scholarly journals High glucose and diabetes increase the release of [3H]-d-aspartate in retinal cell cultures and in rat retinas

2006 ◽  
Vol 48 (6-7) ◽  
pp. 453-458 ◽  
Author(s):  
Ana R. Santiago ◽  
Tiago S. Pereira ◽  
Manuel J. Garrido ◽  
Armando J. Cristóvão ◽  
Paulo F. Santos ◽  
...  
Keyword(s):  
Cell Cultures ◽  
High Glucose ◽  
Retinal Cell

scholarly journals Elevated Glucose and Interleukin-1β Differentially Affect Retinal Microglial Cell Proliferation

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Filipa I. Baptista ◽  
Célia A. Aveleira ◽  
Áurea F. Castilho ◽  
António F. Ambrósio
Keyword(s):  
Cell Proliferation ◽  
Diabetic Retinopathy ◽  
Cell Cultures ◽  
High Glucose ◽  
Neural Cell ◽  
Microglia Activation ◽  
Retinal Cell ◽  
Diabetic Patients ◽  
Low Grade ◽  
Activated State

Diabetic retinopathy is considered a neurovascular disorder, hyperglycemia being considered the main risk factor for this pathology. Diabetic retinopathy also presents features of a low-grade chronic inflammatory disease, including increased levels of cytokines in the retina, such as interleukin-1 beta (IL-1β). However, how high glucose and IL-1β affect the different retinal cell types remains to be clarified. In retinal neural cell cultures, we found that IL-1β and IL-1RI are present in microglia, macroglia, and neurons. Exposure of retinal neural cell cultures to high glucose upregulated both mRNA and protein levels of IL-1β. High glucose decreased microglial and macroglial cell proliferation, whereas IL-1β increased their proliferation. Interestingly, under high glucose condition, although the number of microglial cells decreased, they showed a less ramified morphology, suggesting a more activated state, as supported by the upregulation of the levels of ED-1, a marker of microglia activation. In conclusion, IL-1β might play a key role in diabetic retinopathy, affecting microglial and macroglial cells and ultimately contributing to neural changes observed in diabetic patients. Particularly, since IL-1β has an important role in retinal microglia activation and proliferation under diabetes, limiting IL-1β-triggered inflammatory processes may provide a new therapeutic strategy to prevent the progression of diabetic retinopathy.

Effects of carbamylcholine on chick embryo aggregate retina cell cultures

1988 ◽  
Vol 46 ◽  
pp. 350-351
Author(s):  
Glenn M. Cohen ◽  
Radharaman Ray
Keyword(s):  
Cell Cultures ◽  
Single Cells ◽  
Retinal Cell ◽  
Cell Aggregates ◽  
High Concentration ◽  
In Ovo ◽  
Sterile Culture ◽  
Retinal Tissue ◽  
Synaptic Junctions ◽  
And Control

Retinal,cell aggregates develop in culture in a pattern similar to the in ovo retina, forming neurites first and then synapses. In the present study, we continuously exposed chick retinal cell aggregates to a high concentration (1 mM) of carbamylcholine (carbachol), an acetylcholine (ACh) analog that resists hydrolysis by acetylcholinesterase (AChE). This situation is similar to organophosphorus anticholinesterase poisoning in which the ACh level is elevated at synaptic junctions due to inhibition of AChE, Our objective was to determine whether continuous carbachol exposure either damaged cholino- ceptive neurites, cell bodies, and synaptic elements of the aggregates or influenced (hastened or retarded) their development.The retinal tissue was isolated aseptically from 11 day embryonic White Leghorn chicks and then enzymatically (trypsin) and mechanically (trituration) dissociated into single cells. After washing the cells by repeated suspension and low (about 200 x G) centrifugation twice, aggregate cell cultures (about l0 cells/culture) were initiated in 1.5 ml medium (BME, GIBCO) in 35 mm sterile culture dishes and maintained as experimental (containing 10-3 M carbachol) and control specimens.

