Identification and biological evaluation of grapefruit oil components as potential novel efflux pump modulators in methicillin-resistant Staphylococcus aureus bacterial strains

2004 ◽  
Vol 65 (22) ◽  
pp. 3021-3027 ◽  
Author(s):  
Abedel-Nasser Abulrob ◽  
Marc T.E. Suller ◽  
Mark Gumbleton ◽  
Claire Simons ◽  
A. Denver Russell
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Efflux Pump ◽  
Biological Evaluation ◽  
Bacterial Strains ◽  
Methicillin Resistant ◽  
Oil Components

scholarly journals Use of Pulsed-Field Gel Electrophoresis to Determine the Source of Methicillin-Resistant Staphylococcus aureus Bacteremia

2021 ◽  
Vol 13 (3) ◽  
pp. 602-610
Author(s):  
Eugene Y. H. Yeung ◽  
Ivan Gorn
Keyword(s):  
Staphylococcus Aureus ◽  
Gel Electrophoresis ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Epidemiological Studies ◽  
Pelvic Trauma ◽  
Pulsed Field Gel Electrophoresis ◽  
Bacterial Strains ◽  
Methicillin Resistant ◽  
Pulsed Field ◽  
Clinical Cases

Pulsed-field gel electrophoresis (PFGE) has historically been considered the gold standard in fingerprinting bacterial strains in epidemiological studies and outbreak investigations; little is known regarding its use in individual clinical cases. The current study detailed two clinical cases in which PFGE helped to determine the source of their methicillin-resistant Staphylococcus aureus (MRSA) bacteremia. Patient A was found to have MRSA bacteremia after trauma in her pelvic area. MRSA was also found in her groin but not in her nostril and rectum. PFGE was performed that showed variable bands of her MRSA isolates from blood and groin, suggestive of different strains of MRSA. Her MRSA bacteremia was determined to be unrelated to her pelvic trauma. Patient B was found to have MRSA bacteremia after colonoscopy. MRSA was also found in his nostril and rectum. PFGE was performed that showed variable bands of his MRSA isolates from blood and rectum but identical bands of MRSA isolates from his blood and nostril. His MRSA bacteremia was determined to be unrelated to his colonoscopy procedure. The current study demonstrates the use of PFGE to rule out the source of bacteremia in individual clinical cases.

Frequency of efflux pump genes mediating ciprofloxacin and antiseptic resistance in methicillin-resistant Staphylococcus aureus isolates

2017 ◽  
Vol 111 ◽  
pp. 71-74 ◽  
Author(s):  
Sepideh Hassanzadeh ◽  
Rahil Mashhadi ◽  
Masoud Yousefi ◽  
Emran Askari ◽  
Maryam Saniei ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Efflux Pump ◽  
Methicillin Resistant

scholarly journals Auranofin and related heterometallic gold(I)–thiolates as potent inhibitors of methicillin-resistant Staphylococcus aureus bacterial strains

2014 ◽  
Vol 138 ◽  
pp. 81-88 ◽  
Author(s):  
Yozane Hokai ◽  
Boruch Jurkowicz ◽  
Jacob Fernández-Gallardo ◽  
Nuruddinkodja Zakirkhodjaev ◽  
Mercedes Sanaú ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Bacterial Strains ◽  
Methicillin Resistant

Efflux pump inhibitory activity of flavonoids isolated from Alpinia calcarata against methicillin-resistant Staphylococcus aureus

Biologia ◽  
2016 ◽  
Vol 71 (5) ◽  
Author(s):  
Harmandeep K. Randhawa ◽  
Kanwarpreet K. Hundal ◽  
Pallavi N. Ahirrao ◽  
Sanjay M. Jachak ◽  
Hemraj S. Nandanwar
Keyword(s):  
Staphylococcus Aureus ◽  
Inhibitory Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Efflux Pump ◽  
Methicillin Resistant

AbstractMethicillin-resistant

scholarly journals Dihydrostilbenes from Indigofera Pulchra

2008 ◽  
Vol 3 (5) ◽  
pp. 1934578X0800300
Author(s):  
Aliyu Musa ◽  
A. K. Haruna ◽  
M. Ilyas ◽  
Augustine Ahmadu ◽  
Simon Gibbons ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Nmr Spectroscopy ◽  
Structure Elucidation ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Efflux Pump ◽  
2D Nmr ◽  
Multidrug Resistant ◽  
Methicillin Resistant ◽  
Weak Activity

A mixture of two new dihydrostilbenes, 3-methoxy-4,5-methylenedioxy-4′- O-(2ξ,3-dihydroxy-3-methylbutyl)-dihydrostilbene (1) and 3,5-dimethoxy-4′- O-(2ξ,3-dihydroxy-3-methylbutyl)-dihydrostilbene (2), was isolated from Indigofera pulchra as an inseparable mixture. Several isolation strategies, including multiple preparative TLC, SPE and finally HPLC were undertaken in an attempt to separate these metabolites, but to no avail. Structure elucidation on the mixture was conducted and we were able to establish the structures of these compounds unambiguously by MS and a series of 1D and 2D-NMR analyses. The mixture of these dihydrostilbenes was analyzed by NMR spectroscopy and 1 and 2 were in a ratio of 52:48, respectively, and this was evaluated against a panel of methicillin-resistant Staphylococcus aureus (MRSA) and multidrug-resistant variants of this organism, including a strain over-expressing the NorA efflux pump; weak activity was observed.

scholarly journals Recurrent Methicillin-Resistant Staphylococcus aureus Cutaneous Abscesses and Selection of Reduced Chlorhexidine Susceptibility during Chlorhexidine Use

2015 ◽  
Vol 53 (11) ◽  
pp. 3677-3682 ◽  
Author(s):  
Ryan C. Johnson ◽  
Carey D. Schlett ◽  
Katrina Crawford ◽  
Jeffrey B. Lanier ◽  
D. Scott Merrell ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Disease Prevention ◽  
Clinical Isolate ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Efflux Pump ◽  
Methicillin Resistant ◽  
Content Type ◽  
Selection Of

We describe the selection of reduced chlorhexidine susceptibility during chlorhexidine use in a patient with two episodes of cutaneous USA300 methicillin-resistantStaphylococcus aureusabscess. The second clinical isolate harbors a novel plasmid that encodes the QacA efflux pump. Greater use of chlorhexidine for disease prevention warrants surveillance for resistance.

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