Quasi-static and hydrodynamic interaction between solid surfaces in polyisoprene studied by atomic force microscopy

Polymer ◽  
2006 ◽  
Vol 47 (20) ◽  
pp. 7259-7270 ◽  
Author(s):  
Rüdiger Stark ◽  
Elmar Bonaccurso ◽  
Michael Kappl ◽  
Hans-Jürgen Butt
Keyword(s):  
Atomic Force Microscopy ◽  
Hydrodynamic Interaction ◽  
Solid Surfaces ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Movements of Mycoplasma mobile gliding machinery detected by high-speed atomic force microscopy

2021 ◽  
Author(s):  
Kohei Kobayashi ◽  
Noriyuki Kodera ◽  
Taishi Kasai ◽  
Yuhei O Tahara ◽  
Takuma Toyonaga ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Internal Structure ◽  
Sodium Azide ◽  
High Speed ◽  
Atp Hydrolysis ◽  
Solid Surfaces ◽  
Force Microscopy ◽  
Special Mechanism ◽  
Atomic Force ◽  
Internal Structures

ABSTRACTMycoplasma mobile, a parasitic bacterium, glides on solid surfaces, such as animal cells and glass by a special mechanism. This process is driven by the force generated through ATP hydrolysis on an internal structure. However, the spatial and temporal behaviors of the internal structures in living cells are unclear. In this study, we detected the movements of the internal structure by scanning cells immobilized on a glass substrate using high-speed atomic force microscopy (HS-AFM). By scanning the surface of a cell, we succeeded in visualizing particles, 2 nm in hight and aligned mostly along the cell axis with a pitch of 31.5 nm, consistent with previously reported features based on electron microscopy. Movements of individual particles were then analyzed by HS-AFM. In the presence of sodium azide, the average speed of particle movements was reduced, suggesting that movement is linked to ATP hydrolysis. Partial inhibition of the reaction by sodium azide enabled us to analyze particle behavior in detail, showing that the particles move 9 nm right, relative to the gliding direction, and 2 nm into the cell interior in 330 ms, then return to their original position, based on ATP hydrolysis.IMPORTANCEThe Mycoplasma genus contains bacteria generally parasitic to animals and plants. Some Mycoplasma species form a protrusion at a pole, bind to solid surfaces, and glide by a special mechanism linked to their infection and survival. The special machinery for gliding can be divided into surface and internal structures that have evolved from rotary motors represented by ATP synthases. This study succeeded in visualizing the real-time movements of the internal structure by scanning from the outside of the cell using an innovative high-speed atomic force microscope, and then analyzing their behaviors.

A study of molecular adsorption of a cationic surfactant on complex surfaces with atomic force microscopy

The Analyst ◽  
2016 ◽  
Vol 141 (3) ◽  
pp. 1017-1026 ◽  
Author(s):  
I. Sokolov ◽  
G. Zorn ◽  
J. M. Nichols
Keyword(s):  
Atomic Force Microscopy ◽  
Cationic Surfactant ◽  
Solid Surfaces ◽  
Molecular Adsorption ◽  
Complex Surfaces ◽  
Force Microscopy ◽  
Atomic Force ◽  
Broad Interest

The study of molecular adsorption on solid surfaces is of broad interest.

Forces Between Solid Surfaces Across Polymer Melts as Revealed by Atomic Force Microscopy

2007 ◽  
Vol 5 (2-3) ◽  
pp. 49-60 ◽  
Author(s):  
Hans-Juergen Butt ◽  
Jijun Wang ◽  
Rüdiger Stark ◽  
Michael Kappl ◽  
Bernhard Anton Wolf ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Polymer Melts ◽  
Solid Surfaces ◽  
Force Microscopy ◽  
Atomic Force

Challenges in imaging of soft layers and structures at solid surfaces using atomic force microscopy

2014 ◽  
Vol 2 (3) ◽  
pp. 151-159 ◽  
Author(s):  
Marta Krasowska ◽  
Anna Niecikowska ◽  
David A. Beattie
Keyword(s):  
Atomic Force Microscopy ◽  
Solid Surfaces ◽  
Force Microscopy ◽  
Atomic Force

