Transcriptional regulation of pWW0 transfer genes in Pseudomonas putida KT2440

Plasmid ◽  
2004 ◽  
Vol 52 (3) ◽  
pp. 169-181 ◽  
Author(s):  
Lotte M. Lambertsen ◽  
Søren Molin ◽  
Niels Kroer ◽  
Christopher M. Thomas
Keyword(s):  
Transcriptional Regulation ◽  
Pseudomonas Putida ◽  
Pseudomonas Putida Kt2440 ◽  
Transfer Genes

scholarly journals Functional role of lanthanides in enzymatic activity and transcriptional regulation of PQQ-dependent alcohol dehydrogenases in Pseudomonas putida KT2440

2017 ◽  
Author(s):  
Matthias Wehrmann ◽  
Patrick Billard ◽  
Audrey Martin Meriadec ◽  
Asfaw Zegeye ◽  
Janosch Klebensberger
Keyword(s):  
Transcriptional Regulation ◽  
Pseudomonas Putida ◽  
Regulatory Network ◽  
Model Organism ◽  
Adaptive Strategy ◽  
Lanthanide Ions ◽  
Methylotrophic Bacterium ◽  
Methylotrophic Bacteria ◽  
Pseudomonas Putida Kt2440 ◽  
Alcohol Dehydrogenases

ABSTRACTThe oxidation of alcohols and aldehydes is crucial for detoxification and efficient catabolism of various volatile organic compounds (VOCs). Thus, many Gram-negative bacteria have evolved periplasmic oxidation systems, based on pyrroloquinoline quinone-dependent alcohol dehydrogenases (PQQ-ADHs), which are often functionally redundant. Using purified enzymes from the soil-dwelling model organism Pseudomonas putida KT2440, the present study reports the first description and characterization of a lanthanide-dependent PQQ-ADH (PedH) in a non-methylotrophic bacterium. PedH exhibits enzyme activity on a similar substrate range as its Ca2+-dependent counterpart PedE, including linear and aromatic primary and secondary alcohols as well as aldehydes, however, only in the presence of lanthanide ions including La3+, Ce3+, Pr3+, Sm3+ or Nd3+. Reporter assays revealed that PedH not only has a catalytic function, but is also involved in the transcriptional regulation of pedE and pedH, most likely acting as a sensory module. Notably, the underlying regulatory network is responsive to as little as 1 – 10 nM of lanthanum, a concentration assumed to be of ecological relevance. The present study further demonstrates that the PQQ-dependent oxidation system is crucial for efficient growth with a variety of volatile alcohols. From these results we conclude that functional redundancy and inverse regulation of PedE and PedH represents an adaptive strategy of P. putida KT2440 to optimize growth with volatile alcohols in response to different lanthanide availability.IMPORTANCEDue to their low bioavailability, lanthanides have long been considered as biologically inert. In recent years however, the identification of lanthanides as a cofactor in methylotrophic bacteria has attracted tremendous interest among various biological fields. The present study reveals that one of the two PQQ-ADHs produced by the model organism P. putida KT2440 also utilizes lanthanides as a cofactor, thus expanding the scope of lanthanide employing bacteria beyond the methylotrophs. Similar to methyloptrophic bacteria, a complex regulatory network is involved in the lanthanide-responsive switch between the two PQQ-ADHs encoded by P. putida KT2440. We further show that functional production of at least one of the enzymes is crucial for efficient growth with several volatile alcohols. Overall, our study provides a novel understanding for the redundancy of PQQ-ADHs observed in many organisms and further highlights the importance of lanthanides for bacterial metabolism, particularly in soil environments.

scholarly journals The exopolysaccharide gene cluster pea is transcriptionally controlled by RpoS and repressed by AmrZ in Pseudomonas putida KT2440

2019 ◽  
Vol 218 ◽  
pp. 1-11 ◽  
Author(s):  
Huizhong Liu ◽  
Huaduo Yan ◽  
Yujie Xiao ◽  
Hailing Nie ◽  
Qiaoyun Huang ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Gene Cluster ◽  
Pseudomonas Putida Kt2440

scholarly journals Conversion and assimilation of furfural and 5-(hydroxymethyl)furfural by Pseudomonas putida KT2440

2017 ◽  
Vol 4 ◽  
pp. 22-28 ◽  
Author(s):  
Michael T. Guarnieri ◽  
Mary Ann Franden ◽  
Christopher W. Johnson ◽  
Gregg T. Beckham
Keyword(s):  
Pseudomonas Putida ◽  
Pseudomonas Putida Kt2440 ◽  
Hydroxymethyl Furfural

scholarly journals Differential proteomics and physiology of Pseudomonas putida KT2440 under filament-inducing conditions

2012 ◽  
Vol 12 (1) ◽  
pp. 282 ◽  
Author(s):  
Aurélie Crabbé ◽  
Baptiste Leroy ◽  
Ruddy Wattiez ◽  
Abram Aertsen ◽  
Natalie Leys ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Differential Proteomics ◽  
Pseudomonas Putida Kt2440

scholarly journals Genome-wide analysis of the FleQ direct regulon in Pseudomonas fluorescens F113 and Pseudomonas putida KT2440

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Esther Blanco-Romero ◽  
Miguel Redondo-Nieto ◽  
Francisco Martínez-Granero ◽  
Daniel Garrido-Sanz ◽  
Maria Isabel Ramos-González ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Pseudomonas Fluorescens ◽  
Pseudomonas Putida Kt2440 ◽  
Genome Wide Analysis ◽  
Genome Wide
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