Effects of cell-free layer formation on NO/O2 bioavailability in small arterioles

2012 ◽  
Vol 83 (2) ◽  
pp. 168-177 ◽  
Author(s):  
Peng Kai Ong ◽  
Seungkwan Cho ◽  
Bumseok Namgung ◽  
Sangho Kim
Keyword(s):  
Layer Formation ◽  
Free Layer ◽  
Cell Free Layer

Cell-Free Layer Formation in Small Arterioles at Pathological Levels of Erythrocyte Aggregation

2011 ◽  
Vol 18 (7) ◽  
pp. 541-551 ◽  
Author(s):  
PENG KAI ONG ◽  
SWATI JAIN ◽  
BUMSEOK NAMGUNG ◽  
YEON I. WOO ◽  
SANGHO KIM
Keyword(s):  
Erythrocyte Aggregation ◽  
Layer Formation ◽  
Free Layer ◽  
Cell Free Layer

scholarly journals Effect of erythrocyte aggregation and flow rate on cell-free layer formation in arterioles

2010 ◽  
Vol 298 (6) ◽  
pp. H1870-H1878 ◽  
Author(s):  
Peng Kai Ong ◽  
Bumseok Namgung ◽  
Paul C. Johnson ◽  
Sangho Kim
Keyword(s):  
Blood Cell ◽  
Flow Rate ◽  
Red Blood Cell ◽  
Cell Aggregation ◽  
Rheological Parameters ◽  
Layer Formation ◽  
Free Layer ◽  
Layer Width ◽  
Red Blood Cell Aggregation ◽  
Cell Free Layer

Formation of a cell-free layer is an important dynamic feature of microcirculatory blood flow, which can be influenced by rheological parameters, such as red blood cell aggregation and flow rate. In this study, we investigate the effect of these two rheological parameters on cell-free layer characteristics in the arterioles (20–60 μm inner diameter). For the first time, we provide here the detailed temporal information of the arteriolar cell-free layer in various rheological conditions to better describe the characteristics of the layer variation. The rat cremaster muscle was used to visualize arteriolar flows, and the extent of aggregation was raised by dextran 500 infusion to levels seen in normal human blood. Our results show that cell-free layer formation in the arterioles is enhanced by a combination of flow reduction and red blood cell aggregation. A positive relation ( P < 0.005) was found between mean cell-free layer widths and their corresponding SDs for all conditions. An analysis of the frequency and magnitudes of cell-free layer variation from their mean value revealed that the layer deviated with significantly larger magnitudes into the red blood cell core after flow reduction and dextran infusion ( P < 0.05). In accordance, the disparity of cell-free layer width distribution found in opposite radial directions from its mean became greater with aggregation in reduced flow conditions. This study shows that the cell-free layer width in arterioles is dependent on both flow rate and red blood cell aggregability, and that the temporal variations in width are asymmetric with a greater excursion into the red blood cell core than toward the vessel wall.

scholarly journals Emergent cell-free layer asymmetry and biased haematocrit partition in a biomimetic vascular network of successive bifurcations

Soft Matter ◽  
2021 ◽  
Author(s):  
Qi Zhou ◽  
Joana Fidalgo ◽  
Miguel Bernabeu ◽  
Mónica S.N. Oliveira ◽  
Timm Krüger
Keyword(s):  
Red Blood Cells ◽  
Human Body ◽  
Blood Cells ◽  
Soft Matter ◽  
Vascular Network ◽  
Free Layer ◽  
Cell Free Layer

Blood is a vital soft matter, and its normal circulation in the human body relies on the distribution of red blood cells (RBCs) at successive bifurcations. Understanding how RBCs are...

scholarly journals Effect of Cell-Free Layer Variation on Arteriolar Wall Shear Stress

2010 ◽  
Vol 39 (1) ◽  
pp. 359-366 ◽  
Author(s):  
Bumseok Namgung ◽  
Peng Kai Ong ◽  
Paul C. Johnson ◽  
Sangho Kim
Keyword(s):  
Shear Stress ◽  
Wall Shear Stress ◽  
Free Layer ◽  
Arteriolar Wall ◽  
Wall Shear ◽  
Cell Free Layer

scholarly journals Visualization and Quantification of the Cell-free Layer in Arterioles of the Rat Cremaster Muscle

10.3791/54550 ◽  
2016 ◽  
Author(s):  
Yan Cheng Ng ◽  
Liam K. Fisher ◽  
Veena Salim ◽  
Sangho Kim ◽  
Bumseok Namgung
Keyword(s):  
Cremaster Muscle ◽  
Free Layer ◽  
Cell Free Layer

Quantitative evaluation of flow dynamics of erythrocytes in microvessels: influence of erythrocyte aggregation

1995 ◽  
Vol 268 (5) ◽  
pp. H1959-H1965 ◽  
Author(s):  
M. Soutani ◽  
Y. Suzuki ◽  
N. Tateishi ◽  
N. Maeda
Keyword(s):  
Flow Resistance ◽  
Circulatory System ◽  
Flow Dynamics ◽  
Erythrocyte Aggregation ◽  
Intensity Change ◽  
Image Analyzer ◽  
Free Layer ◽  
High Shear Rates ◽  
Suspension Viscosity ◽  
Cell Free Layer

Effects of erythrocyte aggregation on the flow dynamics of erythrocytes in microvessels were examined quantitatively by perfusing human erythrocytes suspended in isotonic medium containing various concentrations of dextran (70,400 avg mol wt, Dx-70) into a part of the microvascular bed isolated from rabbit mesentery. Thickness of the marginal cell-free layer was measured with an image analyzer, total flow resistance was determined on the basis of the perfusion pressure-volume flow relationship, and homogeneity of erythrocyte flow was evaluated by the power spectrum obtained by the fast Fourier transform of the light intensity change monitored on single microvessels. With increasing dextran concentration, suspension viscosity of erythrocytes at high shear rates increased linearly and thickness of the cell-free layer increased in a sigmoidal fashion. Flow resistance increased relatively little over the range of dextran concentrations in which the cell-free layer increased most rapidly. Furthermore, the flow pattern of erythrocytes in microvessels became inhomogeneous. In conclusion, the present study shows that Dx-70-induced erythrocyte aggregation results in increased flow resistance in the circulatory system, even through the widening of the cell-free layer tends to reduce the resistance and also results in inhomogeneous flow of erythrocytes in microvessels.

scholarly journals Effects of cell‐free layer width and its variability on wall shear stress in arterioles

2009 ◽  
Vol 23 (S1) ◽  
Author(s):  
BumSeok Namgung ◽  
Peng Kai Ong ◽  
Paul C Johnson ◽  
Sangho Kim
Keyword(s):  
Shear Stress ◽  
Wall Shear Stress ◽  
Free Layer ◽  
Layer Width ◽  
Wall Shear ◽  
Cell Free Layer
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