scholarly journals Homology Requirements and Competition between Gene Conversion and Break-Induced Replication during Double-Strand Break Repair

2017 ◽  
Vol 65 (3) ◽  
pp. 515-526.e3 ◽  
Author(s):  
Anuja Mehta ◽  
Annette Beach ◽  
James E. Haber
Keyword(s):  
Gene Conversion ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Break Repair ◽  
Break Induced Replication

scholarly journals Mre11 and Ku regulation of double-strand break repair by gene conversion and break-induced replication

DNA Repair ◽  
2007 ◽  
Vol 6 (6) ◽  
pp. 797-808 ◽  
Author(s):  
Sanchita Krishna ◽  
Brant M. Wagener ◽  
Hui Ping Liu ◽  
Yi-Chen Lo ◽  
Rosa Sterk ◽  
...  
Keyword(s):  
Gene Conversion ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Break Repair ◽  
Break Induced Replication

The Effect of Heterologous Insertions on Gene Conversion in Mitotically Dividing Cells in Drosophila melanogaster

Genetics ◽  
2002 ◽  
Vol 161 (1) ◽  
pp. 249-258
Author(s):  
Angela M Coveny ◽  
Tammy Dray ◽  
Gregory B Gloor
Keyword(s):  
Drosophila Melanogaster ◽  
Gene Conversion ◽  
Strand Break ◽  
Double Strand Break ◽  
P Element ◽  
Double Strand Break Repair ◽  
Break Site ◽  
In Trans ◽  
Break Repair ◽  
In Cis

Abstract We examined the influence that heterologous sequences of different sizes have on the frequency of double-strand-break repair by gene conversion in Drosophila melanogaster. We induced a double-strand break on one X chromosome in female flies by P-element excision. These flies contained heterologous insertions of various sizes located 238 bp from the break site in cis or in trans to the break, or both. We observed a significant decrease in double-strand-break repair with large heterologous insertions located either in cis or in trans to the break. Reestablishing the homology by including the same heterologous sequence in cis and in trans to the double-strand break restored the frequency of gene conversion to wild-type levels. In one instance, an allelic nonhomologous insertion completely abolished repair by homologous recombination. The results show that the repair of a double-strand break by gene conversion requires chromosome pairing in the local region of the double-strand break.

scholarly journals Test of the double-strand-break repair model of recombination in Xenopus laevis oocytes.

1992 ◽  
Vol 12 (1) ◽  
pp. 112-119 ◽  
Author(s):  
S J Jeong-Yu ◽  
D Carroll
Keyword(s):  
Xenopus Laevis ◽  
Gene Conversion ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Xenopus Laevis Oocytes ◽  
Direct Test ◽  
Low Levels ◽  
Break Repair ◽  
Dna Substrate

A direct test was made of predictions of the double-strand-break repair (DSBR) model of recombination in Xenopus laevis oocytes. The DNA substrate injected into oocytes had two directly repeated copies of a 1.25-kb sequence and was cleaved within one of them. Different products were expected to result from concerted, conservative events, as predicted by the DSBR model, and from nonconservative events. Only very low levels of recombination products, both conservative and nonconservative, were observed. When individual, apparent DSBR products were cloned and characterized, it emerged that the majority of them had arisen by nonconservative recombination through short, terminal homologies and not from the gene conversion events predicted for DSBR. Two cloned products among 44 tested corresponded to the predications of the DSBR model, but these could also have been generated by other processes. The most efficient recombination events in oocytes are nonconservative and are based on long, terminal homologous overlaps; when these are not available, short, imperfect overlaps support a lower level of nonconservative recombination; genuine, conservative DSBR events occur rarely, if at all.

Test of the double-strand-break repair model of recombination in Xenopus laevis oocytes

1992 ◽  
Vol 12 (1) ◽  
pp. 112-119
Author(s):  
S J Jeong-Yu ◽  
D Carroll
Keyword(s):  
Xenopus Laevis ◽  
Gene Conversion ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Xenopus Laevis Oocytes ◽  
Direct Test ◽  
Low Levels ◽  
Break Repair ◽  
Dna Substrate

A direct test was made of predictions of the double-strand-break repair (DSBR) model of recombination in Xenopus laevis oocytes. The DNA substrate injected into oocytes had two directly repeated copies of a 1.25-kb sequence and was cleaved within one of them. Different products were expected to result from concerted, conservative events, as predicted by the DSBR model, and from nonconservative events. Only very low levels of recombination products, both conservative and nonconservative, were observed. When individual, apparent DSBR products were cloned and characterized, it emerged that the majority of them had arisen by nonconservative recombination through short, terminal homologies and not from the gene conversion events predicted for DSBR. Two cloned products among 44 tested corresponded to the predications of the DSBR model, but these could also have been generated by other processes. The most efficient recombination events in oocytes are nonconservative and are based on long, terminal homologous overlaps; when these are not available, short, imperfect overlaps support a lower level of nonconservative recombination; genuine, conservative DSBR events occur rarely, if at all.

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