Post mortem biochemistry differences between vitreous humor and cerebrospinal fluid

Pathology ◽  
2019 ◽  
Vol 51 ◽  
pp. S115
Author(s):  
Jack Garland ◽  
Winston Philcox ◽  
Sinead McCarthy ◽  
Kilak Kesha ◽  
Leo Lam ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Vitreous Humor ◽  
Post Mortem

1,5-Anhydro-d-glucitol in vitreous humor and cerebrospinal fluid — A helpful tool for identification of diabetes and diabetic coma post mortem

2018 ◽  
Vol 289 ◽  
pp. 397-407 ◽  
Author(s):  
Konrad Sydow ◽  
Theresa Kueting ◽  
Frank Musshoff ◽  
Burkhard Madea ◽  
Cornelius Hess
Keyword(s):  
Cerebrospinal Fluid ◽  
Vitreous Humor ◽  
Diabetic Coma ◽  
Post Mortem ◽  
Helpful Tool

scholarly journals Alteration in the Cerebrospinal Fluid Lipidome in Parkinson’s Disease: A Post-Mortem Pilot Study

Biomedicines ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 491
Author(s):  
Joaquín Fernández-Irigoyen ◽  
Paz Cartas-Cejudo ◽  
Marta Iruarrizaga-Lejarreta ◽  
Enrique Santamaría
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Cerebrospinal Fluid ◽  
Ultra Performance Liquid Chromatography ◽  
Small Population ◽  
Control Group ◽  
Post Mortem ◽  
Cellular Models ◽  
Flight Mass Spectrometry ◽  
Lipid Species

Lipid metabolism is clearly associated to Parkinson’s disease (PD). Although lipid homeostasis has been widely studied in multiple animal and cellular models, as well as in blood derived from PD individuals, the cerebrospinal fluid (CSF) lipidomic profile in PD remains largely unexplored. In this study, we characterized the post-mortem CSF lipidomic imbalance between neurologically intact controls (n = 10) and PD subjects (n = 20). The combination of dual extraction with ultra-performance liquid chromatography-electrospray ionization quadrupole-time-of-flight mass spectrometry (UPLC-ESI-qToF-MS/MS) allowed for the monitoring of 257 lipid species across all samples. Complementary multivariate and univariate data analysis identified that glycerolipids (mono-, di-, and triacylglycerides), saturated and mono/polyunsaturated fatty acids, primary fatty amides, glycerophospholipids (phosphatidylcholines, phosphatidylethanolamines), sphingolipids (ceramides, sphingomyelins), N-acylethanolamines and sterol lipids (cholesteryl esters, steroids) were significantly increased in the CSF of PD compared to the control group. Interestingly, CSF lipid dyshomeostasis differed depending on neuropathological staging and disease duration. These results, despite the limitation of being obtained in a small population, suggest extensive CSF lipid remodeling in PD, shedding new light on the deployment of CSF lipidomics as a promising tool to identify potential lipid markers as well as discriminatory lipid species between PD and other atypical parkinsonisms.

Ante Mortem Cerebrospinal Fluid Levels of Calsyntenin-1 and Neurexin-2a Reflect Post-Mortem TDP-43 Pathology in a Pathological Cohort of Frontotemporal Lobar Degeneration

2021 ◽  
Author(s):  
Alba Cervantes-González ◽  
David J Irwin ◽  
Daniel Alcolea ◽  
Corey T McMillan ◽  
Alice Chen-Plotkin ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Frontotemporal Lobar Degeneration ◽  
Neurofibrillary Tangle ◽  
Surrogate Marker ◽  
Quantitative Measure ◽  
Synaptic Proteins ◽  
Analysis Of Covariance ◽  
Early Event ◽  
Post Mortem ◽  
Csf Levels

