation ◽  
Environmental Conditions ◽  
In Vitro Selection ◽  
Dry Weight ◽  
Rice Seed ◽  
Agronomic Characters ◽  
Selection Step

The ability to withstand drought was studied in R2 families raised by self-pollination from 149 R1 plants regenerated from callus of mature rice seed (cvs FR13A, Kurkaruppan, Khao Dawk Mali 105, Nam Sagui 19, and Calrose 76). Compared to parental controls, significant improvement in drought tolerance as assessed by dry weight production, was found in 6% of the cv. FR13A families, but not in any of Kurkaruppan or Khao Dawk Mali 105 families tested in a controlled-temperature glasshouse. When a similar study was undertaken using an in vitro selection step (using polyethylene glycol), significant improvement in drought tolerance was found in 6% of Khao Dawk Mali 105 families, but not in any of the Nam Sagui 19 families. No improvements were noted in this latter study when in vitro selection was not used. Variation in other agronomically important characters was observed in the R2 families grown under similar, but well-watered, environmental conditions.

The Effects of Shock Vancomycin Concentrations on the Formation of Heteroresistance in Staphylococcus aureus

2020 ◽  
Vol 65 (9-10) ◽  
pp. 3-7
Author(s):  
V. V. Gostev ◽  
Yu. V. Sopova ◽  
O. S. Kalinogorskaya ◽  
M. E. Velizhanina ◽  
I. V. Lazareva ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
In Vitro Selection ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
High Concentration ◽  
Short Term ◽  
High Concentrations ◽  
Selection Of ◽  
Selection Of Resistance ◽  
Test Cultures

Glycopeptides are the basis of the treatment of infections caused by MRSA (Methicillin-Resistant Staphylococcus aureus). Previously, it was demonstrated that antibiotic tolerant phenotypes are formed during selection of resistance under the influence of high concentrations of antibiotics. The present study uses a similar in vitro selection model with vancomycin. Clinical isolates of MRSA belonging to genetic lines ST8 and ST239, as well as the MSSA (ATCC29213) strain, were included in the experiment. Test isolates were incubated for five hours in a medium with a high concentration of vancomycin (50 μg/ml). Test cultures were grown on the medium without antibiotic for 18 hours after each exposure. A total of ten exposure cycles were performed. Vancomycin was characterized by bacteriostatic action; the proportion of surviving cells after exposure was 70–100%. After selection, there was a slight increase in the MIC to vancomycin (MIC 2 μg/ml), teicoplanin (MIC 1.5–3 μg/ml) and daptomycin (MIC 0.25–2 μg/ml). According to the results of PAP analysis, all strains showed an increase in the area under curve depending on the concentration of vancomycin after selection, while a heteroresistant phenotype (with PAP/AUC 0.9) was detected in three isolates. All isolates showed walK mutations (T188S, D235N, E261V, V380I, and G223D). Exposure to short-term shock concentrations of vancomycin promotes the formation of heteroresistance in both MRSA and MSSA. Formation of VISA phenotypes is possible during therapy with vancomycin.

Sign in / Sign up

Export Citation Format

Share Document