Different defense responses and brown rot control in two Prunus persica cultivars to essential oil vapours after storage

2016 ◽  
Vol 119 ◽  
pp. 9-17 ◽  
Author(s):  
Meluci David Cindi ◽  
Puffy Soundy ◽  
Gianfranco Romanazzi ◽  
Dharini Sivakumar
Keyword(s):  
Essential Oil ◽  
Prunus Persica ◽  
Brown Rot ◽  
Defense Responses

scholarly journals Citronella essential oil in the control and activation of coffee plants defense response against rust and brown eye spot

2012 ◽  
Vol 36 (4) ◽  
pp. 383-390 ◽  
Author(s):  
Ricardo Borges Pereira ◽  
Gilvaine Ciavareli Lucas ◽  
Fabiano José Perina ◽  
Pedro Martins Ribeiro Júnior ◽  
Eduardo Alves
Keyword(s):  
Essential Oil ◽  
Induced Defense ◽  
Defense Responses ◽  
Plant Diseases ◽  
Negative Effects ◽  
Significant Control ◽  
Citronella Oil ◽  
Coffee Plants ◽  
Induced Defense Responses ◽  
Brown Eye Spot

The rust and brown eye spot are the main coffee diseases. The losses are due to intense defoliation of plants, which has reduced its production and longevity. The brown eye spot also occurs in fruits, with negative effects on the beverage quality. Some essential oils have presented promising results in the control of plant diseases, as an alternative to the use of fungicides. The objective of this study was to evaluate citronella essential oil in the control of rust and brown eye spot and in the activation of coffee plants defense responses. Twelve-month-old plants were sprayed with citronella oil 1000 µL L-1, acibenzolar-S-methyl 200 mg L-1 and tebuconazole fungicide 200 mg L-1. Plants were inoculated with Hemileia vastatrix and Cercospora coffeicola seven days later. The application was repeated after 30 days. Plants with five months were sprayed with the same treatments to assess the induced defense responses. Citronella oil controlled rust and brown eye spot with efficiencies of 47.2% and 29.7%, respectively, while tebuconazole presented control of 96.5% and 90.5%, respectively. Acibenzolar-S-methyl reduced brown eye spot by 55.9% and showed no significant control of rust. Citronella oil increased peroxidase and chitinase activities in five months coffee plants 336, and 24 and 336 hours after spraying, respectively. Acibenzolar-S-methyl increased peroxidase, chitinase and ββ-1,3-glucanase activities 192, 288 and 336; 24 and; 240 hours after spraying, respectively. The treatments did not increase accumulation of phenols, but a significant increase in lignin was observed in plants sprayed with citronella oil.

scholarly journals First Report of Brown Rot Caused by Monilinia fructicola Affecting Peach Orchards in Slovenia

Plant Disease ◽  
2010 ◽  
Vol 94 (9) ◽  
pp. 1166-1166 ◽  
Author(s):  
A. Munda ◽  
M. Viršček Marn
Keyword(s):  
Prunus Persica ◽  
Brown Rot ◽  
Fruit Rot ◽  
Morphological Identification ◽  
Monilinia Fructicola ◽  
Conidial Suspension ◽  
Stone Fruits ◽  
The European Union ◽  
First Report ◽  
Representative Isolate

