d-Amphetamine-induced cytotoxicity and oxidative stress in isolated rat hepatocytes

2011 ◽  
Vol 18 (4) ◽  
pp. 279-285 ◽  
Author(s):  
Osama S. El-Tawil ◽  
Ali H. Abou-Hadeed ◽  
Mohamed F. EL-Bab ◽  
Abeir A. Shalaby
Keyword(s):  
Oxidative Stress ◽  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
And Oxidative Stress ◽  
Isolated Rat

scholarly journals Protective Effects of Saponin Mixture, Isolated from <i>Astragalus monspessulanus</i> subsp. <i>monspessulanus</i> on Tert-Butyl Hydroperoxide—Induced Oxidative Stress in Isolated Rat Hepatocytes

2015 ◽  
Vol 06 (06) ◽  
pp. 799-803 ◽  
Author(s):  
Magdalena Spasova Kondeva-Burdina ◽  
Viktor Bratkov ◽  
Rumyana Lubomirova Simeonova ◽  
Vessela Bisserova Vitcheva ◽  
Ilina Nikolaeva Krasteva ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rat Hepatocytes ◽  
Protective Effects ◽  
Isolated Rat Hepatocytes ◽  
Butyl Hydroperoxide ◽  
Tert Butyl ◽  
Tert Butyl Hydroperoxide ◽  
Isolated Rat

The protective effects of melanoidins in adriamycin-induced oxidative stress in isolated rat hepatocytes

2004 ◽  
Vol 84 (13) ◽  
pp. 1701-1707 ◽  
Author(s):  
V Valls-Bellés ◽  
MC Torres ◽  
P Muñiz ◽  
L Boix ◽  
ML González-Sanjose ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rat Hepatocytes ◽  
Protective Effects ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat

Protective effects of fungal β-(1→3)-D-glucan against oxidative stress cytotoxicity induced by depleted uranium in isolated rat hepatocytes

2010 ◽  
Vol 30 (3) ◽  
pp. 173-181 ◽  
Author(s):  
Jalal Pourahmad ◽  
Fatemeh Shaki ◽  
Farahnaz Tanbakosazan ◽  
Ruhollah Ghalandari ◽  
Hossein Ali Ettehadi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Rat Hepatocytes ◽  
Depleted Uranium ◽  
Glutathione Depletion ◽  
Scavenging Activity ◽  
Isolated Rat Hepatocytes ◽  
Membrane Rupture ◽  
Isolated Rat

Previous reports suggested that certain carbohydrate polymers, such as β-(1→3)-D-glucan, may possess free radical scavenging activity. The present study examined the free radical scavenging activity of a carbohydrate polymer, β-(1→3)-D-glucan against oxidative stress induced by depleted uranium in isolated rat hepatocytes. Addition of U (VI) (uranyl acetate) to isolated rat hepatocytes results in reactive oxygen species (ROS) formation, rapid glutathione depletion, mitochondrial membrane potential collapse and lysosomal membrane rupture before hepatocyte lysis occurred. Our results showed that quite similar to silymarin, which is a known antioxidant and radical scavenger, tiny concentration of β-glucan (138 nM) very successfully protected the hepatocytes against cell lysis and all oxidative stress cytotoxicity endpoints caused by depleted uranium including ROS formation, glutathione depletion, decreased mitochondrial membrane potential, lysosomal membrane rupture and caspase 3 activity increase. In conclusion, our results confirmed the antioxidant and radical scavenging activity of β-(1→3)-D-glucan and suggested this compound and silymarin as possible drug candidates for prophylaxis and treatment against depleted uranium toxic effects.

The biosynthesis of ascorbate protects isolated rat hepatocytes from cumene hydroperoxide-mediated oxidative stress

2005 ◽  
Vol 38 (7) ◽  
pp. 867-873 ◽  
Author(s):  
Tom S. Chan ◽  
Nandita Shangari ◽  
John X. Wilson ◽  
Helen Chan ◽  
Roger F. Butterworth ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cumene Hydroperoxide ◽  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat

Nitrofurantoin-mediated oxidative stress cytotoxicity in isolated rat hepatocytes

1988 ◽  
Vol 37 (16) ◽  
pp. 3109-3117 ◽  
Author(s):  
Luisa Rossi ◽  
Jose M. Silva ◽  
Larry G. McGirr ◽  
Peter J. O'Brien
Keyword(s):  
Oxidative Stress ◽  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat

scholarly journals Effects of aniso-osmolarity and hydroperoxides on intracellular pH in isolated rat hepatocytes as assessed by (2′,7′)-bis(carboxyethyl)-5(6)-carboxyfluorescein and fluorescein isothiocyanate-dextran fluorescence

1994 ◽  
Vol 303 (1) ◽  
pp. 113-120 ◽  
Author(s):  
R Schreiber ◽  
B Stoll ◽  
F Lang ◽  
D Häussinger
Keyword(s):  
Fluorescein Isothiocyanate ◽  
Rat Hepatocytes ◽  
Cell Swelling ◽  
Cell Shrinkage ◽  
Isolated Rat Hepatocytes ◽  
Proton Gradients ◽  
Fluorescein Isothiocyanate Dextran ◽  
And Oxidative Stress ◽  
Vesicular Compartment ◽  
Isolated Rat

Freshly isolated rat hepatocytes were plated for 4-6 h and either loaded with (2′,7)-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) or allowed to endocytose fluorescein isothiocyanate (FITC)-coupled dextran in order to study the effects of aniso-osmotic exposure and oxidative stress on cytosolic (pHcyt) and apparent vesicular pH (pHves) by single-cell fluorescence recordings. In the presence of normo-osmotic (305 mosmol/l) medium pHcyt was 7.23 +/- 0.03 (n = 108), whereas an apparent pH of 6.07 +/- 0.02 (n = 156) was found in the vesicular compartment accessible to endocytosed FITC-dextran. Substitution of 60 mM NaCl against 120 mM raffinose had no effect on pHcyt or apparent pHves, whereas addition of NH4Cl increased both pHcyt and apparent pHves. Hypo-osmotic cell swelling lowered pHcyt, whereas simultaneously apparent pHves increased. These effects were rapidly reversible upon re-institution of normo-osmotic media. Similarly, an increase of apparent pHves was observed when cell swelling was induced by Ba2+, glutamine or histidine. Conversely, hyperosmotic cell shrinkage due to addition of NaCl or raffinose led to a cytosolic alkalinization and a vesicular acidification. Both, H2O2 (0.2 mmol/l) and t-butyl-hydroperoxide (0.2 mmol/l) were without effect on pHcyt, but lowered apparent pHves by about 0.2 pH units. Ba2+ (1 mmol/l) diminished the acidifying effect of the hydroperoxides by about 50%. Pretreatment of the cells with colchicine, but not with lumicolchicine, largely abolished the effects of aniso-osmolarity and hydroperoxides on pHves. The data suggest that hepatocellular hydration affects the proton gradients built up across the membranes of endocytotic FITC-dextran-accessible compartments in a microtubule-dependent way. They further suggest that hydroperoxides induce vesicular acidification in a colchicine- and Ba(2+)-sensitive way. Because hydroperoxides induce Ba(2+)-sensitive cell shrinkage [Hallbrucker, Ritter, Lang, Gerok and Häussinger (1992) Eur. J. Biochem. 211, 449-458], the results are compatible with the view that hydroperoxide-induced cell shrinkage mediates vesicular acidification. It is concluded that modulation of vesicular pH by the hepatocellular hydration state may play a role in triggering some metabolic changes in response to cell swelling/shrinkage.

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