scholarly journals Gene expression in the ventral tegmental area of 5 pairs of rat lines selectively bred for high or low ethanol consumption

2012 ◽  
Vol 102 (2) ◽  
pp. 275-285 ◽  
Author(s):  
William J. McBride ◽  
Mark W. Kimpel ◽  
Jeanette N. McClintick ◽  
Zheng-Ming Ding ◽  
Petri Hyytia ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ventral Tegmental Area ◽  
Ethanol Consumption ◽  
Ventral Tegmental

scholarly journals Cocaine induces paradigm-specific changes to the transcriptome within the ventral tegmental area

2021 ◽  
Author(s):  
Rianne R. Campbell ◽  
Siwei Chen ◽  
Joy H. Beardwood ◽  
Alberto J. López ◽  
Lilyana V. Pham ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ventral Tegmental Area ◽  
Home Cage ◽  
Expression Patterns ◽  
Energy Regulation ◽  
Biological Processes ◽  
Cocaine Exposure ◽  
Self Administration ◽  
Drug Reinforcement ◽  
Ventral Tegmental

AbstractDuring the initial stages of drug use, cocaine-induced neuroadaptations within the ventral tegmental area (VTA) are critical for drug-associated cue learning and drug reinforcement processes. These neuroadaptations occur, in part, from alterations to the transcriptome. Although cocaine-induced transcriptional mechanisms within the VTA have been examined, various regimens and paradigms have been employed to examine candidate target genes. In order to identify key genes and biological processes regulating cocaine-induced processes, we employed genome-wide RNA-sequencing to analyze transcriptional profiles within the VTA from male mice that underwent one of four commonly used paradigms: acute home cage injections of cocaine, chronic home cage injections of cocaine, cocaine-conditioning, or intravenous-self administration of cocaine. We found that cocaine alters distinct sets of VTA genes within each exposure paradigm. Using behavioral measures from cocaine self-administering mice, we also found several genes whose expression patterns corelate with cocaine intake. In addition to overall gene expression levels, we identified several predicted upstream regulators of cocaine-induced transcription shared across all paradigms. Although distinct gene sets were altered across cocaine exposure paradigms, we found, from Gene Ontology (GO) term analysis, that biological processes important for energy regulation and synaptic plasticity were affected across all cocaine paradigms. Coexpression analysis also identified gene networks that are altered by cocaine. These data indicate that cocaine alters networks enriched with glial cell markers of the VTA that are involved in gene regulation and synaptic processes. Our analyses demonstrate that transcriptional changes within the VTA depend on the route, dose and context of cocaine exposure, and highlight several biological processes affected by cocaine. Overall, these findings provide a unique resource of gene expression data for future studies examining novel cocaine gene targets that regulate drug-associated behaviors.

scholarly journals Microinjection of Glycine into the Ventral Tegmental Area Selectively Decreases Ethanol Consumption

2012 ◽  
Vol 341 (1) ◽  
pp. 196-204 ◽  
Author(s):  
Jing Li ◽  
Hong Nie ◽  
Weiliang Bian ◽  
Vaidehi Dave ◽  
Patricia H. Janak ◽  
...  
Keyword(s):  
Ventral Tegmental Area ◽  
Ethanol Consumption ◽  
Ventral Tegmental

scholarly journals Changes in gene expression within the ventral tegmental area following repeated excessive binge-like alcohol drinking by alcohol-preferring (P) rats

Alcohol ◽  
2013 ◽  
Vol 47 (5) ◽  
pp. 367-380 ◽  
Author(s):  
William J. McBride ◽  
Mark W. Kimpel ◽  
Jeanette N. McClintick ◽  
Zheng-Ming Ding ◽  
Sheketha R. Hauser ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ventral Tegmental Area ◽  
Alcohol Drinking ◽  
Ventral Tegmental

scholarly journals Epigenetic basis of opiate suppression of Bdnf gene expression in the ventral tegmental area

2015 ◽  
Vol 18 (3) ◽  
pp. 415-422 ◽  
Author(s):  
Ja Wook Koo ◽  
Michelle S Mazei-Robison ◽  
Quincey LaPlant ◽  
Gabor Egervari ◽  
Kevin M Braunscheidel ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ventral Tegmental Area ◽  
Bdnf Gene ◽  
Ventral Tegmental

Cannabidiol prevents priming- and stress-induced reinstatement of the conditioned place preference induced by cocaine in mice

2021 ◽  
pp. 026988112096595
Author(s):  
Claudia Calpe-López ◽  
Ani Gasparyan ◽  
Francisco Navarrete ◽  
Jorge Manzanares ◽  
Jose Miñarro ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ventral Tegmental Area ◽  
Conditioned Place Preference ◽  
Cannabis Sativa ◽  
Social Defeat ◽  
Place Preference ◽  
Cocaine Seeking ◽  
Ventral Tegmental ◽  
Relative Gene ◽  
Reinstatement Test

Background: Cocaine dependence is an important problem without any effective pharmacological treatment. Some preclinical studies have suggested that cannabidiol (CBD), a component of the Cannabis sativa plant, could be useful for the treatment of cocaine use disorders. Aims: This work aims to evaluate the ability of CBD to reduce priming- and stress-induced reinstatement of the conditioned place preference (CPP) induced by cocaine. Methods: Young adult CD-1 male mice were allocated to 10 groups ( n = 12/group), conditioned with cocaine (10 mg/kg) and exposed to extinction of CPP (two sessions per week). When extinction was achieved, each group received the corresponding treatment before the reinstatement test. In experiment 1, six groups were used: vehicle+saline (Veh+Sal), 5 mg/kg cocaine alone (Veh+Coc) or with CBD 30 or 60 mg/kg (CBD30+Coc, CBD60+Coc) and CBD alone (CBD30+Sal, CBD60+Sal). In experiment 2, four groups were used: exploration (Veh+Expl), social defeat (Veh+SD) and social defeat with CBD (CBD30+SD and CBD60+SD). Furthermore, the relative gene expression of the dopamine transporter (DAT) in the ventral tegmental area was measured. Results: All mice acquired cocaine CPP and extinguished it after three or four weeks. Only the groups treated with cocaine priming (Veh+Coc) or exposed to social defeat (Veh+SD) showed reinstatement of CPP. Interestingly, CBD itself did not induce reinstatement and blocked the reinstating effects of cocaine priming and social defeat. Furthermore, cocaine priming increased DAT gene expression in the ventral tegmental area and CBD completely reversed this effect. Conclusion: These results suggest that CBD could reduce reinstatement to cocaine seeking after a period of abstinence.

scholarly journals Molecular implications of prolonged aggression experience: Th, Dat1, Snca and Bdnf gene expression in the ventral tegmental area of the victorious male mice

2008 ◽  
Author(s):  
Natalia P. Bondar ◽  
Ul’yana A. Boyarskikh ◽  
Irina L. Kovalenko ◽  
Maxim L. Filipenko ◽  
Natalia Kudryavtseva
Keyword(s):  
Gene Expression ◽  
Ventral Tegmental Area ◽  
Male Mice ◽  
Bdnf Gene ◽  
Ventral Tegmental
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