Monilinia fructicola, the causal agent of brown rot, is a destructive fungal pathogen that affects mainly stone fruits (Prunoideae). It causes fruit rot, blossom wilt, twig blight, and canker formation and is common in North and South America, Australia, and New Zealand. M. fructicola is listed as a quarantine pathogen in the European Union and was absent from this region until 2001 when it was detected in France. In August 2009, mature peaches (Prunus persica cv. Royal Glory) with brown rot were found in a 5-year-old orchard in Goriška, western Slovenia. Symptoms included fruit lesions and mummified fruits. Lesions were brown, round, rapidly extending, and covered with abundant gray-to-buff conidial tufts. The pathogen was isolated in pure culture and identified based on morphological and molecular characters. Colonies on potato dextrose agar (PDA) incubated at 25°C in darkness had an average daily growth rate of 7.7 mm. They were initially colorless and later they were light gray with black stromatal plates and dense, hazel sporogenous mycelium. Colony margins were even. Sporulation was abundant and usually developed in distinct concentric zones. Limoniform conidia, produced in branched chains, measured 10.1 to 17.7 μm (mean = 12.1 μm) × 6.2 to 8.6 μm (mean = 7.3 μm) on PDA. Germinating conidia produced single germ tubes whose mean length ranged from 251 to 415 μm. Microconidia were abundant, globose, and 3 μm in diameter. Morphological characters resembled those described for M. fructicola (1). Morphological identification was confirmed by amplifying genomic DNA of isolates with M. fructicola species-specific primers (2–4). Sequence of the internal transcribed spacer (ITS) region (spanning ITS1 and ITS 2 plus 5.8 rDNA) of a representative isolate was generated using primers ITS1 and ITS4 and deposited in GenBank (Accession No. GU967379). BLAST analysis of the 516-bp PCR product revealed 100% identity with several sequences deposited for M. fructicola in NCBI GenBank. Pathogenicity was tested by inoculating five mature surface-sterilized peaches with 10 μl of a conidial suspension (104 conidia ml–1) obtained from one representative isolate. Sterile distilled water was used as a control. Peaches were wounded prior to inoculation. After 5 days of incubation at room temperature and 100% relative humidity, typical brown rot symptoms developed around the inoculation point, while controls showed no symptoms. M. fructicola was reisolated from lesion margins. Peach and nectarine orchards in a 5-km radius from the outbreak site were surveyed in September 2009 and M. fructicola was confirmed on mummified fruits from seven orchards. The pathogen was not detected in orchards from other regions of the country, where only the two endemic species M. laxa and M. fructigena were present. To our knowledge, this is the first report of M. fructicola associated with brown rot of stone fruits in Slovenia. References: (1) L. R. Batra. Page 106 in: World Species of Monilinia (Fungi): Their Ecology, Biosystematics and Control. J. Cramer, Berlin, 1991. (2) M.-J. Côté et al. Plant Dis. 88:1219, 2004. (3) K. J. D. Hughes et al. EPPO Bull. 30:507, 2000. (4) R. Ioos and P. Frey. Eur. J. Plant Pathol. 106:373, 2000.

Essential oil extracted from peach (Prunus persica) kernel and its physicochemical and antioxidant properties

LWT ◽  
2011 ◽  
Vol 44 (10) ◽  
pp. 2032-2039 ◽  
Author(s):  
Hao Wu ◽  
John Shi ◽  
Sophia Xue ◽  
Yukio Kakuda ◽  
Dongfeng Wang ◽  
...  
Keyword(s):  
Essential Oil ◽  
Prunus Persica ◽  
Antioxidant Properties

scholarly journals Detection of Latent Monilinia Infections in Nectarine Flowers and Fruit by qPCR

Plant Disease ◽  
2017 ◽  
Vol 101 (6) ◽  
pp. 1002-1008 ◽  
Author(s):  
C. Garcia-Benitez ◽  
P. Melgarejo ◽  
A. De Cal
Keyword(s):  
Prunus Persica ◽  
Brown Rot ◽  
Stone Fruit ◽  
Monilinia Fructicola ◽  
Latent Infections ◽  
Established Method ◽  
Latently Infected ◽  
Dna Amounts

Most stone fruit with a latent brown rot infection caused by Monilinia do not develop visible signs of disease until the arrival of fruit at the markets or the consumer’s homes. The overnight freezing-incubation technique (ONFIT) is a well-established method for detecting latent brown rot infections, but it takes between 7 to 9 days. In this report, we inform on the advantages of applying a qPCR-based method to (i) detect a latent brown rot infection in the blossoms and fruit of nectarine trees (Prunus persica var. nucipersica) and (ii) distinguish between the Monilinia spp. in them. For applying this qPCR-based method, artificial latent infections were established in nectarine flowers and fruit using 10 Monilinia fructicola isolates, 8 M. fructigena isolates, and 10 M. laxa isolates. We detected greater amounts of M. fructicola DNA than M. laxa and M. fructigena DNA in latently infected flowers using qPCR. However, greater DNA amounts of M. laxa than M. fructicola were detected in the mesocarp of latently infected nectarines. We found that the qPCR-based method is more sensitive, reliable, and quicker than ONFIT for detecting a latent brown rot infection, and could be very useful in those countries where Monilinia spp. are classified as quarantine pathogens.

scholarly journals COMPONENT COMPOSITION OF ESSENTIAL OIL PRUNUS PERSICA VAR. NECTARINA GROWING IN UZBEKI-STAN

2020 ◽  
pp. 165-170
Author(s):  
Ra"no Botirovna Karabaeva ◽  
Alidzhan Aminovich Ibragimov ◽  
Otabek Mamadaliyevich Nazarov
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Prunus Persica ◽  
Component Composition ◽  
Raw Material ◽  
Oil Composition ◽  
Pale Yellow ◽  
Yellow Color ◽  
The Republic ◽  
Main Components

