Different protein expression profiles in cheese and clinical isolates of Enterococcus faecalis revealed by proteomic analysis

PROTEOMICS ◽  
2012 ◽  
Vol 12 (3) ◽  
pp. 431-447 ◽  
Author(s):  
Alessandro Pessione ◽  
Cristina Lamberti ◽  
Luca Cocolin ◽  
Simona Campolongo ◽  
Alexander Grunau ◽  
...  
Keyword(s):  
Protein Expression ◽  
Enterococcus Faecalis ◽  
Proteomic Analysis ◽  
Expression Profiles ◽  
Clinical Isolates ◽  
Protein Expression Profiles

Proteomic Analysis Identifies Outcome-Predictive Clusters in Patients with Peripheral T-Cell Lymphoma, Not Otherwise Specified

Blood ◽  
2014 ◽  
Vol 124 (21) ◽  
pp. 1623-1623
Author(s):  
Maja Ludvigsen ◽  
Martin Bjerregaard Pedersen ◽  
Stephen Jacques Hamilton-Dutoit ◽  
Knud Bendix ◽  
Michael Boe Møller ◽  
...  
Keyword(s):  
Cluster Analysis ◽  
T Cell ◽  
Protein Expression ◽  
Proteomic Analysis ◽  
Cell Lymphoma ◽  
Expression Profiles ◽  
Peripheral T Cell Lymphoma ◽  
Not Otherwise Specified ◽  
Protein Expression Profiles ◽  
Unsupervised Cluster Analysis

Abstract Introduction: Peripheral T-cell lymphoma, not otherwise specified (PTCL-NOS) is a heterogeneous group of mature T-cell lymphomas, probably composed by different biologically related subsets that have not yet been conclusively identified. In the WHO classification, PTCL-NOS accounts for 25-30% of all mature T-/NK-cell malignancies. The clinical outcome is generally poor with a 5-yr overall survival of 30-35% after conventional treatment strategies. The aim of the study was to apply proteomic analysis in PTCL-NOS and to use the protein expression profiles to characterize clinically relevant subsets within this heterogeneous entity by means of unsupervised cluster analysis. Methods: Archival frozen tumor tissue samples from 20 patients diagnosed with PTCL-NOS from 1991 to 2010 were analyzed for protein expression by high-resolution two-dimensional gel electrophoresis. Individual protein spots were visualized with fluorescence staining and the expression profiles were identified. All patients were homogeneously treated with curatively intended anthracycline-containing combination regimens. Clinico-pathological features were obtained from the Danish Lymphoma Registry (LYFO) and from patient records. Hyperplastic tonsils from healthy adults were included as reference tissue (n=8). Principal component analysis and unsupervised hierarchical cluster analysis were performed on the basis of the protein expression profiles. Differentially expressed (two-fold or higher, Mann-Whitney U-test) proteins between the detected clusters were identified by liquid chromatography - tandem mass spectrometry. Results: Unsupervised cluster analysis defined three distinct clusters: one containing all reference samples and two additional ones further subdividing the PTCL-NOS cases in two separate subsets. Patients from these two PTCL-NOS subsets had significantly different responses to treatment and survival (p = 0.001). The differentially expressed proteins were primarily involved in (i) promotion of tumor growth, (ii) regulation of cellular metabolism, and (iii) immune responses. Conclusion : Proteomic analysis identified shared protein expression patterns and potential prognostic markers in subsets of PTCL-NOS. Disclosures No relevant conflicts of interest to declare.

Proteins differentially expressed in conidia and mycelia of the entomopathogenic fungusMetarhizium anisopliaesensu stricto

2013 ◽  
Vol 59 (7) ◽  
pp. 443-448 ◽  
Author(s):  
Yubin Su ◽  
Qingfeng Guo ◽  
Jie Tu ◽  
Xiaoxia Li ◽  
Lixue Meng ◽  
...  
Keyword(s):  
Protein Expression ◽  
Proteomic Analysis ◽  
Developmental Stages ◽  
Expression Profiles ◽  
Laser Desorption Ionization ◽  
Appressorium Formation ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Protective Processes ◽  
Protein Expression Profiles

Metarhizium anisopliae is a well-characterized entomopathogenic fungus that attacks a variety of insects. Its conidia are involved in its propagation and also in its infection of host insects. To investigate the protein expression profiles and to identify the proteins related to development and pathogenesis, we performed a comparative proteomic analysis of the conidia and mycelia of an M. anisopliae strain (Ma1291). The analysis used 2-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. We detected 898 ± 37 protein spots in conidia and 1072 ± 24 in mycelia of strain Ma1291. A comparison of the 2 protein-expression profiles indicated that only 28% of protein spots were common to both developmental stages. Finally, we identified 30 proteins (19 from conidia and 11 from mycelia). The identified proteins exclusive to conidia were those involved in protective processes, appressorium formation, and degradation of the host cuticle (protease PR1H). The identified proteins exclusive to mycelia included major proteins participating in biosynthetic and energy metabolism, such as UTP-glucose-1-phosphate uridylyltransferase and heat shock protein 70. This research provides the first proteomic analysis of different developmental stages of M. anisopliae, and the results should facilitate clarification of the molecular basis of these epigenetic variations.

scholarly journals Proteomic Analysis of Mouse Brain Subjected to Spaceflight

2018 ◽  
Author(s):  
Xiao Wen Mao ◽  
Lawrence B. Sandberg ◽  
Daila S. Gridley ◽  
Cliff Herrmann ◽  
Guangyu Zhang ◽  
...  
Keyword(s):  
Protein Expression ◽  
Mouse Brain ◽  
Proteomic Analysis ◽  
Space Shuttle ◽  
Expression Profiles ◽  
Organelle Transport ◽  
Neuronal Structure ◽  
The Central Nervous System ◽  
Kennedy Space ◽  
Protein Expression Profiles

There is evidence that spaceflight poses acute and late risks on the central nervous system. To explore possible mechanisms, the proteomic changes following spaceflight in mouse brain were characterized. Space Shuttle Atlantis (STS-135) was launched at the Kennedy Space Center on a 13-day mission. Within 3–5 hours after landing, brain tissue was collected to evaluate protein expression profiles using quantitative proteomic analysis. Our results showed that there were 26 proteins that were significantly altered after spaceflight in the grey and/or white matter. While there was no overlap between the white and grey matter in terms of individual proteins, there was overlap in terms of function, synaptic plasticity, vesical activity, protein/organelle transport, and metabolism. Our data demonstrate that exposure to the spaceflight environment induces significant changes in protein expression related to neuronal structure and metabolic function. This might lead to a significant impact on brain structural and functional integrity that could affect the outcome of space missions.

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