Use of sugarcane bagasse and grass hydrolysates as carbon sources for xylanase production by Bacillus circulans D1 in submerged fermentation

2005 ◽  
Vol 40 (12) ◽  
pp. 3653-3659 ◽  
Author(s):  
D.A. Bocchini ◽  
O.M.M.F Oliveira ◽  
E. Gomes ◽  
R. Da Silva
Keyword(s):  
Sugarcane Bagasse ◽  
Submerged Fermentation ◽  
Carbon Sources ◽  
Bacillus Circulans ◽  
Xylanase Production

Optimization of xylanase production by Bacillus circulans D1 in submerged fermentation using response surface methodology

2002 ◽  
Vol 38 (5) ◽  
pp. 727-731 ◽  
Author(s):  
D.A Bocchini ◽  
H.F Alves-Prado ◽  
L.C Baida ◽  
I.C Roberto ◽  
E Gomes ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Submerged Fermentation ◽  
Bacillus Circulans ◽  
Xylanase Production

scholarly journals Morphogenesis and Production of Enzymes byPenicillium echinulatumin Response to Different Carbon Sources

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Willian Daniel Hahn Schneider ◽  
Laísa dos Reis ◽  
Marli Camassola ◽  
Aldo José Pinheiro Dillon
Keyword(s):  
Enzymatic Activity ◽  
Sugar Cane ◽  
Filter Paper ◽  
Carbon Sources ◽  
Sugar Cane Bagasse ◽  
Xylanase Production ◽  
Penicillium Echinulatum ◽  
The Media ◽  
Filter Paper Activity ◽  
Second Generation Ethanol

The effect of different carbon sources on morphology and cellulase and xylanase production ofPenicillium echinulatumwas evaluated in this work. Among the six carbon sources studied, cellulose and sugar cane bagasse were the most suitable for the production of filter paper activity, endoglucanases, xylanases, andβ-glucosidases. However, sucrose and glucose showedβ-glucosidase activities similar to those obtained with the insoluble sources. The polyacrylamide gels proved the enzymatic activity, since different standards bands were detected in the media mentioned above. Regarding morphology, it was observed that the mycelium in a dispersed form provided the greatest enzymatic activity, possibly due to greater interaction between the substrate and hyphae. These data are important in understanding the physiology of fungi and could contribute to obtaining enzyme with potential application in the technology of second generation ethanol.

Sugarcane Bagasse Hydrothermal Pretreatment Liquors as Suitable Carbon Sources for Hemicellulase Production by Aspergillus niger

2018 ◽  
Vol 11 (2) ◽  
pp. 316-329 ◽  
Author(s):  
Caio de Oliveira Gorgulho Silva ◽  
José Antonio de Aquino Ribeiro ◽  
Augusto Lopes Souto ◽  
Patrícia Verardi Abdelnur ◽  
Luís Roberto Batista ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Sugarcane Bagasse ◽  
Carbon Sources ◽  
Hydrothermal Pretreatment

scholarly journals Insights into Xylan Degradation and Haloalkaline Adaptation through Whole-Genome Analysis of Alkalitalea saponilacus, an Anaerobic Haloalkaliphilic Bacterium Capable of Secreting Novel Halostable Xylanase

Genes ◽  
2018 ◽  
Vol 10 (1) ◽  
pp. 1 ◽  
Author(s):  
Ziya Liao ◽  
Mark Holtzapple ◽  
Yanchun Yan ◽  
Haisheng Wang ◽  
Jun Li ◽  
...  
Keyword(s):  
Single Molecule ◽  
Genomic Sequence ◽  
Carbon Sources ◽  
Compatible Solutes ◽  
Tween 20 ◽  
Sequence Information ◽  
Ph Homeostasis ◽  
Whole Genome Analysis ◽  
Xylanase Production ◽  
Xylan Degradation