Surface charge mapping of solid surfaces in water by pulsed-force-mode atomic force microscopy

1998 ◽  
Vol 66 (7) ◽  
pp. S349-S352 ◽  
Author(s):  
T. Miyatani ◽  
S. Okamoto ◽  
A. Rosa ◽  
O. Marti ◽  
M. Fujihira
Keyword(s):  
Atomic Force Microscopy ◽  
Surface Charge ◽  
Solid Surfaces ◽  
Force Microscopy ◽  
Atomic Force ◽  
Force Mode ◽  
Mode Atomic Force Microscopy

scholarly journals Movements of Mycoplasma mobile Gliding Machinery Detected by High-Speed Atomic Force Microscopy

mBio ◽  
2021 ◽  
Vol 12 (3) ◽  
Author(s):  
Kohei Kobayashi ◽  
Noriyuki Kodera ◽  
Taishi Kasai ◽  
Yuhei O. Tahara ◽  
Takuma Toyonaga ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Internal Structure ◽  
Sodium Azide ◽  
High Speed ◽  
Atp Hydrolysis ◽  
Solid Surfaces ◽  
Force Microscopy ◽  
Special Mechanism ◽  
Atomic Force ◽  
Internal Structures

ABSTRACT Mycoplasma mobile, a parasitic bacterium, glides on solid surfaces, such as animal cells and glass, by a special mechanism. This process is driven by the force generated through ATP hydrolysis on an internal structure. However, the spatial and temporal behaviors of the internal structures in living cells are unclear. In this study, we detected the movements of the internal structure by scanning cells immobilized on a glass substrate using high-speed atomic force microscopy (HS-AFM). By scanning the surface of a cell, we succeeded in visualizing particles, 2 nm in height and aligned mostly along the cell axis with a pitch of 31.5 nm, consistent with previously reported features based on electron microscopy. Movements of individual particles were then analyzed by HS-AFM. In the presence of sodium azide, the average speed of particle movements was reduced, suggesting that movement is linked to ATP hydrolysis. Partial inhibition of the reaction by sodium azide enabled us to analyze particle behavior in detail, showing that the particles move 9 nm right, relative to the gliding direction, and 2 nm into the cell interior in 330 ms and then return to their original position, based on ATP hydrolysis. IMPORTANCE The Mycoplasma genus contains bacteria generally parasitic to animals and plants. Some Mycoplasma species form a protrusion at a pole, bind to solid surfaces, and glide by a special mechanism linked to their infection and survival. The special machinery for gliding can be divided into surface and internal structures that have evolved from rotary motors represented by ATP synthases. This study succeeded in visualizing the real-time movements of the internal structure by scanning from the outside of the cell using an innovative high-speed atomic force microscope and then analyzing their behaviors.

Microstructures in Lubricant Thin Layers at the Magnetic Disk Surface, Observed Using Cryogenic Atomic Force Microscopy

2006 ◽  
Vol 59 (6) ◽  
pp. 394
Author(s):  
Teiji Kato ◽  
Takayuki Nakakawaji
Keyword(s):  
Atomic Force Microscopy ◽  
Disk Surface ◽  
Thin Layers ◽  
Solid Surfaces ◽  
Carbon Overcoat ◽  
Magnetic Disk ◽  
Force Microscopy ◽  
Atomic Force ◽  
Pfpe Lubricant ◽  
Atomically Flat

Cryogenic Atomic Force Microscopy (AFM) was used to observe perfluoropolyether (PFPE) lubricant molecules at atomically flat solid surfaces and at a magnetic disk surface to understand the lubricity of ultra-thin (1 nm) lubricant layers at the hard disk surface. Molecular imaging of PFPE lubricant molecules reveals the formation of reversed micelle structures at comparatively non-polar solid surfaces such as gold or the carbon overcoat of magnetic disks.

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