Influence of inoculum to substrate ratio on the biochemical methane potential of maize in batch tests

2006 ◽  
Vol 41 (6) ◽  
pp. 1444-1450 ◽  
Author(s):  
F. Raposo ◽  
C.J. Banks ◽  
I. Siegert ◽  
S. Heaven ◽  
R. Borja
Keyword(s):  
Biochemical Methane Potential ◽  
Batch Tests ◽  
Methane Potential ◽  
Substrate Ratio

scholarly journals Measurement of Biochemical Methane Potential of Heterogeneous Solid Substrates: Results of a Two-Phase French Inter-Laboratory Study

Water ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2814 ◽  
Author(s):  
Thierry Ribeiro ◽  
Romain Cresson ◽  
Sébastien Pommier ◽  
Sébastien Preys ◽  
Laura André ◽  
...  
Keyword(s):  
Biochemical Methane Potential ◽  
Two Phase ◽  
Bicarbonate Buffer ◽  
Solid Substrates ◽  
Methane Potential ◽  
Repeatability And Reproducibility ◽  
Substrate Ratio ◽  
Two Phases ◽  
Heterogeneous Solid ◽  
Previous Step

Biochemical methane potential (BMP) is essential to determine the production of methane for various substrates; literature shows important discrepancies for the same substrates. In this paper, a harmonized BMP protocol was developed and tested with two phases of BMP tests carried out by eleven French laboratories. Surprisingly, for the three same solid tested substrates (straw; raw mix and dried-shredded mix of potatoes, maize, beef meat and straw; and mayonnaise), the standard deviations of the repeatability and reproducibility inter-laboratory were not enhanced by the harmonized protocol (average of about 25% depending on the substrate), as compared to a previous step where all laboratories used their own protocols. Moreover, statistical analyses of all the results, after removal of the outliers (about 15% of all observations), did not highlight significant effect of the operational effect on BMP (stirring, automatic or manual gas quantification, use of trace metal, uses a bicarbonate buffer, inoculum to substrate ratio) at least for the tested ranges. On the other hand, the average intra-laboratory repeatability was low, about 7%, whatever the protocol, the substrate and the laboratory. It also appears that drying the SA substrate, which contained proteins, carbohydrates, lipids and fibers, does not impact its BMP.

scholarly journals Effect of the inoculum/substrate ratio on the biochemical methane potential (BMP) of grape marc

2020 ◽  
Vol 15 (5) ◽  
pp. 1
Author(s):  
Kessia Caroline Dantas da Silva ◽  
Miriam Cleide Cavalcante de Amorim ◽  
Renan Santana Galvão ◽  
Yandra Beatriz de Oliveira Gonçalves ◽  
Paula Tereza de Souza e Silva ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Biological Treatment ◽  
Organic Waste ◽  
Biochemical Methane Potential ◽  
Biomass Waste ◽  
Grape Marc ◽  
Nutritive Solution ◽  
Methane Potential ◽  
Industrialization Process ◽  
Substrate Ratio

The grape industrialization process produces large volumes of solid organic waste, with the grape bagasse being the main waste generated in the winemaking process. Anaerobic digestion can be used to treat and dispose of agro-industrial biomass waste. The objective of this study was to evaluate the effect of the inoculum/substrate ratio on the Biochemical Methane Potential (BMP) of grape marc. The experiment was performed in laboratory scale through a system of reactor bottles in batches, removing a set of triplicate flasks for sampling and analysis every 48 hours, with the test lasting 12 days. The reactors contained residue, inoculum and 20% of nutritive solution, maintaining 20% of headspace. The reactors were incubated in an incubator at a mesophilic temperature (35 ± 2°C) and shaken manually every 24 hours. Three different inoculum/substrate (I/S) ratios of 0.75, 1.5 and 3 were used to evaluate the methane yield, organic removals and at the end of degradation the morphology of the bacterial community was evaluated by means of scanning electron microscopy. The I/S 3 ratio provided the best results for loading anaerobic systems, indicating that grape marc presents potential for biological treatment through anaerobic digestion.

