Dynamic proteomic analysis of protein expression profiles in whole brain of Balb/c mice subjected to unpredictable chronic mild stress: Implications for depressive disorders and future therapies

2011 ◽  
Vol 58 (8) ◽  
pp. 904-913 ◽  
Author(s):  
Yanyong Liu ◽  
Nan Yang ◽  
Wenyu Hao ◽  
Qing Zhao ◽  
Tianyi Ying ◽  
...  
Keyword(s):  
Protein Expression ◽  
Proteomic Analysis ◽  
Depressive Disorders ◽  
Expression Profiles ◽  
Chronic Mild Stress ◽  
Mild Stress ◽  
Whole Brain ◽  
Unpredictable Chronic Mild Stress ◽  
Protein Expression Profiles

scholarly journals Utility of the chronic unpredictable mild stress model in research on new antidepressants

2014 ◽  
Vol 27 (2) ◽  
pp. 97-101 ◽  
Author(s):  
Karolina Pekala ◽  
Barbara Budzynska ◽  
Grazyna Biala
Keyword(s):  
Depressive Disorders ◽  
Prolonged Exposure ◽  
Chronic Mild Stress ◽  
Depressive Illness ◽  
Accurate Diagnosis ◽  
Mild Stress ◽  
Stress Model ◽  
Chronic Unpredictable Mild Stress ◽  
Unpredictable Chronic Mild Stress ◽  
Animal Model Of Depression

Abstract Unpredictable chronic mild stress model was developed as an animal model of depression more than 20 years ago. Essential for this model is that after prolonged exposure of tested animals to a series of unpredictable mild stressors, a condition similar to anhedonia develops, which is observed in the majority of depressive disorders. Unpredictable chronic mild stress model is used nowadays in numerous studies related to the neurobiological and biochemical changes associated with depressive illness. Their results confirm that chronic unpredictable mild stress induces in tested animals a number of changes, which reflect those seen in depressive disorders. Because the effects of unpredictable chronic mild stress can be used in a more accurate diagnosis of the pathophysiology of depressive illness and expand knowledge of its pharmacotherapy, therefore research in this area has been continued all the time.

Proteomic Analysis Identifies Outcome-Predictive Clusters in Patients with Peripheral T-Cell Lymphoma, Not Otherwise Specified

Blood ◽  
2014 ◽  
Vol 124 (21) ◽  
pp. 1623-1623
Author(s):  
Maja Ludvigsen ◽  
Martin Bjerregaard Pedersen ◽  
Stephen Jacques Hamilton-Dutoit ◽  
Knud Bendix ◽  
Michael Boe Møller ◽  
...  
Keyword(s):  
Cluster Analysis ◽  
T Cell ◽  
Protein Expression ◽  
Proteomic Analysis ◽  
Cell Lymphoma ◽  
Expression Profiles ◽  
Peripheral T Cell Lymphoma ◽  
Not Otherwise Specified ◽  
Protein Expression Profiles ◽  
Unsupervised Cluster Analysis

Abstract Introduction: Peripheral T-cell lymphoma, not otherwise specified (PTCL-NOS) is a heterogeneous group of mature T-cell lymphomas, probably composed by different biologically related subsets that have not yet been conclusively identified. In the WHO classification, PTCL-NOS accounts for 25-30% of all mature T-/NK-cell malignancies. The clinical outcome is generally poor with a 5-yr overall survival of 30-35% after conventional treatment strategies. The aim of the study was to apply proteomic analysis in PTCL-NOS and to use the protein expression profiles to characterize clinically relevant subsets within this heterogeneous entity by means of unsupervised cluster analysis. Methods: Archival frozen tumor tissue samples from 20 patients diagnosed with PTCL-NOS from 1991 to 2010 were analyzed for protein expression by high-resolution two-dimensional gel electrophoresis. Individual protein spots were visualized with fluorescence staining and the expression profiles were identified. All patients were homogeneously treated with curatively intended anthracycline-containing combination regimens. Clinico-pathological features were obtained from the Danish Lymphoma Registry (LYFO) and from patient records. Hyperplastic tonsils from healthy adults were included as reference tissue (n=8). Principal component analysis and unsupervised hierarchical cluster analysis were performed on the basis of the protein expression profiles. Differentially expressed (two-fold or higher, Mann-Whitney U-test) proteins between the detected clusters were identified by liquid chromatography - tandem mass spectrometry. Results: Unsupervised cluster analysis defined three distinct clusters: one containing all reference samples and two additional ones further subdividing the PTCL-NOS cases in two separate subsets. Patients from these two PTCL-NOS subsets had significantly different responses to treatment and survival (p = 0.001). The differentially expressed proteins were primarily involved in (i) promotion of tumor growth, (ii) regulation of cellular metabolism, and (iii) immune responses. Conclusion : Proteomic analysis identified shared protein expression patterns and potential prognostic markers in subsets of PTCL-NOS. Disclosures No relevant conflicts of interest to declare.

