scholarly journals Protective Effects of Saponin Mixture, Isolated from <i>Astragalus monspessulanus</i> subsp. <i>monspessulanus</i> on Tert-Butyl Hydroperoxide—Induced Oxidative Stress in Isolated Rat Hepatocytes

2015 ◽  
Vol 06 (06) ◽  
pp. 799-803 ◽  
Author(s):  
Magdalena Spasova Kondeva-Burdina ◽  
Viktor Bratkov ◽  
Rumyana Lubomirova Simeonova ◽  
Vessela Bisserova Vitcheva ◽  
Ilina Nikolaeva Krasteva ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rat Hepatocytes ◽  
Protective Effects ◽  
Isolated Rat Hepatocytes ◽  
Butyl Hydroperoxide ◽  
Tert Butyl ◽  
Tert Butyl Hydroperoxide ◽  
Isolated Rat

Tert-Butyl Hydroperoxide-Induced Injury In Isolated Rat Hepatocytes: A Model for Studying Anti-Hepatotoxic Crude Drugs

Planta Medica ◽  
1990 ◽  
Vol 56 (02) ◽  
pp. 171-174 ◽  
Author(s):  
Michel Joyeux ◽  
Alain Rolland ◽  
Jacques Fleurentin ◽  
François Mortier ◽  
Pierre Dorfman
Keyword(s):  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Butyl Hydroperoxide ◽  
Tert Butyl ◽  
Tert Butyl Hydroperoxide ◽  
Crude Drugs ◽  
Isolated Rat

Protective activity of Hypericum androsaemum infusion against tert-butyl hydroperoxide-induced oxidative damage in isolated rat hepatocytes

2004 ◽  
Vol 92 (1) ◽  
pp. 79-84 ◽  
Author(s):  
Patrı́cia Valentão ◽  
Márcia Carvalho ◽  
Eduarda Fernandes ◽  
Félix Carvalho ◽  
Paula Branquinho Andrade ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Rat Hepatocytes ◽  
Protective Activity ◽  
Isolated Rat Hepatocytes ◽  
Butyl Hydroperoxide ◽  
Tert Butyl ◽  
Tert Butyl Hydroperoxide ◽  
Isolated Rat

The protective effects of melanoidins in adriamycin-induced oxidative stress in isolated rat hepatocytes

2004 ◽  
Vol 84 (13) ◽  
pp. 1701-1707 ◽  
Author(s):  
V Valls-Bellés ◽  
MC Torres ◽  
P Muñiz ◽  
L Boix ◽  
ML González-Sanjose ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rat Hepatocytes ◽  
Protective Effects ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat

Protective effects of hesperidin against oxidative stress of tert-butyl hydroperoxide in human hepatocytes

2010 ◽  
Vol 48 (10) ◽  
pp. 2980-2987 ◽  
Author(s):  
Mingcang Chen ◽  
Honggang Gu ◽  
Yiyi Ye ◽  
Bing Lin ◽  
Lijuan Sun ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Hepatocytes ◽  
Protective Effects ◽  
Butyl Hydroperoxide ◽  
Tert Butyl ◽  
Tert Butyl Hydroperoxide

Survival and Metabolic Function of Freshly Isolated Rat Hepatocytes Exposed First to a Heat Shock and Then to an Oxidative Stress

1999 ◽  
Vol 18 (4) ◽  
pp. 239-244
Author(s):  
Isabelle Latour ◽  
Pedro Buc-Calderon
Keyword(s):  
Oxidative Stress ◽  
Heat Shock ◽  
Reduced Glutathione ◽  
Rat Hepatocytes ◽  
Cell Types ◽  
Isolated Rat Hepatocytes ◽  
Tert Butyl Hydroperoxide ◽  
Shock Proteins ◽  
Freshly Isolated Rat Hepatocytes ◽  
Isolated Rat

The formation of heat shock proteins (hsp) leading to thermotolerance has been extensively reported in many cell types. In freshly isolated rat hepatocytes, hsp were synthesized after 60 minutes of incubation at 42°C. Cell survival was not modified by such a treatment, but protein synthesis, secretion of triglycerides as lipoproteins, and the maintenance of both ATP and glycogen levels were significantly impaired. When exposed to an oxidative stress, heat-shocked hepatocytes were not more resistant than cells always kept at 37°C. Conversely, the addition of tert-butyl hydroperoxide (tBOOH) resulted, in general, in an increased lactate dehydrogenase leakage. The metabolism of tBOOH, as estimated by the reduced glutathione (GSH) content and GSH peroxidase activity, was similar in both control and heat-shocked hepatocytes. Despite the synthesis of hsp in rat hepatocytes, the lack of resistance to a subsequent oxidant injury may be due to the metabolic impairment caused by the heat shock.

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