The article presents the results of determining the composition of essential oils of Prunus persica var. nectarina varieties "yellow nectarine" – "sariк luchchak (uzb.) ", growing in two regions of the Ferghana region of the Republic of Uzbekistan. The pale yellow essential oil was obtained by hydrodistillation from un-dried fresh leaves of plants. The oil composition was determined by chromatography-mass spectrometry on an Agilent 7890 AGC 6890 N gas chromotograph with a quadrupole mass spectrometer (Agilent 5975C inert MSD) as a detector on an HP-5 MS quartz capillary column. An essential oil of pale yellow color was isolated from the leaves of plants. In the composition of essential oils, 56 and 61 compounds were identified in the first and second samples, respectively, wich is 94.55 and 96.00% of the total components. The dominant components of the first saple are camphor bicyclic monoterpene ketones (24.21%), α-thujone (15.00%) and β-thujone (4.27%), aromatic aldehyde benzaldehyde (18.83%) and isobornoleol bicyclic monoterpene alcohol (6.17%). In the second sample, bicyclic monoterpenic ketones (camphor) (36.67%), α-thujone (21.81%) and β-thujone (7.06%) and bicyclic monoterpene alcohol isobornoleol (9.4%) predominate and monocyclic unsaturated monoterpene α-terpinene (2.18%). In both samples, (+)-2-bornanone (camphor) is predominant. The studied variety Prunus persica var. nectarina can serve as a raw material for the production of essential oil, the main components of which are camphor and isobornoleol.

scholarly journals First Report of the Peach Brown Rot Fungus Monilinia fructicola Resistant to Demethylation Inhibitor Fungicides in New Jersey

Plant Disease ◽  
2010 ◽  
Vol 94 (1) ◽  
pp. 126-126 ◽  
Author(s):  
A. Burnett ◽  
N. Lalancette ◽  
K. McFarland
Keyword(s):  
South Carolina ◽  
New Jersey ◽  
Cold Storage ◽  
Prunus Persica ◽  
Crop Protection ◽  
Brown Rot ◽  
Polymorphism Analysis ◽  
Monilinia Fructicola ◽  
Resistant Strains

Reduced sensitivity and resistance of Monilinia fructicola to demethylation inhibitors (DMIs; fungicide group 3) have been previously found in stone fruit orchards in Georgia, South Carolina, Ohio, and New York (2). Resistance development is a major concern because of the importance of DMIs for brown rot management. Eleven single-spore isolates, originally collected during 2006 from separate commercial peach (Prunus persica) orchards in southern New Jersey, were removed from cold storage (5°C) in early 2008 and examined in vitro for resistance to the DMI propiconazole (Orbit 3.6EC; Syngenta Crop Protection, Inc., Greensboro, NC). After 19 months at 5°C, isolate 7 was inhibited 53.4% in growth on potato dextrose agar (PDA) amended at the discretionary dose of 0.3 μg/ml propiconazole; inhibition of the remaining isolates ranged from 81.4 to 100%. Inhibition values were based on two replications of eight colonies per isolate performed after incubation at 25°C for 4 days. Because of the previously reported relationship between duration of cold storage and propiconazole sensitivity, isolate 7 was tentatively deemed resistant (1). To confirm the in vitro results, isolates were grown at 25°C for 7 days on cellophane over PDA. Genomic DNA was isolated from mycelium with the DNeasy Plant Mini Kit (Qiagen, Inc., Valencia, CA). PCR with primers INS65-F and INS65-R was conducted on a GeneAmp thermal cycler (Applied Biosystems, Inc., Foster City, CA) as described previously to amplify a 65-bp region named ‘Mona’ associated with DMI resistance (2). PCR products were separated via electrophoresis on 0.8% agarose gel. The primers amplified a 376-bp fragment from isolate 7 and a 311-bp fragment from all other isolates, thus indicating the presence of Mona in isolate 7. Restriction fragment length polymorphism analysis using the BsrBI enzyme, specific to a single restriction site within Mona, was conducted on the amplified fragments from all isolates. Electrophoresis results showed digestion of the 376-bp fragment from isolate 7 into 140-bp and 236-bp fragments, thereby confirming the presence of Mona; none of the 311-bp fragments from the remaining isolates were cut by BsrBI. Although economic loss from brown rot has not been reported in New Jersey, these results show that propiconazole-resistant strains have been detected since 2006 and it is most likely that resistant strains of the pathogen are still present in commercial peach orchards. To combat this risk, current brown rot control recommendations are incorporating quinone outside inhibitors (QoIs; fungicide group 11) and carboxamides (fungicide group 7) into control programs as a resistance management strategy. More extensive sampling is planned to ascertain the prevalence and location of resistant strains. References: (1) K. D. Cox et al. Phytopathology 97:448, 2007. (2) C.-X. Luo et al. Plant Dis. 92:1099, 2008.