Relationship of Substrate and Inoculum on Biochemical Methane Potential for Grass and Pig Manure Co-Digestion

2012 ◽  
Vol 512-515 ◽  
pp. 444-448 ◽  
Author(s):  
Sumeth Dechrugsa ◽  
Sumate Chaiprapat
Keyword(s):  
Methane Production ◽  
Methane Yield ◽  
Pig Manure ◽  
Biochemical Methane Potential ◽  
Production Potential ◽  
Methane Potential ◽  
Methane Production Rate ◽  
Substrate Ratio ◽  
Relationship Of ◽  
Careful Investigation

The effects of substrate mix ratio and inoculum/substrate ratio (ISR) on biochemical methane potential of para-grass (PG) and pig manure (PM) were investigated in batch test that maintained temperature at 35±1 oC and continuously shaked at 120 rpm. Cumulative methane production data at different mix ratios and ISRs were evaluated and fitted with Gompertz equation to derive methane production potential (mL) and maximum methane production rate (mL/d). The maximum and average methane yields at each respective ISR of 1, 2, 3, and 4 were [413.0, 315.5], [539.7, 455.6], [590.3, 472.5], and [593.1, 473.5] mL/gVSadded. Relationship of ISR and PG mix ratio to specific methane yield were expressed in quadratic regression model. The generated response surface showed that methane yield was elevated at higher ISR and higher PG mix ratio. This suggests a careful investigation at different ISR and substrate mix ratios should be performed in order to develop a realistic biochemical methane potential of anaerobic co-digestion.

INFLUENCE OF FREEZING/THAWING AND DRYING/MILLING ON BIOCHEMICAL METHANE POTENTIAL

2016 ◽  
Vol 15 (7) ◽  
pp. 1533-1536
Author(s):  
Jin Mi Triolo ◽  
Sven Gjedde Sommer ◽  
Lene Pedersen
Keyword(s):  
Biochemical Methane Potential ◽  
Methane Potential

scholarly journals Batch Anaerobic Co-Digestion and Biochemical Methane Potential Analysis of Goat Manure and Food Waste

Energies ◽  
2021 ◽  
Vol 14 (7) ◽  
pp. 1952
Author(s):  
Ayobami Orangun ◽  
Harjinder Kaur ◽  
Raghava R. Kommalapati
Keyword(s):  
Food Waste ◽  
Gompertz Model ◽  
Water Displacement ◽  
Biochemical Methane Potential ◽  
Greenhouse Gasses ◽  
Nonlinear Regression Models ◽  
The Us ◽  
Methane Potential ◽  
Goat Manure ◽  
Modified Gompertz Model

The improper management of goat manure from concentrated goat feeding operations and food waste leads to the emission of greenhouse gasses and water pollution in the US. The wastes were collected from the International Goat Research Center and a dining facility at Prairie View A&M University. The biochemical methane potential of these two substrates in mono and co-digestion at varied proportions was determined in triplicates and processes were evaluated using two nonlinear regression models. The experiments were conducted at 36 ± 1 °C with an inoculum to substrate ratio of 2.0. The biomethane was measured by water displacement method (pH 10:30), absorbing carbon dioxide. The cumulative yields in goat manure and food waste mono-digestions were 169.7 and 206.0 mL/gVS, respectively. Among co-digestion, 60% goat manure achieved the highest biomethane yields of 380.5 mL/gVS. The biodegradabilities of 33.5 and 65.7% were observed in goat manure and food waste mono-digestions, while 97.4% were observed in the co-digestion having 60% goat manure. The modified Gompertz model is an excellent fit in simulating the anaerobic digestion of food waste and goat manure substrates. These findings provide useful insights into the co-digestion of these substrates.

