Dual crosslinked keratin-alginate fibers formed via ionic complexation of amide networks with improved toughness for assembling into braids

2020 ◽  
Vol 81 ◽  
pp. 106286 ◽  
Author(s):  
Ashmita Mukherjee ◽  
Yogesh H. Kabutare ◽  
Paulomi Ghosh
Keyword(s):  
Ionic Complexation ◽  
Alginate Fibers

Cytotoxicity and Antiviral Activity of Calcium Alginate Fibers and Zinc Alginate Fibers

2010 ◽  
Vol 152-153 ◽  
pp. 1475-1478 ◽  
Author(s):  
Ying Gong ◽  
Guang Ting Han ◽  
Xin Ling Li ◽  
Yan Wu ◽  
Yuan Ming Zhang ◽  
...  
Keyword(s):  
Calcium Alginate ◽  
Activity Assay ◽  
Cytotoxic Effects ◽  
African Green Monkey Kidney ◽  
Relative Growth ◽  
Human Cervical Cancer Cell ◽  
Green Monkey ◽  
Alginate Fibers ◽  
Alginate Fibres ◽  
Mtt Assays

The cytotoxicity and anti-influenza virus (IFV) activity of calcium or zinc alginate fibers were investigated to explore the feasibility of them to be used as biomaterials. African Green Monkey kidney cell (Vero) and human cervical cancer cell (Hela) cultured with alginate fibres were used to screen cytotoxic effects. After 48 h, MTT (3-(4,5-dimethyl-2-thiazol)-2,5-diphenyl-2H tetrazolium bromide) assays were performed. Then cytotoxicity was evaluated with six grades according to cell relative growth rate (RGR). In anti-IFV activity assay, IFV were added to all fibers and the Vero cell survival were detected by MTT assays with calculating the percentage of protection. The cytotoxity of calcium alginate fibers on Vero were grade 0 or 1 in contrast to zinc alginate fibers which was grade 0. The cytotoxity of calcium or zinc alginate fibers on Hela were grade 0. Furthermore, partial calcium or zinc alginate fibers could promote Vero or Hela cell growth. In antiviral assay the highest percentage of protection of calcium alginate fibers was 34.42%, while that of zinc alginate fibers was 59.42%. The results showed that calcium or zinc alginate fibers had a good cellular biocompatibility and the large weight zinc alginate fibers had a better anti-IFV activity than calcium alginate fibers, which is potential for tissue engineering.

Preparation and characterization of poly (ethylene glycol) grafted Ca-alginate fibers by γ-irradiation for biomedical applications

2013 ◽  
Vol 27 (2) ◽  
pp. 216-226 ◽  
Author(s):  
Md. Kamal Khan ◽  
Mohammed Mizanur Rahman ◽  
Bodrun Nesa ◽  
Romana Nasrin ◽  
Swajal Molla ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Biomedical Applications ◽  
Poly Ethylene Glycol ◽  
Γ Irradiation ◽  
Alginate Fibers ◽  
Poly Ethylene ◽  
Ca Alginate

CELL LADEN ALGINATE/ALBUMIN HYDROGEL FIBERS FOR POTENTIAL SKIN TISSUE ENGINEERING APPLICATIONS

2018 ◽  
Vol 30 (06) ◽  
pp. 1850045
Author(s):  
Maria Grazia Cascone ◽  
Elisabetta Rosellini ◽  
Simona Maltinti ◽  
Andrea Baldassare ◽  
Luigi Lazzeri
Keyword(s):  
Tissue Engineering ◽  
A549 Cells ◽  
Average Diameter ◽  
Favorable Effect ◽  
Engineering Applications ◽  
Residual Amount ◽  
Spinning Process ◽  
Proliferation Ability ◽  
Alginate Fibers ◽  
Kinetics Of

Alginate hydrogel fibers are receiving a great attention for tissue engineering applications. However, an important limitation of alginate is that it does not provide cell adhesion motifs. In this work, albumin was blended with alginate to improve the compatibility of alginate fibers with cells. Cell laden alginate/albumin fibers, potentially usable for skin regeneration, were obtained through a spinning process, by extruding an alginate/albumin solution containing cells into a calcium chloride solution. Cell laden pure alginate fibers were prepared for comparison. Plain alginate and alginate/albumin fibers were also produced. Morphological, mechanical and functional properties of the produced fibers were investigated. In addition, the ability of the fibers to release albumin and to support the viability and growth of A549 cells embedded into them was studied. Fibers with a uniform shape and an average diameter within the range 550–570[Formula: see text][Formula: see text]m were produced. The water content was [Formula: see text]% for alginate fibers, and [Formula: see text]% for alginate/albumin fibers. Stress–strain tests showed, up to a strain value of 20%, the same Young’s modulus for the produced fibers, regardless of the presence of albumin. Overall, obtained results demonstrated that morphology, size, hydrophilicity and mechanical properties were not affected by albumin. Albumin was gradually released over a period of 4 days, with a residual amount (13%) remaining into the fibers. Viability test was carried out on A549 cells, laden inside alginate and alginate/albumin fibers, to evaluate cell proliferation ability. A favorable effect of albumin on the loaded cells was evidenced by a faster kinetics of growth.