scholarly journals Is the Tolerance of Commercial Peach Cultivars to Brown Rot Caused by Monilinia laxa Modulated by its Antioxidant Content?

Plants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 589
Author(s):  
Vitus I. Obi ◽  
Joaquín Montenegro ◽  
Juan J. Barriuso ◽  
Fayza Saidani ◽  
Christophe Aubert ◽  
...  
Keyword(s):  
Prunus Persica ◽  
Correlation Coefficients ◽  
Brown Rot ◽  
Total Phenolic ◽  
Fruit Traits ◽  
Total Polyphenols ◽  
Breeding Programs ◽  
Phenolic Contents ◽  
Artificial Fruit ◽  
Peach Breeding

Brown rot, caused by Monilinia spp., provokes pre- and post-harvest damage in peach (Prunus persica (L.) Batsch), which causes an economic impact in the industry. With a view to breeding for increased tolerance to this disease, a screening test based upon artificial fruit inoculation was validated on several parental lines of a peach breeding program during the two-period harvest. In addition, cultivars with different total phenolic contents were included in the two-year study. All physicochemical fruit traits recorded at harvest showed differences among all cultivars. The antioxidant compound content determined using spectrophotometry (to measure ascorbic acid and antioxidant capacity) and UPLC-MS (to measure and identify phenolic compounds) also revealed important differences among all genotypes. The rate of brown rot lesion following fruit inoculation varied widely among cultivars, and it was possible to discriminate between highly and less susceptible cultivars. Cultivars with minimal development of damage were identified as germplasm with the desirable allele combination to increase brown rot tolerance in peach breeding programs. Finally, Pearson’s correlation coefficients (r) between pairs of variables were calculated, searching for any biochemical candidate conferring tolerance. The correlation of phytopathological traits with the antioxidant composition, concerning contents of ascorbic, neochlorogenic, and chlorogenic acids and total polyphenols in fruit, is discussed.

scholarly journals First Report of Peach Brown Rot Caused by Monilinia fructicola in Central and Western China

Plant Disease ◽  
2013 ◽  
Vol 97 (9) ◽  
pp. 1255-1255 ◽  
Author(s):  
L. F. Yin ◽  
S. N. Chen ◽  
N. N. Yuan ◽  
L. X. Zhai ◽  
G. Q. Li ◽  
...  
Keyword(s):  
Prunus Persica ◽  
Direct Sequencing ◽  
Eastern China ◽  
Morphological Characteristics ◽  
Brown Rot ◽  
Its Sequences ◽  
Western China ◽  
Its2 Region ◽  
Monilinia Fructicola ◽  
First Report

