Proteasome inhibitor lactacystin disturbs the intracellular calcium homeostasis of dopamine neurons in ventral mesencephalic cultures

2007 ◽  
Vol 50 (7-8) ◽  
pp. 959-965 ◽  
Author(s):  
X LI ◽  
D YANG ◽  
L LI ◽  
C PENG ◽  
S CHEN ◽  
...  
Keyword(s):  
Intracellular Calcium ◽  
Calcium Homeostasis ◽  
Proteasome Inhibitor ◽  
Dopamine Neurons ◽  
Intracellular Calcium Homeostasis ◽  
Ventral Mesencephalic

scholarly journals Bortezomib Inhibits Hypoxia-Induced Proliferation by Suppressing Caveolin-1/SOCE/[Ca2+]i Signaling Axis in Human PASMCs

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Chao Wang ◽  
Yuanqi Li ◽  
Lei Xu ◽  
Qiang Zhang ◽  
Gegentuya ◽  
...  
Keyword(s):  
Protein Degradation ◽  
Intracellular Calcium ◽  
Calcium Homeostasis ◽  
Proteasome Inhibitor ◽  
Cell Model ◽  
Protein Synthesis Inhibitor ◽  
Mrna Transcription ◽  
Caveolin 1 ◽  
Intracellular Calcium Homeostasis ◽  
Ubiquitin Proteasome

Background. Previous studies have demonstrated the ubiquitin-proteasome inhibitor bortezomib (BTZ) can effectively alleviate hypoxia-induced pulmonary hypertension (HPH) by suppressing the intracellular calcium homeostasis in pulmonary arterial smooth muscle cells (PASMCs). Further evaluation showed that the antiproliferation roles of BTZ are mainly mediated by inhibition of the intracellular calcium homeostasis. Caveolin-1 belongs to one of the key regulators of the intracellular calcium homeostasis in PASMCs, which can regulate the store-operated calcium entry (SOCE). However, the effects of BTZ on Caveolin-1 remain unclear. Methods. Primarily cultured human PASMCs were used as the cell model. CCK-8 assay was performed to assess the PASMCs proliferation. Western blotting and real-time qPCR were used to detect the mRNA and protein expressions. Fura-2-based fluorescence imaging experiments were used to determine the intracellular calcium concentration ([Ca2+]i). The protein synthesis inhibitor cycloheximide (CHX) was utilized to determine the protein degradation process. Results. Firstly, in cultured human PASMCs, treatment of BTZ for 24 or 60 hours significantly downregulates Caveolin-1 at both mRNA and protein levels. Secondly, in the presence CHX, BTZ treatment also leads to downregulated protein expression and fastened protein degradation of Caveolin-1, indicating that BTZ can promote the Caveolin-1 protein degradation, other than the BTZ on Caveolin-1 mRNA transcription. Then, BTZ significantly attenuates the hypoxia-elevated baseline [Ca2+]i, SOCE, and cell proliferation. Conclusion. We firstly observed that the ubiquitin-proteasome inhibitor BTZ can inhibit the Caveolin-1 expression at both mRNA transcription and protein degradation processes, providing new mechanistic basis of BTZ on PASMC proliferation.

scholarly journals Scn1bdeletion leads to increased tetrodotoxin-sensitive sodium current, altered intracellular calcium homeostasis and arrhythmias in murine hearts

2014 ◽  
Vol 593 (6) ◽  
pp. 1389-1407 ◽  
Author(s):  
Xianming Lin ◽  
Heather O'Malley ◽  
Chunling Chen ◽  
David Auerbach ◽  
Monique Foster ◽  
...  
Keyword(s):  
Intracellular Calcium ◽  
Calcium Homeostasis ◽  
Sodium Current ◽  
Intracellular Calcium Homeostasis

Alterations of Intracellular Calcium Homeostasis and Myocardial Energetics in Acute Adriamycin-Induced Heart Failure

1991 ◽  
Vol 18 (3) ◽  
pp. 437-444 ◽  
Author(s):  
Hideo Kusuoka ◽  
Shiho Futaki ◽  
Yukihiro Koretsune ◽  
Akira Kitabatake ◽  
Hiroyuki Suga ◽  
...  
Keyword(s):  
Heart Failure ◽  
Intracellular Calcium ◽  
Calcium Homeostasis ◽  
Intracellular Calcium Homeostasis ◽  
Myocardial Energetics

scholarly journals Protein kinase CK2 impact on intracellular calcium homeostasis in prostate cancer

2020 ◽  
Vol 470 (1-2) ◽  
pp. 131-143
Author(s):  
Muhammad Afzal ◽  
Betsy T. Kren ◽  
A. Khaliq Naveed ◽  
Janeen H. Trembley ◽  
Khalil Ahmed
Keyword(s):  
Prostate Cancer ◽  
Protein Kinase ◽  
Intracellular Calcium ◽  
Calcium Homeostasis ◽  
Protein Kinase Ck2 ◽  
Intracellular Calcium Homeostasis ◽  
Kinase Ck2

scholarly journals Disturbed intracellular calcium homeostasis in neural tube defects in diabetic embryopathy

2019 ◽  
Vol 514 (3) ◽  
pp. 960-966
Author(s):  
Zhiyong Zhao ◽  
Lixue Cao ◽  
Erick Hernández-Ochoa ◽  
Martin F. Schneider ◽  
E. Albert Reece
Keyword(s):  
Intracellular Calcium ◽  
Neural Tube ◽  
Neural Tube Defects ◽  
Calcium Homeostasis ◽  
Diabetic Embryopathy ◽  
Intracellular Calcium Homeostasis
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