Is Vasa such a highly specific marker for primordial germ cells? A comparison of VASA and HSP90 proteins expression in young chicken embryos

Morphologie ◽  
2020 ◽  
Vol 104 (344) ◽  
pp. 20-26 ◽  
Author(s):  
M. Lejong ◽  
M. Choa-Duterre ◽  
N. Vanmuylder ◽  
S. Louryan
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Specific Marker ◽  
Chicken Embryos ◽  
Young Chicken

scholarly journals In Vivo Transfer of Foreign DNA into Primordial Germ Cells (PGCs) of Chicken Embryos

1999 ◽  
Vol 12 (4) ◽  
pp. 520-524 ◽  
Author(s):  
K. Eguma ◽  
T. Soh ◽  
M. Hattori ◽  
N. Fujihara
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Chicken Embryos ◽  
Foreign Dna

Increased proportion of donor primordial germ cells in chimeric gonads by sterilisation of recipient embryos using busulfan sustained-release emulsion in chickens

2008 ◽  
Vol 20 (8) ◽  
pp. 900 ◽  
Author(s):  
Yoshiaki Nakamura ◽  
Yasuhiro Yamamoto ◽  
Fumitake Usui ◽  
Yusuke Atsumi ◽  
Yohei Ito ◽  
...  
Keyword(s):  
Sustained Release ◽  
Germ Cell ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Primordial Germ Cell ◽  
Sesame Oil ◽  
Chicken Embryos ◽  
Whole Mount ◽  
Phosphate Buffered Saline ◽  
Transfer Study

The aim of the present study was to improve the efficiency of endogenous primordial germ cell (PGC) depletion and to increase the ratio of donor PGCs in the gonads of recipient chicken embryos. A sustained-release emulsion was prepared by emulsifying equal amounts of Ca2+- and Mg2+-free phosphate-buffered saline containing 10% busulfan solubilised in N,N-dimethylformamide and sesame oil, using a filter. Then, 75 μg per 50 μL busulfan sustained-release emulsion was injected into the yolk. To determine the depletion and repopulation of PGCs in the gonads after 6 days incubation, whole-mount immunostaining was performed. The busulfan sustained-release emulsion significantly reduced the number of endogenous PGCs compared with control (P < 0.05). Moreover, the busulfan sustained-release emulsion significantly depleted endogenous PGCs compared with other previously reported busulfan delivery systems (P < 0.05), but with less variation, suggesting that the sustained-release emulsion delivered a consistent amount of busulfan to the developing chicken embryos. The PGC transfer study showed that the proportion of donor PGCs in the gonads of busulfan sustained-release emulsion-treated embryos after 6 days incubation increased 28-fold compared with control. In conclusion, the results demonstrate that exogenous PGCs are capable of migrating and settling in gonads from which endogenous PGCs have been removed using a busulfan sustained-release emulsion.

scholarly journals Knockdown of DEAD-box helicase 4 (DDX4) decreases the number of germ cells in male and female chicken embryonic gonads

2019 ◽  
Vol 31 (5) ◽  
pp. 847
Author(s):  
Nana Aduma ◽  
Hiroe Izumi ◽  
Shusei Mizushima ◽  
Asato Kuroiwa
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Retroviral Vectors ◽  
Ovary Development ◽  
Aromatase Expression ◽  
Male And Female ◽  
Chicken Embryos ◽  
Dead Box ◽  
Related Transcription Factor ◽  
Cytochrome P450 Family

DEAD-box helicase 4 (DDX4; also known as vasa) is essential for the proper formation and maintenance of germ cells. Although DDX4 is conserved in a variety of vertebrates and invertebrates, its roles differ between species. This study investigated the function of DDX4 in chicken embryos by knocking down its expression using retroviral vectors that encoded DDX4-targeting microRNAs. DDX4 was effectively depleted invitro and invivo via this approach. Male and female gonads of DDX4-knockdown embryos contained a decreased number of primordial germ cells, indicating that DDX4 is essential to maintain a normal level of these cells in chicken embryos of both sexes. Expression of doublesex and mab-3 related transcription factor 1 (DMRT1) and sex determining region Y-box 9 (SOX9), which are involved in testis determination and differentiation, was normal in male gonads of DDX4-knockdown embryos. In contrast, expression of cytochrome P450 family 19 subfamily A member 1 (CYP19A1), which encodes aromatase and is essential for ovary development, was significantly decreased in female gonads of DDX4-knockdown embryos. Expression of forkhead box L2 (FOXL2), which plays an important role in ovary differentiation, was also slightly reduced in DDX4-knockdown embryos, but not significantly. Based on several pieces of evidence FOXL2 was hypothesised to regulate aromatase expression. The results of this study indicate that aromatase expression is also regulated by several additional pathways.

scholarly journals Intense Expression of GFP Gene in Gonads of Chicken Embryos by Transfecting Circulating Primordial Germ Cells in vitro and in vivo