Abstract Background. Frontotemporal dementia (FTD) clinical diagnosis is challenged by the variable correspondence between the clinical syndrome and underlying neuropathological changes, the phenotypic overlap with Alzheimer's disease (AD) and the lack of pathophysiologic diagnostic biomarkers. As synapse degeneration is an early event in pathological frontotemporal lobar degeneration (FTLD), a surrogate marker of synapse loss could be used to monitor early pathologic changes. The aim of this study was to evaluate the relationship of antemortem cerebrospinal fluid (CSF) levels of 9 synaptic proteins with postmortem global tau and TDP-43 burden in a neuropathological FTLD cohort. Methods. We included patients with a neuropathological confirmation of FTLD-Tau (n=24, mean age-at-CSF 67 years+/-11), FTLD-TDP (n=25, 66 years+/-9) or AD (n=25, 73 years+/-6) as well as cognitively normal controls (n=35, 69 years+/-7) from the Penn FTD Center and ADRC. A subset of 39 FTLD patients presented without AD comorbidity (neurofibrillary tangle score of B0/B1 according to the NIA-AA classification) and 18 AD patients presented without TDP-43 comorbidity. We quantified the synaptic panel in antemortem CSF by targeted mass spectrometry using isotopically-labeled peptides as internal standards. We used a semi-quantitative measure of tau and TDP-43 inclusions to quantify pathological burden across 16 brain regions. Statistical methods included Spearman rank correlations, one-way analysis of covariance (controlling for sex and age-at-CSF), linear regression (controlling for age-at-death) and receiver-operating characteristic curves. Result. CSF calsyntenin-1 and neurexin-2a were correlated in all patient groups (rs=.55 to .88). In FTLD-TDP, we observed low antemortem CSF levels of calsyntenin-1 and neurexin-2a compared to AD (.72-fold, p=.001, .77-fold, p=.04, respectively) and controls (.80-fold, p=.02, .78-fold, p=.02, respectively), which were inversely associated with post-mortem global TDP-43 burden (regression r2=.36, p=.04 and r2=.35, p=.04, respectively). Neurexin-2a showed 75.0% (95% CI 60-88) accuracy to discriminate FTLD-TDP from controls. Calsyntenin-1 showed 75.1% (95% CI 62-87) accuracy to discriminate FTLD-TDP from AD. None of the synaptic proteins were altered in FTLD-Tau compared to controls (0.79 to 1.12-fold, p>.12) or associated with post-mortem global tau burden (r2=.12, p=.36). Comorbidity had a limited effect on these findings. Conclusion. CSF calsyntenin-1 and neurexin-2a have potential as objective measures of TDP-43-mediated degeneration in FTLD.

Thanatobiochemistry: post mortem study of the vitreous humor for the diagnosis of diabetic ketoacidosis death

2018 ◽  
Vol 76 (3) ◽  
pp. 245-250
Author(s):  
Guillaume Rousseau ◽  
Nicolas Bergerat ◽  
Guillaume Drevin ◽  
Pascal Reynier ◽  
Nathalie Jousset
Keyword(s):  
Diabetic Ketoacidosis ◽  
Vitreous Humor ◽  
Post Mortem

scholarly journals Natriuretic Peptides in Post-mortem Brain Tissue and Cerebrospinal Fluid of Non-demented Humans and Alzheimer’s Disease Patients

2018 ◽  
Vol 12 ◽  
Author(s):  
Simin Mahinrad ◽  
Marjolein Bulk ◽  
Isabelle van der Velpen ◽  
Ahmed Mahfouz ◽  
Willeke van Roon-Mom ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Cerebrospinal Fluid ◽  
Brain Tissue ◽  
Natriuretic Peptides ◽  
Post Mortem ◽  
Post Mortem Brain

Comparison of Fluconazole Pharmacokinetics in Serum, Aqueous Humor, Vitreous Humor, and Cerebrospinal Fluid Following a Single Dose and at Steady State

1998 ◽  
Vol 14 (5) ◽  
pp. 459-471 ◽  
Author(s):  
UMAR K. MIAN ◽  
MARTIN MAYERS ◽  
YOGENDER GARG ◽  
QING-FENG LIU ◽  
GIRARD NEWCOMER ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Single Dose ◽  
Steady State ◽  
Aqueous Humor ◽  
Vitreous Humor

scholarly journals The radiodensity of cerebrospinal fluid and vitreous humor as indicator of the time since death

2016 ◽  
Vol 12 (3) ◽  
pp. 248-256 ◽  
Author(s):  
Desirée H. J. L. M. Koopmanschap ◽  
Alireza R. Bayat ◽  
Bela Kubat ◽  
Henri M. de Bakker ◽  
Mathias W. M. Prokop ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Vitreous Humor ◽  
Time Since Death
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