The obligately anaerobic haloalkaliphilic bacterium Alkalitalea saponilacus can use xylan as the sole carbon source and produce propionate as the main fermentation product. Using mixed carbon sources of 0.4% (w/v) sucrose and 0.1% (w/v) birch xylan, xylanase production from A. saponilacus was 3.2-fold greater than that of individual carbon sources of 0.5% (w/v) sucrose or 0.5% (w/v) birch xylan. The xylanse is halostable and exhibits optimal activity over a broad salt concentration (2–6% NaCl). Its activity increased approximately 1.16-fold by adding 0.2% (v/v) Tween 20. To understand the potential genetic mechanisms of xylan degradation and molecular adaptation to saline-alkali extremes, the complete genome sequence of A. saponilacus was performed with the pacBio single-molecule real-time (SMRT) and Illumina Misseq platforms. The genome contained one chromosome with a total size of 4,775,573 bps, and a G+C genomic content of 39.27%. Ten genes relating to the pathway for complete xylan degradation were systematically identified. Furthermore, various genes were predicted to be involved in isosmotic cytoplasm via the “compatible-solutes strategy” and cytoplasmic pH homeostasis though the “influx of hydrogen ions”. The halostable xylanase from A. saponilacus and its genomic sequence information provide some insight for potential applications in industry under double extreme conditions.

scholarly journals Xylanase Production in Submerged Fermentation by T. Longibractium MTCC-936

2006 ◽  
Vol 5 (2) ◽  
pp. 26-38
Author(s):  
Ranjeet Singh ◽  
S. K. Mandal
Keyword(s):  
Submerged Fermentation ◽  
Nitrogen Sources ◽  
Salt Precipitation ◽  
Xylanase Production ◽  
Optimum Yield ◽  
Ultra Filtration ◽  
Filtration Technique

Two thermophilic strains of fungi T.longibroctium MTCC 936 andT. harzimum MTCC 792 were examined for their ability to produceXylanose in submerged fermentations. The higher enzyme odivity 27.02 was obtained in submerged fermentation by T.longibroctium. The optimum yield of xylonase was obtained with xylan as a substrate along with combination of three nitrogen sources (Urea,(NH4)2SO4 and Bodopeptone 0.1% each) of pH 6 and temperature 55°C. This enzyme was purified by salt precipitation and ultra filtration technique.

scholarly journals The Carbon Source Controls the Secretion and Yield of Polysaccharide-Hydrolyzing Enzymes of Basidiomycetes

2021 ◽  
Author(s):  
Eka Metreveli ◽  
Tamar Khardziani ◽  
Vladimir Elisashvili
Keyword(s):  
Carbon Source ◽  
Carbon Sources ◽  
Schizophyllum Commune ◽  
Cellulase Production ◽  
Crystalline Cellulose ◽  
Irpex Lacteus ◽  
Xylanase Production ◽  
Hydrolyzing Enzymes ◽  
Pycnoporus Coccineus ◽  
Polymeric Substrates

In the present study, the polysaccharide-hydrolyzing secretomes of Irpex lacteus BCC104, Pycnoporus coccineus BCC310, and Schizophyllum commune BCC632 were analyzed in submerged fermentation conditions to elucidate the effect of chemically and structurally different carbon sources on the expression of cellulases and xylanase. Among polymeric substrates, crystalline cellulose appeared to be the best carbon source providing the highest endoglucanase, total cellulase, and xylanase activities. Mandarin pomace as a growth substrate for S. commune allowed to achieve comparatively high volumetric activities of all target enzymes while wheat straw induced a significant secretion of cellulase and xylanase activities of I. lacteus and P. coccineus. A synergistic effect on the secretion of cellulases and xylanases by the tested fungi was observed when crystalline cellulose was combined with mandarin pomace. In I. lacteus the cellulase and xylanase production is inducible in the presence of cellulose-rich substrates but is suppressed in the presence of an excess of easily metabolizable carbon source. These enzymes are expressed in a coordinated manner under all conditions studied. It was shown that the substitution of glucose in the inoculum medium with Avicel provides accelerated enzyme production by I. lacteus and higher cellulase and xylanase activities of the fungus. These results add new knowledge to the physiology of basidiomycetes to improve cellulase production.

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