Fatty acid-binding protein3 expression in BeWo cells, a human placental choriocarcinoma cell line

2017 ◽  
Vol 120 ◽  
pp. 1-7 ◽  
Author(s):  
Claire Leroy ◽  
Kari Anne Risan Tobin ◽  
Sanjay Basak ◽  
Anne Cathrine Staff ◽  
Asim K. Duttaroy
Keyword(s):  
Fatty Acid ◽  
Cell Line ◽  
Fatty Acid Binding ◽  
Bewo Cells ◽  
Choriocarcinoma Cell Line

Biological action of keratinocyte growth factor in BeWo cells, a human choriocarcinoma cell line

2000 ◽  
Vol 23 (1) ◽  
pp. 19-22 ◽  
Author(s):  
H. Matsui ◽  
Michiyoshi Taga ◽  
K. Kurogi ◽  
M. Hiraga ◽  
K. Suyama ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Line ◽  
Keratinocyte Growth Factor ◽  
Biological Action ◽  
Bewo Cells ◽  
Choriocarcinoma Cell Line

scholarly journals Regulation of ADRP expression by long-chain polyunsaturated fatty acids in BeWo cells, a human placental choriocarcinoma cell line

2006 ◽  
Vol 47 (4) ◽  
pp. 815-823 ◽  
Author(s):  
Kari Anne Risan Tobin ◽  
Nina Kittelsen Harsem ◽  
Knut Tomas Dalen ◽  
Anne Cathrine Staff ◽  
Hilde Irene Nebb ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Cell Line ◽  
Bewo Cells ◽  
Long Chain ◽  
Choriocarcinoma Cell Line

scholarly journals The choriocarcinoma cell line BeWo: syncytial fusion and expression of syncytium-specific proteins

Reproduction ◽  
2010 ◽  
Vol 140 (5) ◽  
pp. 759-766 ◽  
Author(s):  
Kristina Orendi ◽  
Martin Gauster ◽  
Gerit Moser ◽  
Hamutal Meiri ◽  
Berthold Huppertz
Keyword(s):  
Protein Expression ◽  
Cell Line ◽  
Cell Fusion ◽  
Cell Counting ◽  
Bewo Cell ◽  
Placental Alkaline Phosphatase ◽  
Bewo Cells ◽  
Villous Trophoblast ◽  
Choriocarcinoma Cell Line

Fusion of the trophoblast-derived choriocarcinoma cell line BeWo can be triggered by forskolin. BeWo cells are regularly used as a cell culture model to mimic in vivo syncytialisation of placental villous trophoblast. The β subunit of human chorionic gonadotropin (CGB), placental alkaline phosphatase as well as placental protein 13 (PP13, LGALS13) are exclusively expressed in the syncytiotrophoblast of the human placenta, and CGB is commonly used as a marker of syncytial differentiation. Here we tested the hypothesis that syncytial fusion precedes CGB and LGALS13 expression in trophoblast-derived BeWo cells. BeWo cells were cultured for 48 h in the presence or absence of forskolin and varying concentrations of H-89, a protein kinase A inhibitor that interferes with the forskolin-mediated pathway of syncytial fusion. LGALS13 and CGB expression were quantified by DELFIA and real-time PCR. Cell fusion was determined by morphological analysis and cell counting after immunofluorescence staining. In forskolin-stimulated BeWo cells that were hindered to fuse by treatment with H-89, levels of CGB protein expression were not altered, while LGALS13 protein and mRNA expression decreased significantly to control levels without forskolin. The LGALS13 protein expression data coincided with a significant decrease in syncytial fusion, while CGB protein expression was unaffected by rates of cell fusion and proliferation. We postulate that CGB protein expression is not necessarily linked to syncytial fusion, and thus CGB should be used with great caution as a marker of BeWo cell fusion.

PPARγ2 regulates lipogenesis and lipid accumulation in steatotic hepatocytes

2005 ◽  
Vol 288 (6) ◽  
pp. E1195-E1205 ◽  
Author(s):  
Susan E. Schadinger ◽  
Nancy L. R. Bucher ◽  
Barbara M. Schreiber ◽  
Stephen R. Farmer
Keyword(s):  
Fatty Acid ◽  
Cell Line ◽  
Lipid Accumulation ◽  
Fatty Acid Synthase ◽  
Molecular Mechanisms ◽  
De Novo ◽  
Binding Protein ◽  
Regulatory Element ◽  
Fatty Acid Binding ◽  
Triacylglycerol Synthesis

Peroxisome proliferator-activated receptor-γ (PPARγ) is considered to be one of the master regulators of adipocyte differentiation. PPARγ2 is abundantly expressed in mature adipocytes and is elevated in the livers of animals that develop fatty livers. The aim of this study was to determine the ability of PPARγ2 to induce lipid accumulation in hepatocytes and to delineate molecular mechanisms driving this process. The hepatic cell line AML-12 was used to generate a cell line stably expressing PPARγ2. Oil Red O staining revealed that PPARγ2 induces lipid accumulation in hepatocytes. This phenotype is accompanied by a selective upregulation of several adipogenic and lipogenic genes including adipose differentiation-related protein (ADRP), adipocyte fatty acid-binding protein 4, sterol regulatory element-binding protein-1 (SREBP-1), fatty acid synthase (FAS), and acetyl-CoA carboxylase, genes whose expression levels are known to increase in steatotic livers of ob/ob mice. Furthermore, the PPARγ2-regulated induction of both SREBP-1 and FAS parallels an increase in de novo triacylglycerol synthesis in hepatocytes. Triacylglycerol synthesis and lipid accumulation are further enhanced by culturing hepatocytes with troglitazone in the absence of exogenous lipids. These results correspond with an increase in the lipid droplet protein, ADRP, and the data demonstrate that ADRP functions to coat lipid droplets in hepatocytes as observed by confocal microscopy. Taken together, these observations propose a role for PPARγ2 as an inducer of steatosis in hepatocytes and suggest that this phenomenon occurs through an induction of pathways regulating de novo lipid synthesis.

Ultrastructure of Choriocarcinoma in Vitro

1969 ◽  
Vol 27 ◽  
pp. 362-363
Author(s):  
John C. Garancis ◽  
R. A. Pattillo
Keyword(s):  
Cell Line ◽  
Physiological Function ◽  
Cell System ◽  
Bewo Cell ◽  
Bewo Cells ◽  
Gestational Choriocarcinoma ◽  
Bewo Cell Line

Growth of cell system (BeWo-cell line) derived from human gestational choriocarcinoma has been established and continuously maintained in-vitro. Furthermore, it is evident from the previous studies that this cell line has retained the physiological function of the placental trophoblasts, namely the synthesis of human chorionic gonadotrophil(HCG).The BeWo cells were relatively small and possessed single nuclei, thus indicating that this cell line consists exclusively of cytotrophoblasts. In some instances cells appeared widely separated and their lateral surfaces were provided with numerous microvilli (Fig.1).

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