Characterization of morphology and mechanical properties of block copolymers using atomic force microscopy: Effects of processing conditions

Polymer ◽  
2012 ◽  
Vol 53 (9) ◽  
pp. 1960-1965 ◽  
Author(s):  
Dong Wang ◽  
Ken Nakajima ◽  
So Fujinami ◽  
Yuji Shibasaki ◽  
Jun-Qiang Wang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Block Copolymers ◽  
Processing Conditions ◽  
Force Microscopy ◽  
Atomic Force ◽  
Morphology And Mechanical Properties

scholarly journals Variation of Burkholderia cenocepacia cell wall morphology and mechanical properties during cystic fibrosis lung infection, assessed by atomic force microscopy

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
A. Amir Hassan ◽  
Miguel V. Vitorino ◽  
Tiago Robalo ◽  
Mário S. Rodrigues ◽  
Isabel Sá-Correia
Keyword(s):  
Mechanical Properties ◽  
Cystic Fibrosis ◽  
Atomic Force Microscopy ◽  
Cell Wall ◽  
Adaptive Evolution ◽  
Burkholderia Cenocepacia ◽  
Force Microscopy ◽  
Atomic Force ◽  
Morphology And Mechanical Properties

Abstract The influence that Burkholderia cenocepacia adaptive evolution during long-term infection in cystic fibrosis (CF) patients has on cell wall morphology and mechanical properties is poorly understood despite their crucial role in cell physiology, persistent infection and pathogenesis. Cell wall morphology and physical properties of three B. cenocepacia isolates collected from a CF patient over a period of 3.5 years were compared using atomic force microscopy (AFM). These serial clonal variants include the first isolate retrieved from the patient and two late isolates obtained after three years of infection and before the patient’s death with cepacia syndrome. A consistent and progressive decrease of cell height and a cell shape evolution during infection, from the typical rods to morphology closer to cocci, were observed. The images of cells grown in biofilms showed an identical cell size reduction pattern. Additionally, the apparent elasticity modulus significantly decreases from the early isolate to the last clonal variant retrieved from the patient but the intermediary highly antibiotic resistant clonal isolate showed the highest elasticity values. Concerning the adhesion of bacteria surface to the AFM tip, the first isolate was found to adhere better than the late isolates whose lipopolysaccharide (LPS) structure loss the O-antigen (OAg) during CF infection. The OAg is known to influence Gram-negative bacteria adhesion and be an important factor in B. cenocepacia adaptation to chronic infection. Results reinforce the concept of the occurrence of phenotypic heterogeneity and adaptive evolution, also at the level of cell size, form, envelope topography and physical properties during long-term infection.

Visco-hyperelastic characterization of the mechanical properties of human fallopian tube tissue using atomic force microscopy

Materialia ◽  
2021 ◽  
Vol 16 ◽  
pp. 101074
Author(s):  
Fereshteh Jafarbeglou ◽  
Mohammad Ali Nazari ◽  
Fatemeh Keikha ◽  
Saeid Amanpour ◽  
Mojtaba Azadi
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Fallopian Tube ◽  
Force Microscopy ◽  
Human Fallopian Tube ◽  
Atomic Force

Investigating the Mechanical Properties of Biological Brain Cells With Atomic Force Microscopy

2018 ◽  
Vol 12 (4) ◽  
Author(s):  
Tariq Mohana Bahwini ◽  
Yongmin Zhong ◽  
Chengfan Gu ◽  
Zeyad Nasa ◽  
Denny Oetomo
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Cancer Cells ◽  
Force Microscopy ◽  
Indentation Force ◽  
Young’S Moduli ◽  
Atomic Force ◽  
Fitting Algorithm ◽  
Cell Mechanical Properties

Characterization of cell mechanical properties plays an important role in disease diagnoses and treatments. This paper uses advanced atomic force microscopy (AFM) to measure the geometrical and mechanical properties of two different human brain normal HNC-2 and cancer U87 MG cells. Based on experimental measurement, it measures the cell deformation and indentation force to characterize cell mechanical properties. A fitting algorithm is developed to generate the force-loading curves from experimental data. An inverse Hertzian method is also established to identify Young's moduli for HNC-2 and U87 MG cells. The results demonstrate that Young's modulus of cancer cells is different from that of normal cells, which can help us to differentiate normal and cancer cells from the biomechanical viewpoint.

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