scholarly journals Morphogenesis and Production of Enzymes byPenicillium echinulatumin Response to Different Carbon Sources

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Willian Daniel Hahn Schneider ◽  
Laísa dos Reis ◽  
Marli Camassola ◽  
Aldo José Pinheiro Dillon
Keyword(s):  
Enzymatic Activity ◽  
Sugar Cane ◽  
Filter Paper ◽  
Carbon Sources ◽  
Sugar Cane Bagasse ◽  
Xylanase Production ◽  
Penicillium Echinulatum ◽  
The Media ◽  
Filter Paper Activity ◽  
Second Generation Ethanol

The effect of different carbon sources on morphology and cellulase and xylanase production ofPenicillium echinulatumwas evaluated in this work. Among the six carbon sources studied, cellulose and sugar cane bagasse were the most suitable for the production of filter paper activity, endoglucanases, xylanases, andβ-glucosidases. However, sucrose and glucose showedβ-glucosidase activities similar to those obtained with the insoluble sources. The polyacrylamide gels proved the enzymatic activity, since different standards bands were detected in the media mentioned above. Regarding morphology, it was observed that the mycelium in a dispersed form provided the greatest enzymatic activity, possibly due to greater interaction between the substrate and hyphae. These data are important in understanding the physiology of fungi and could contribute to obtaining enzyme with potential application in the technology of second generation ethanol.

scholarly journals Influence of yeast extract enrichment and Pycnoporus sanguineus inoculum on the dephenolisation of sugar-cane bagasse for production of second-generation ethanol

Fuel ◽  
2020 ◽  
Vol 260 ◽  
pp. 116370
Author(s):  
Enrique González-Bautista ◽  
Enrique Alarcón-Gutierrez ◽  
Nathalie Dupuy ◽  
Isabelle Gaime-Perraud ◽  
Fabio Ziarelli ◽  
...  
Keyword(s):  
Yeast Extract ◽  
Sugar Cane ◽  
Second Generation ◽  
Sugar Cane Bagasse ◽  
Pycnoporus Sanguineus ◽  
Second Generation Ethanol

Breeding of Xylanase-Producing Strains by Ion Implantation and Studying of Fermentation Conditions

2011 ◽  
Vol 233-235 ◽  
pp. 1442-1446
Author(s):  
Shi Chang Li ◽  
Dong Dong Wang ◽  
Zhao Yang Zhu ◽  
Hong Xia Liu
Keyword(s):  
Aspergillus Niger ◽  
Ion Implantation ◽  
Wheat Bran ◽  
Carbon Sources ◽  
Catabolic Repression ◽  
Xylanase Production ◽  
Enzymatic Production ◽  
Fermentation Conditions ◽  
The Media ◽  
Fast Acting

With ion implantation, a high xylanase-producing strainAspergillus nigerBU99 was selected. Different late acting carbon sources (wheat bran, wheat straw, corncob and bagasse) had an effect on enzymatic production. Additionally, it was found that fast acting carbon sources (lactose, galactose, sucrose, glucose and maltose) put into the media affected on xylanase production too. Severe catabolic repression was observed in the media with lactose, but other sugars (galactose, sucrose, glucose and maltose) could increase the production of xylanase compared with the control. The production of xylanase was improved too when corncob flour was hydrolyzed by α-amylase, cellulase or/and glucoamylase. Additionally, it was found that the production of xylanase would be almost higher at 60h when the media contained glucose or were hydrolyzed by enzymes.

scholarly journals Influence of some sugars on xylanase production by Aspergillus awamori in solid state fermentation

2002 ◽  
Vol 45 (4) ◽  
pp. 431-437 ◽  
Author(s):  
Judith Liliana Solórzano Lemos ◽  
Nei Pereira Junior
Keyword(s):  
Solid State ◽  
Carbon Source ◽  
Sugar Cane ◽  
Sugar Cane Bagasse ◽  
Aspergillus Awamori ◽  
Birchwood Xylan ◽  
Xylanase Production ◽  
Low Levels ◽  
Xylosidase Activity

Aspergillus awamori showed high extracellular endoxylanase (100 U/ml) and beta-xylosidase activities (3.5 U/ml) when grown on milled sugar cane bagasse as the principal carbon source without treatment. Partial characterization of xylanases showed that the apparent values of Km were 3.12 ± 0.05 mg/ml for endoxylanase (in birchwood xylan) and 0.45 ± 0.05 mM for beta-xylosidase (in p -nitrophenyl beta-D-xylanopiranoside). Corresponding values of Vmax were 6.63 ± 0.02 and 0.078 ± 0.02 mumol/min. Gradual repression of endoxylanase activity was observed when increasing concentrations of glucose and xylose (1, 2, 4, 6 and 8 g of carbohydrate / 4 g of sugar cane bagasse) were added to production media. In contrast, beta-xylosidase activity was stimulated using low levels of carbohydrates (1 g xylose or glucose/ 4 g of sugar cane bagasse).

scholarly journals The Penicillium echinulatum Secretome on Sugar Cane Bagasse

PLoS ONE ◽  
2012 ◽  
Vol 7 (12) ◽  
pp. e50571 ◽  
Author(s):  
Daniela A. Ribeiro ◽  
Júnio Cota ◽  
Thabata M. Alvarez ◽  
Fernanda Brüchli ◽  
Juliano Bragato ◽  
...  
Keyword(s):  
Sugar Cane ◽  
Sugar Cane Bagasse ◽  
Penicillium Echinulatum
Sign in / Sign up

Export Citation Format

Share Document