Brown rot of peach (Prunus persica) in China has been reported to be caused by at least three Monilinia species (1). In the present study, peaches with symptoms resembling brown rot caused by Monilinia species were collected from commercial orchards in the northwestern province of Gansu in August 2010, the southwestern province of Yunnan in July 2011, and in the central province of Hubei in July 2012. Affected fruit showed the typical symptoms of brown rot with zones of sporulation. Fungal isolates were single-spored and cultured on potato dextrose agar (PDA). Colonies showed grayness with concentric rings of sporulation after incubation at 25°C in the dark. Mean mycelial growth of isolates YHC11-1a and YHC11-2a from Yunnan, GTC10-1a and GTC10-2a from Gansu, and HWC12-14a and HWC12-23a from Hubei, was 4.6 ± 0.4 and 7.5 ± 0.7 cm after 3 and 5 days incubation, respectively. Conidia were lemon shaped and formed in branched monilioid chains, and the mean size was 9.3 (6.7 to 11.5) × 12.5 (7.9 to 17.8) μm, which was consistent with the characteristics of Monilinia fructicola (1,2). The species identification was confirmed by sequencing of the ribosomal ITS sequences. The ribosomal ITS1-5.8S-ITS2 region was amplified from each of the six isolates using primers ITS1 and ITS4 (3). Results indicated that the ITS sequences of these isolates were identical and showed the highest similarity (100%) with M. fructicola ITS sequences from isolates collected in China (GenBank Accession Nos. HQ893748, FJ515894, and AM887528), Slovenia (GU967379), Italy (FJ411109), and Spain (EF207423). The pathogen was also confirmed to be M. fructicola based on the detection of an M. fructicola- specific band (534 bp) using a PCR-based molecular tool developed for distinguishing Chinese Monilinia species affecting peach (1). Pathogenicity was tested on surface-sterilized, mature peaches (Shui Mi Tao) with representative isolates. Fruits were holed at three equidistant positions to a depth of 5 mm using a sterile cork borer. Mycelial plugs (5 mm in diameter) from the periphery of a 4-day-old colony of each isolate were placed upside down into each hole, control fruits received water agar. After 3 days of incubation at 22°C in a moist chamber, inoculated fruits developed typical brown rot symptoms while control fruits remained healthy. Pathogens from the inoculated fruit were confirmed to be M. fructicola based on morphological characteristics. To our knowledge, this is the first report of occurrence of M. fructicola in Gansu, Yunnan, and Hubei provinces, thousands of kilometers away from eastern China where occurrence of peach brown rot caused by M. fructicola has been confirmed (2,4). The results indicated the further geographical spread of the M. fructicola in China. References: (1) M. J. Hu et al. Plos One 6(9):e24990, 2011. (2) M. J. Hu et al. Plant Dis. 95:225, 2011. (3) T. J. White et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Academic Press, San Diego, 1990. (4) X. Q. Zhu et al. Plant Pathol. 54:575, 2005.

Fruit core rot of the peach cultivar 'Fayette' caused by Alternaria alternata in Greece

2007 ◽  
Vol 47 (12) ◽  
pp. 1476 ◽  
Author(s):  
T. Thomidis ◽  
T. J. Michailides ◽  
I. Karayiannis
Keyword(s):  
Alternaria Alternata ◽  
Prunus Persica ◽  
Brown Rot ◽  
Potato Dextrose Agar ◽  
The Other ◽  
Total Production ◽  
Ripening Stage ◽  
Thiophanate Methyl ◽  
Immature Fruit ◽  
The Core

This is the first report of the occurrence of core rot on peaches [Prunus persica (L.) Batsch] caused by the fungus Alternaria alternata in Greece. This disease caused significant preharvest (at commercial ripening stage) and postharvest damage (at a percentage of ~5% of the total production) in the cultivar ‘Fayette’. Rotting of the core began while fruit were still on the tree, with a soft, wet, brown rot progressing in the flesh around the stone. A. alternata was isolated on acidified potato dextrose agar from the edges of the rotted tissues. Symptoms were reproduced under laboratory conditions by injection of spore suspensions in the flesh of mature and immature fruit of ‘Fayette’. In contrast, immature fruit did not show core rot symptoms when naturally infected. Furthermore, none of the other peach cultivars tested (‘Spring Lady’, ‘Spring Crest’, ‘June Gold’, ‘Red Haven’, ‘Sun Crest’, ‘Sun Cloud’) showed the symptoms of core rot of fruit at the commercial ripening stage. The rates of infection were significantly reduced at 10°C and completely inhibited at 2–4°C. The effectiveness of the fungicides PIΛAZIN 60WP, Thiophanate methyl 70WP, Folicur 25WG, Rovral 50WP, Dithane M-45 80WP and Switch 25/37.5 WP, at rates recommended by the manufacturers, were evaluated against A. alternata on agar and artificially inoculated fruit. The fungicides Folicur 25WG, Rovral 50WP and Switch 25/37.5 WP significantly reduced the development of A. alternata. Moderate effectiveness was shown by Dithane M-45 80WP. In contrast, the fungicides PIΛAZIN 60WP and Thiophanate methyl 70WP were not effective against this pathogen.

Estimates of genetic parameters for brown rot resistance in Prunus persica

2021 ◽  
pp. 289-298
Author(s):  
S. Scariotto ◽  
M. Dini ◽  
M.C.B. Raseira ◽  
J. Santos
Keyword(s):  
Genetic Parameters ◽  
Prunus Persica ◽  
Brown Rot
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