scholarly journals Rapid Biochemical Methane Potential Evaluation of Anaerobic Co-Digestion Feedstocks Based on Near Infrared Spectroscopy and Chemometrics

Energies ◽  
2021 ◽  
Vol 14 (5) ◽  
pp. 1460
Author(s):  
Jinming Liu ◽  
Changhao Zeng ◽  
Na Wang ◽  
Jianfei Shi ◽  
Bo Zhang ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Variable Selection ◽  
Root Mean Square Error ◽  
Least Squares ◽  
Mean Square Error ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Mean Square ◽  
Biochemical Methane Potential ◽  
Methane Potential

Biochemical methane potential (BMP) of anaerobic co-digestion (co-AD) feedstocks is an essential basis for optimizing ratios of materials. Given the time-consuming shortage of conventional BMP tests, a rapid estimated method was proposed for BMP of co-AD—with straw and feces as feedstocks—based on near infrared spectroscopy (NIRS) combined with chemometrics. Partial least squares with several variable selection algorithms were used for establishing calibration models. Variable selection methods were constructed by the genetic simulated annealing algorithm (GSA) combined with interval partial least squares (iPLS), synergy iPLS, backward iPLS, and competitive adaptive reweighted sampling (CARS), respectively. By comparing the modeling performances of characteristic wavelengths selected by different algorithms, it was found that the model constructed using 57 characteristic wavelengths selected by CARS-GSA had the best prediction accuracy. For the validation set, the determination coefficient, root mean square error and relative root mean square error of the CARS-GSA model were 0.984, 6.293 and 2.600, respectively. The result shows that the NIRS regression model—constructed with characteristic wavelengths, selected by CARS-GSA—can meet actual detection requirements. Based on a large number of samples collected, the method proposed in this study can realize the rapid and accurate determination of the BMP for co-AD raw materials in biogas engineering.

scholarly journals Biochemical Methane Potential of Cork Boiling Wastewater at Different Inoculum to Substrate Ratios

2021 ◽  
Vol 11 (7) ◽  
pp. 3064
Author(s):  
Roberta Mota-Panizio ◽  
Manuel Jesús Hermoso-Orzáez ◽  
Luis Carmo-Calado ◽  
Gonçalo Lourinho ◽  
Paulo Sérgio Duque de Brito
Keyword(s):  
Anaerobic Digestion ◽  
Biogas Production ◽  
Treatment Plant ◽  
Organic Substrates ◽  
Biochemical Methane Potential ◽  
Volatile Solids ◽  
Working Volume ◽  
Methane Potential ◽  
Bmp Test ◽  
Cork Industry

The present study evaluates the digestion of cork boiling wastewater (CBW) through a biochemical methane potential (BMP) test. BMP assays were carried out with a working volume of 600 mL at a constant mesophilic temperature (35 °C). The experiment bottles contained CBW and inoculum (digested sludge from a wastewater treatment plant (WWTP)), with a ratio of inoculum/substrate (Ino/CBW) of 1:1 and 2:1 on the basis of volatile solids (VSs); the codigestion with food waste (FW) had a ratio of 2/0.7:0.3 (Ino/CBW:FW) and the codigestion with cow manure (CM) had a ratio of 2/0.5:0.5 (Ino/CBW:CM). Biogas and methane production was proportional to the inoculum substrate ratio (ISR) used. BMP tests have proved to be valuable for inferring the adequacy of anaerobic digestion to treat wastewater from the cork industry. The results indicate that the biomethane potential of CBWs for Ino/CBW ratios 1:1 and 2:1 is very low compared to other organic substrates. For the codigestion tests, the test with the Ino/CBW:CM ratio of 2/0.7:0.3 showed better biomethane yields, being in the expected values. This demonstrated that it is possible to perform the anaerobic digestion (AD) of CBW using a cosubstrate to increase biogas production and biomethane and to improve the quality of the final digestate.

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