2007 ◽  
Vol 44 (4) ◽  
pp. 416-425 ◽  
Author(s):  
Mitsuru Naito ◽  
Takeo Minematsu ◽  
Takashi Harumi ◽  
Takashi Kuwana
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Chicken Embryos ◽  
Gfp Gene

scholarly journals Foreign Gene Expression in the Gonads of Chimaeric Chicken Embryos by Transfer of Primordial Germ Cells Transfected in vitro by Lipofection for 24 Hours

2000 ◽  
Vol 71 (3) ◽  
pp. 308-311
Author(s):  
Mitsuru NAITO ◽  
Yuko MATSUBARA ◽  
Takashi HARUMI ◽  
Takahiro TAGAMI ◽  
Michiharu SAKURAI ◽  
...  
Keyword(s):  
Gene Expression ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Foreign Gene ◽  
Foreign Gene Expression ◽  
Chicken Embryos

Laser irradiation of the chick embryo germinal crescent

Development ◽  
1971 ◽  
Vol 26 (1) ◽  
pp. 31-36
Author(s):  
Martha Fearon Mims ◽  
Robert Gilmore McKinnell
Keyword(s):  
Chick Embryo ◽  
Laser Irradiation ◽  
Germ Cells ◽  
Ruby Laser ◽  
Primordial Germ Cells ◽  
Chicken Embryos ◽  
Somite Stage ◽  
Multiple Pulses

The germinal crescent of head-fold and one-somite-stage chicken embryos was irradiated with multiple pulses of a microbeam ruby laser. Primordial germ cells were not detected in the gonad primordium of six laser-irradiated 5-day embryos; ten laser-irradiated embryos had varying numbers of primordial germ cells. Ten control embryos had gonad primordia populated with many primordial germ cells.

scholarly journals Concentration and total number of circulating primordial germ cells in Green-legged Partridgelike chicken embryos

2021 ◽  
Vol 100 (1) ◽  
pp. 319-324
Author(s):  
Agata Szczerba ◽  
Takashi Kuwana ◽  
Marek Bednarczyk
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Chicken Embryos

150 THE DISTRIBUTION PATTERN OF PRIMORDIAL GERM CELLS IN EARLY CHICK EMBRYOS

2007 ◽  
Vol 19 (1) ◽  
pp. 192 ◽  
Author(s):  
Y. Nakamura ◽  
Y. Yamamoto ◽  
F. Usui ◽  
T. Ono ◽  
K. Takeda ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Central Zone ◽  
Staging System ◽  
Vascular Network ◽  
Chick Embryos ◽  
Chicken Embryos ◽  
The Future ◽  
The Mean

In all vertebrates, primordial germ cells (PGCs) appear during early stages of development in extragonadal sites, then they migrate to the gonad and give rise to ova or spermatozoa. Unlike in other species, however, in avian and reptile embryos, PGCs use the vascular system as a vehicle to transport them to the future gonadal region where they leave the blood vessels. The present study was carried out to know the details of this unique migration pathway and the proliferation of endogenous PGCs in chicken embryos. Whole of the chicken embryos during stages X [Roman numerals refer to the staging system of Eyal-Giladi and Kochav (1976 Dev. Biol. 49, 321–327) to 17 (Arabic numerals refer to the staging system of Hamburger and Hamilton (1951 J. Morphol. 88, 49-82))] or embryonic blood during stages 12 to 17 were immunohistochemical stained using specific antibody raised against chicken vasa homolog (CVH), which could be recognized as a marker for chicken PGCs. The distribution patterns and populations of PGCs in embryos were observed under a stereomicroscope. The numbers of PGCs were presented mean and standard deviation (mean � SD). Anti-CVH staining revealed the distribution and population of chicken PGCs in early chick embryos. PGCs existed mainly in the area pellucida and concentrated in the central zone at stage X. The mean number of PGCs per embryo at this stage was 130.4 � 31.9. With the formation of primitive streak, PGCs were carried anteriorly to the edge of the blastoderm. The PGCs scattered anteriorly began to concentrate to the anterior point of the head on the dorsal side of stage 10 embryos. The average number of PGCs per embryo at stage 10 was 439.3 � 93.6. The mean numbers of PGCs per embryo during stages X to 10 increased gradually as development progressed to stage 10. We found the entrance point of PGCs from anterior edge of the blastoderm to the vascular network during stages 10 to 11. In the blood, PGCs could be detected from all of the samples during stages 12 to 17. In contrast, no PGC was recognized in the future gonadal region before stage 14, and then they began to appear in the same region at stage 15. The mean numbers of PGCs that located in the future gonadal region during stages 15 to 17 increased intensively and were 97.3 � 57.3, 200.3 � 113.5, and 327.6 � 102.4, respectively. Interestingly, the numbers of PGCs within future gonadal region during stages 15 to 17 were consistently and significantly different (P &lt; 0.05) between the left and right side of the region. The results suggest that chicken PGCs move from extraembryonic area to the vascular network during stages 10 to 11, circulate in the blood stream, and finally, they begin to leave the blood vessels actively and migrate to the future gonadal region at stage 15.

Sign in / Sign up

Export Citation Format

Share Document