scholarly journals A Comparison of Isolation Stress and Unpredictable Chronic Mild Stress for the Establishment of Mouse Models of Depressive Disorder

2021 ◽  
Vol 14 ◽  
Author(s):  
Jin-Seok Lee ◽  
Ji-Yun Kang ◽  
Chang-Gue Son
Keyword(s):  
Animal Models ◽  
Depressive Disorder ◽  
Depressive Disorders ◽  
Chronic Mild Stress ◽  
Tail Suspension Test ◽  
Modern Society ◽  
Mild Stress ◽  
Isolation Stress ◽  
Unpredictable Chronic Mild Stress ◽  
Serum Corticosterone

This study aimed to help to understand the influence of stress on depression, which reflects the social environments of especially solitary life and the increasing prevalence of depressive disorders. To determine the distinguishable features of two-representative animal models of stress-induced depressive disorder, we compared isolation stress (IS) and unpredictable chronic mild stress (UCMS). After 4-week of stress, both models showed significant depressive- and anxiety-like behaviors in an open field test (OFT; p < 0.01 for IS, p < 0.01 for UCMS), forced swimming test (FST; p < 0.01 for IS, p < 0.01 for UCMS), and tail suspension test (TST; p < 0.01 for IS, p < 0.05 for UCMS) along with alterations in serum corticosterone levels, serotonin activity in the dorsal raphe nuclei (DRN) and microglial activity in the dentate gyrus of the hippocampus (p < 0.05 for both parameters). In a comparison of the two stress models, IS strongly induced depressive and anxiety features, as indicated by all parameters: behavior test scores (p < 0.05 for OFT, FST, and TST), serum corticosterone levels (p < 0.05), immunohistological alterations for serotonin activity (p < 0.05) and microglial activity (p = 0.072). Our results indicate the suitability of IS for the development of animal models of depressive disorders and may reveal the medical impact of social isolation environment in modern society.

Different protein expression profiles in cheese and clinical isolates of Enterococcus faecalis revealed by proteomic analysis

PROTEOMICS ◽  
2012 ◽  
Vol 12 (3) ◽  
pp. 431-447 ◽  
Author(s):  
Alessandro Pessione ◽  
Cristina Lamberti ◽  
Luca Cocolin ◽  
Simona Campolongo ◽  
Alexander Grunau ◽  
...  
Keyword(s):  
Protein Expression ◽  
Enterococcus Faecalis ◽  
Proteomic Analysis ◽  
Expression Profiles ◽  
Clinical Isolates ◽  
Protein Expression Profiles

Proteins differentially expressed in conidia and mycelia of the entomopathogenic fungusMetarhizium anisopliaesensu stricto

2013 ◽  
Vol 59 (7) ◽  
pp. 443-448 ◽  
Author(s):  
Yubin Su ◽  
Qingfeng Guo ◽  
Jie Tu ◽  
Xiaoxia Li ◽  
Lixue Meng ◽  
...  
Keyword(s):  
Protein Expression ◽  
Proteomic Analysis ◽  
Developmental Stages ◽  
Expression Profiles ◽  
Laser Desorption Ionization ◽  
Appressorium Formation ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Protective Processes ◽  
Protein Expression Profiles

Metarhizium anisopliae is a well-characterized entomopathogenic fungus that attacks a variety of insects. Its conidia are involved in its propagation and also in its infection of host insects. To investigate the protein expression profiles and to identify the proteins related to development and pathogenesis, we performed a comparative proteomic analysis of the conidia and mycelia of an M. anisopliae strain (Ma1291). The analysis used 2-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. We detected 898 ± 37 protein spots in conidia and 1072 ± 24 in mycelia of strain Ma1291. A comparison of the 2 protein-expression profiles indicated that only 28% of protein spots were common to both developmental stages. Finally, we identified 30 proteins (19 from conidia and 11 from mycelia). The identified proteins exclusive to conidia were those involved in protective processes, appressorium formation, and degradation of the host cuticle (protease PR1H). The identified proteins exclusive to mycelia included major proteins participating in biosynthetic and energy metabolism, such as UTP-glucose-1-phosphate uridylyltransferase and heat shock protein 70. This research provides the first proteomic analysis of different developmental stages of M. anisopliae, and the results should facilitate clarification of the molecular basis of these epigenetic variations.

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