Mitochondrial Inhibition in Rat Retinal Cell Cultures as a Model of Metabolic Compromise: Mechanisms of Injury and Neuroprotection

2012 ◽  
Vol 53 (8) ◽  
pp. 4897 ◽  
Author(s):  
John P. M. Wood ◽  
Teresa Mammone ◽  
Glyn Chidlow ◽  
Tim Greenwell ◽  
Robert J. Casson
Keyword(s):  
Cell Cultures ◽  
Retinal Cell

Effects of carbamylcholine on chick embryo aggregate retina cell cultures

1988 ◽  
Vol 46 ◽  
pp. 350-351
Author(s):  
Glenn M. Cohen ◽  
Radharaman Ray
Keyword(s):  
Cell Cultures ◽  
Single Cells ◽  
Retinal Cell ◽  
Cell Aggregates ◽  
High Concentration ◽  
In Ovo ◽  
Sterile Culture ◽  
Retinal Tissue ◽  
Synaptic Junctions ◽  
And Control

Retinal,cell aggregates develop in culture in a pattern similar to the in ovo retina, forming neurites first and then synapses. In the present study, we continuously exposed chick retinal cell aggregates to a high concentration (1 mM) of carbamylcholine (carbachol), an acetylcholine (ACh) analog that resists hydrolysis by acetylcholinesterase (AChE). This situation is similar to organophosphorus anticholinesterase poisoning in which the ACh level is elevated at synaptic junctions due to inhibition of AChE, Our objective was to determine whether continuous carbachol exposure either damaged cholino- ceptive neurites, cell bodies, and synaptic elements of the aggregates or influenced (hastened or retarded) their development.The retinal tissue was isolated aseptically from 11 day embryonic White Leghorn chicks and then enzymatically (trypsin) and mechanically (trituration) dissociated into single cells. After washing the cells by repeated suspension and low (about 200 x G) centrifugation twice, aggregate cell cultures (about l0 cells/culture) were initiated in 1.5 ml medium (BME, GIBCO) in 35 mm sterile culture dishes and maintained as experimental (containing 10-3 M carbachol) and control specimens.

Induction of apoptosis in rat retinal cell cultures by partially fluorinated alkanes

2005 ◽  
Vol 139 (4) ◽  
pp. 737-739 ◽  
Author(s):  
Fiorella Malchiodi-Albedi ◽  
Andrea Matteucci ◽  
Giuseppe Formisano ◽  
Silvia Paradisi ◽  
Giovanna Carnovale-Scalzo ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Retinal Cell ◽  
Induction Of Apoptosis

scholarly journals Protective effects of tetramethylpyrazine on rat retinal cell cultures

2008 ◽  
Vol 52 (6) ◽  
pp. 1176-1187 ◽  
Author(s):  
Zhikuan Yang ◽  
Qingjiong Zhang ◽  
Jian Ge ◽  
Zhiqun Tan
Keyword(s):  
Cell Cultures ◽  
Retinal Cell ◽  
Protective Effects

scholarly journals Immunocytochemical Profiling of Cultured Mouse Primary Retinal Cells

2017 ◽  
Vol 65 (4) ◽  
pp. 223-239 ◽  
Author(s):  
Marina C. Zalis ◽  
Sebastian Johansson ◽  
Ulrica Englund-Johansson
Keyword(s):  
Cell Cultures ◽  
Structural Information ◽  
Expression Profiles ◽  
Retinal Cell ◽  
Research Translation ◽  
Specific Cell ◽  
Retinal Cells ◽  
Cellular Expression

Primary retinal cell cultures and immunocytochemistry are important experimental platforms in ophthalmic research. Translation of retinal cells from their native environment to the in vitro milieu leads to cellular stress, jeopardizing their in vivo phenotype features. Moreover, the specificity and stability of many retinal immunochemical markers are poorly evaluated in retinal cell cultures. Hence, we here evaluated the expression profile of 17 retinal markers, that is, recoverin, rhodopsin, arrestin, Chx10, PKC, DCX, CRALBP, GS, vimentin, TPRV4, RBPMS, Brn3a, β-tubulin III, NeuN, MAP2, GFAP, and synaptophysin. At 7 and 18 days of culture, the marker expression profiles of mouse postnatal retinal cells were compared with their age-matched in vivo retinas. We demonstrate stable in vitro expression of all markers, except for arrestin and CRALBP. Differences in cellular expression and location of some markers were observed, both over time in culture and compared with the age-matched retina. We hypothesize that these differences are likely culture condition dependent. Taken together, we suggest a thorough evaluation of the antibodies in specific culture settings, before extrapolating the in vitro results to an in vivo setting. Moreover, the identification of specific cell types may require a combination of different genes expressed or markers with structural information.

scholarly journals Effect of Substrate Nanotopography and Chemistry on Cell Attachment and Population in Retinal Cell Cultures

2012 ◽  
Vol 102 (3) ◽  
pp. 707a
Author(s):  
Gaelle Piret ◽  
Maria-Thereza Perez ◽  
Christelle Prinz
Keyword(s):  
Cell Cultures ◽  
Cell Attachment ◽  
Retinal Cell

Glutamate Induces Glutathione Efflux Mediated by Glutamate/Aspartate Transporter in Retinal Cell Cultures

2010 ◽  
Vol 36 (3) ◽  
pp. 412-418 ◽  
Author(s):  
T. B. Garcia ◽  
K. R. M. Oliveira ◽  
J. L. M. do Nascimento ◽  
M. E. Crespo-López ◽  
D. L. W. Picanço-Diniz ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Retinal Cell

scholarly journals Coronavirus (JHM) Replication within the Retina: Analysis of Cell Tropism in Mouse Retinal Cell Cultures

Virology ◽  
1993 ◽  
Vol 193 (1) ◽  
pp. 124-137 ◽  
Author(s):  
Yun Wang ◽  
Barbara Detrick ◽  
John J. Hooks
Keyword(s):  
Cell Cultures ◽  
Retinal Cell ◽  
Cell Tropism
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