Fabrication and Application of Polyethylenimine/Ca-Alginate Blended Hydrogel Fibers as High-Capacity Adsorbents for Recovery of Gold from Acidic Solutions

2017 ◽  
Vol 262 ◽  
pp. 103-106 ◽  
Author(s):  
John Kwame Bediako ◽  
Myung Hee Song ◽  
Yeoung Sang Yun
Keyword(s):  
High Capacity ◽  
Metallic Gold ◽  
X Ray Diffraction ◽  
Acidic Solutions ◽  
Xrd Study ◽  
Desorption Study ◽  
Alginate Fibers ◽  
Direct Adsorption ◽  
Ca Alginate ◽  
Alginate Matrix

High-capacity polyethylenimine (PEI)/Ca-alginate blended hydrogel fibers were fabricated via three steps, viz. electrostatic blending of PEI and alginate, ionotropic gelation of alginate and CaCl2, and fixing of PEI into the Ca-alginate matrix, using glutaraldehyde (GA) as a crosslinker. Two crosslinking approaches resulted in different stabilities and gold uptake capacities of the prepared sorbents. Post-crosslinking approach was more efficient than pre-crosslinking likely owing to the better crosslinking efficiency, leading to better stability and sorption capacity. Furthermore, X-ray diffraction (XRD) study revealed the reduction of Au (III) to metallic gold, Au (0) in the crosslinked fibers. The Au (0) predominancy was confirmed with a metal desorption study. The present study thus demonstrates the possibility of recovering metallic gold from aqueous solutions by direct adsorption-coupled-reduction approach using GA-crosslinked PEI/Ca-alginate fibers.

scholarly journals RECIRCULACIÓN DE LÍQUIDOS RESIDUALES EN LA CONVERSIÓN DE ALGINATO DE CALCIO EN ÁCIDO ALGÍNICO DURANTE EL PROCESO DE PRODUCCIÓN DE ALGINATOS

2005 ◽  
Vol 20 (1-2) ◽  
pp. 1
Author(s):  
Y. E. Rodríguez-Montesinos ◽  
G. Hernández-Carmona ◽  
D. L. Arvizu-Higuera
Keyword(s):  
Calcium Alginate ◽  
Alginic Acid ◽  
Current System ◽  
Macrocystis Pyrifera ◽  
Alginate Production ◽  
Yield And Quality ◽  
Alginate Fibers ◽  
Counter Current ◽  
Very High

Se estudió el efecto de recircular la solución ácida residual en la etapa de conversión de alginato de calcio en ácido algínico, utilizando el alga Macrocystis pyrifera . Los líquidos residuales fueron reciclados en un sistema en contra corriente, con lo cual se logró procesar tres cargas de alginato de sodio con el mismo volumen de agua, permitiendo una conversión efectiva en ácido algínico, con una reducción del 56% en el consumo de agua dulce. Se experimentó un sistema de recirculación en línea (sin reemplazo de agua), este sistema no es recomendable, debido a que la acumulación de calcio en el alginato después de la segunda recirculación, produce una viscosidad aparente muy alta, con un porcentaje de reducció superior al 50%. Se determinó el efecto del número de lavados ácidos del ácido algínico sobre la calidad y rendimiento del alginato obtenido. El tratamiento ácido se llevó a cabo con tres, dos y un lavado. Se concluye que se requieren tres lavados de las fibras de alginato de calcio para lograr una conversión efectiva en ácido algínico, pero el primero y segundo lavado se pueden hacer con ácido reciclado. Es tesis tema representa un ahorro del 66% en el consumo de agua en esta etapa. Recycling of residual liquids from the conversion of calcium alginate to alginic acid during alginate production process The effect of recycling the residual acid solution from the conversion of calcium alginate to alginic acid from the alga Macrocystis pyrifera was studied. The residual liquid was recycled using a counter current system; it was possible to treat three batches of calcium alginate with the same amount of water, with an effective conversion into alginic acid, saving 56% of fresh water. An inline recycling system was experimented (without water replacement). This system is not recommended, because the large increase of calcium in the alginate after the second recycling, produces a very high apparent viscosity. Using this system the viscosity was reduced in more than 50%. We experimented the effect of the number of acid washings of the alginic acid, on the yield and quality of the final alginate. The acid treatment was carried out with three, two and one washing. It was concluded that three acid washings of the calcium alginate fibers are necessary to obtain an effective conversion of calcium alginate to alginic acid, but the first and second washings can be carried out with recycled acid. This system represents a water saving up to 66% in this step.

scholarly journals Nanopatterned Protein Films Directed by Ionic Complexation with Water-Soluble Diblock Copolymers

2012 ◽  
Vol 45 (11) ◽  
pp. 4572-4580 ◽  
Author(s):  
Bokyung Kim ◽  
Christopher N. Lam ◽  
Bradley D. Olsen
Keyword(s):  
Diblock Copolymers ◽  
Water Soluble ◽  
Protein Films ◽  
Ionic Complexation

Design for dynamic hydrogen bonding in a double network structure to improve the mechanical properties of sodium alginate fibers

2021 ◽  
Author(s):  
Ming Yan ◽  
Junfeng Shi ◽  
Song Tang ◽  
Guohang Zhou ◽  
Jiexiang Zeng ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Hydrogen Bonding ◽  
Sodium Alginate ◽  
Network Structure ◽  
Wet Spinning ◽  
Double Network ◽  
Alginate Fibers

The SA/PAA-VSNP fiber was obtained using dynamic wet spinning through dynamic hydrogen bonding in the double network structure.

Strengthening and toughening sodium alginate fibers using a dynamically cross-linked network of inorganic nanoparticles and sodium alginate through the hydrogen bonding strategy

2021 ◽  
Author(s):  
Ming Yan ◽  
Junfeng Shi ◽  
Song Tang ◽  
Lingwei Liu ◽  
Haotong Zhu ◽  
...  
Keyword(s):  
Hydrogen Bonding ◽  
Sodium Alginate ◽  
Inorganic Nanoparticles ◽  
Alginate Fibers

Nanoparticles were introduced to strengthen and toughen sodium alginate fibers through a dynamically cross-linked network by hydrogen bonding.

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