Biological action of keratinocyte growth factor in BeWo cells, a human choriocarcinoma cell line

2000 ◽  
Vol 23 (1) ◽  
pp. 19-22 ◽  
Author(s):  
H. Matsui ◽  
Michiyoshi Taga ◽  
K. Kurogi ◽  
M. Hiraga ◽  
K. Suyama ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Line ◽  
Keratinocyte Growth Factor ◽  
Biological Action ◽  
Bewo Cells ◽  
Choriocarcinoma Cell Line

Effect of keratinocyte growth factor and activin on cell growth in the human prostatic cancer cell line LNCaP

2004 ◽  
Vol 22 (2) ◽  
Author(s):  
Bernhard Planz ◽  
Horia Oltean ◽  
Thomas Deix ◽  
SandraD. Kirley ◽  
QiFa Wang ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Growth ◽  
Cell Line ◽  
Cancer Cell ◽  
Prostatic Cancer ◽  
Keratinocyte Growth Factor ◽  
Cancer Cell Line

scholarly journals Regulation of ADRP expression by long-chain polyunsaturated fatty acids in BeWo cells, a human placental choriocarcinoma cell line

2006 ◽  
Vol 47 (4) ◽  
pp. 815-823 ◽  
Author(s):  
Kari Anne Risan Tobin ◽  
Nina Kittelsen Harsem ◽  
Knut Tomas Dalen ◽  
Anne Cathrine Staff ◽  
Hilde Irene Nebb ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Cell Line ◽  
Bewo Cells ◽  
Long Chain ◽  
Choriocarcinoma Cell Line

scholarly journals The choriocarcinoma cell line BeWo: syncytial fusion and expression of syncytium-specific proteins

Reproduction ◽  
2010 ◽  
Vol 140 (5) ◽  
pp. 759-766 ◽  
Author(s):  
Kristina Orendi ◽  
Martin Gauster ◽  
Gerit Moser ◽  
Hamutal Meiri ◽  
Berthold Huppertz
Keyword(s):  
Protein Expression ◽  
Cell Line ◽  
Cell Fusion ◽  
Cell Counting ◽  
Bewo Cell ◽  
Placental Alkaline Phosphatase ◽  
Bewo Cells ◽  
Villous Trophoblast ◽  
Choriocarcinoma Cell Line

Fusion of the trophoblast-derived choriocarcinoma cell line BeWo can be triggered by forskolin. BeWo cells are regularly used as a cell culture model to mimic in vivo syncytialisation of placental villous trophoblast. The β subunit of human chorionic gonadotropin (CGB), placental alkaline phosphatase as well as placental protein 13 (PP13, LGALS13) are exclusively expressed in the syncytiotrophoblast of the human placenta, and CGB is commonly used as a marker of syncytial differentiation. Here we tested the hypothesis that syncytial fusion precedes CGB and LGALS13 expression in trophoblast-derived BeWo cells. BeWo cells were cultured for 48 h in the presence or absence of forskolin and varying concentrations of H-89, a protein kinase A inhibitor that interferes with the forskolin-mediated pathway of syncytial fusion. LGALS13 and CGB expression were quantified by DELFIA and real-time PCR. Cell fusion was determined by morphological analysis and cell counting after immunofluorescence staining. In forskolin-stimulated BeWo cells that were hindered to fuse by treatment with H-89, levels of CGB protein expression were not altered, while LGALS13 protein and mRNA expression decreased significantly to control levels without forskolin. The LGALS13 protein expression data coincided with a significant decrease in syncytial fusion, while CGB protein expression was unaffected by rates of cell fusion and proliferation. We postulate that CGB protein expression is not necessarily linked to syncytial fusion, and thus CGB should be used with great caution as a marker of BeWo cell fusion.

Fatty acid-binding protein3 expression in BeWo cells, a human placental choriocarcinoma cell line

2017 ◽  
Vol 120 ◽  
pp. 1-7 ◽  
Author(s):  
Claire Leroy ◽  
Kari Anne Risan Tobin ◽  
Sanjay Basak ◽  
Anne Cathrine Staff ◽  
Asim K. Duttaroy
Keyword(s):  
Fatty Acid ◽  
Cell Line ◽  
Fatty Acid Binding ◽  
Bewo Cells ◽  
Choriocarcinoma Cell Line
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