Induction of apoptosis in rat retinal cell cultures by partially fluorinated alkanes

2005 ◽  
Vol 139 (4) ◽  
pp. 737-739 ◽  
Author(s):  
Fiorella Malchiodi-Albedi ◽  
Andrea Matteucci ◽  
Giuseppe Formisano ◽  
Silvia Paradisi ◽  
Giovanna Carnovale-Scalzo ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Retinal Cell ◽  
Induction Of Apoptosis

Mitochondrial Inhibition in Rat Retinal Cell Cultures as a Model of Metabolic Compromise: Mechanisms of Injury and Neuroprotection

2012 ◽  
Vol 53 (8) ◽  
pp. 4897 ◽  
Author(s):  
John P. M. Wood ◽  
Teresa Mammone ◽  
Glyn Chidlow ◽  
Tim Greenwell ◽  
Robert J. Casson
Keyword(s):  
Cell Cultures ◽  
Retinal Cell

scholarly journals Protective effects of tetramethylpyrazine on rat retinal cell cultures

2008 ◽  
Vol 52 (6) ◽  
pp. 1176-1187 ◽  
Author(s):  
Zhikuan Yang ◽  
Qingjiong Zhang ◽  
Jian Ge ◽  
Zhiqun Tan
Keyword(s):  
Cell Cultures ◽  
Retinal Cell ◽  
Protective Effects

Ghrelin inhibits choroid-retinal cell migration, proliferation and in vitro angiogenesis, under a high glucose environment

2015 ◽  
Vol 93 ◽  
pp. n/a-n/a
Author(s):  
A. Rocha De Sousa ◽  
R. Silva-Gomes ◽  
G. Conceição ◽  
A. Moleiro ◽  
C. Santos ◽  
...  
Keyword(s):  
Cell Migration ◽  
High Glucose ◽  
Retinal Cell ◽  
In Vitro Angiogenesis

scholarly journals Immunocytochemical Profiling of Cultured Mouse Primary Retinal Cells

2017 ◽  
Vol 65 (4) ◽  
pp. 223-239 ◽  
Author(s):  
Marina C. Zalis ◽  
Sebastian Johansson ◽  
Ulrica Englund-Johansson
Keyword(s):  
Cell Cultures ◽  
Structural Information ◽  
Expression Profiles ◽  
Retinal Cell ◽  
Research Translation ◽  
Specific Cell ◽  
Retinal Cells ◽  
Cellular Expression

Primary retinal cell cultures and immunocytochemistry are important experimental platforms in ophthalmic research. Translation of retinal cells from their native environment to the in vitro milieu leads to cellular stress, jeopardizing their in vivo phenotype features. Moreover, the specificity and stability of many retinal immunochemical markers are poorly evaluated in retinal cell cultures. Hence, we here evaluated the expression profile of 17 retinal markers, that is, recoverin, rhodopsin, arrestin, Chx10, PKC, DCX, CRALBP, GS, vimentin, TPRV4, RBPMS, Brn3a, β-tubulin III, NeuN, MAP2, GFAP, and synaptophysin. At 7 and 18 days of culture, the marker expression profiles of mouse postnatal retinal cells were compared with their age-matched in vivo retinas. We demonstrate stable in vitro expression of all markers, except for arrestin and CRALBP. Differences in cellular expression and location of some markers were observed, both over time in culture and compared with the age-matched retina. We hypothesize that these differences are likely culture condition dependent. Taken together, we suggest a thorough evaluation of the antibodies in specific culture settings, before extrapolating the in vitro results to an in vivo setting. Moreover, the identification of specific cell types may require a combination of different genes expressed or markers with structural information.

scholarly journals Effect of Substrate Nanotopography and Chemistry on Cell Attachment and Population in Retinal Cell Cultures

2012 ◽  
Vol 102 (3) ◽  
pp. 707a
Author(s):  
Gaelle Piret ◽  
Maria-Thereza Perez ◽  
Christelle Prinz
Keyword(s):  
Cell Cultures ◽  
Cell Attachment ◽  
Retinal Cell
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