Metabolomic profiling of Vitis vinifera cell suspension culture elicited with silver nitrate by 1H NMR spectrometry and principal components analysis

2007 ◽  
Vol 42 (2) ◽  
pp. 271-274 ◽  
Author(s):  
Hyung-Kyoon Choi ◽  
Jung-Hye Yoon
Keyword(s):  
Suspension Culture ◽  
Vitis Vinifera ◽  
Silver Nitrate ◽  
Cell Suspension ◽  
Principal Components Analysis ◽  
Cell Suspension Culture ◽  
Principal Components ◽  
Metabolomic Profiling ◽  
Nmr Spectrometry ◽  
Components Analysis

Biotransformation of C13-norisoprenoids and monoterpenes by a cell suspension culture of cv. Gamay (Vitis vinifera)

2009 ◽  
Vol 97 (2) ◽  
pp. 203-213 ◽  
Author(s):  
Sandrine Mathieu ◽  
Jérémie Wirth ◽  
François-Xavier Sauvage ◽  
Jean-Paul Lepoutre ◽  
Raymond Baumes ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Vitis Vinifera ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
A Cell

Effects of different factors on friable callus induction and establishment of cell suspension culture of Hovenia dulcis (Rhamnaceae)

Rodriguésia ◽  
2021 ◽  
Vol 72 ◽  
Author(s):  
Ivan Gonçalves Ribeiro ◽  
Tatiana Carvalho de Castro ◽  
Marsen Garcia Pinto Coelho ◽  
Norma Albarello
Keyword(s):  
Secondary Metabolites ◽  
Suspension Culture ◽  
Silver Nitrate ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Cell Suspension Cultures ◽  
Dry Weight ◽  
Friable Callus ◽  
Weight Analysis ◽  
Hovenia Dulcis

Abstract Medicinal plants are an important therapeutic option for a large share of the world’s population. To establish an in vitro culture system for the production of secondary metabolites from Hovenia dulcis, we studied the effect of auxins, cytokinins, absence of light, and silver nitrate on the development of friable callus. Callus cultures were established for the first time and used to obtain cell suspension cultures. Supplementation with KIN (Kinetin) produced calli with both compact and friable areas, while the addition of TDZ (Thidiazuron) only produced compact callus. The maintenance of cultures in the dark induced a slight enhancement on friability when the auxin PIC (Picloram) was present in the culture medium. The addition of silver nitrate promoted the formation of friable calli. Dry weight analysis showed no significant differences in biomass growth, and, therefore, 2.0 mg.L-1 was considered the most suitable treatment. The presence of silver nitrate was not required for the establishment of cell suspension cultures. Dry weight analysis of cell suspensions showed higher biomass production in the absence of silver nitrate. PIC promoted 100% of cell suspension culture formation in the absence of silver nitrate, and higher biomass production was observed with the lowest concentration (0.625 mg.L-1). No morphological differences were observed among the different concentrations of PIC. Phytochemical screening showed the presence of saponins, flavonoids, flavonols and catechins in the extracts obtained from H. dulcis calli. These results show that the cell cultures herein established are potential sources for the production of H. dulcis secondary metabolites of medicinal interest.

Metabolomic differentiation of deer antlers of various origins by 1H NMR spectrometry and principal components analysis

2006 ◽  
Vol 41 (3) ◽  
pp. 1047-1050 ◽  
Author(s):  
Hyung-Kyoon Choi ◽  
Kyoung-Hee Kim ◽  
Kyoung Heon Kim ◽  
Young-Suk Kim ◽  
Min-Won Lee ◽  
...  
Keyword(s):  
Principal Components Analysis ◽  
1H Nmr ◽  
Principal Components ◽  
Nmr Spectrometry ◽  
Components Analysis
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