scholarly journals Strand Invasion Structures in the Inverted Repeat of Candida albicans Mitochondrial DNA Reveal a Role for Homologous Recombination in Replication

2010 ◽  
Vol 39 (6) ◽  
pp. 851-861 ◽  
Author(s):  
Joachim M. Gerhold ◽  
Anu Aun ◽  
Tiina Sedman ◽  
Priit Jõers ◽  
Juhan Sedman
Keyword(s):  
Mitochondrial Dna ◽  
Candida Albicans ◽  
Homologous Recombination ◽  
Inverted Repeat ◽  
Strand Invasion

scholarly journals Formation of Large Palindromic DNA by Homologous Recombination of Short Inverted Repeat Sequences in Saccharomyces cerevisiae

Genetics ◽  
2002 ◽  
Vol 161 (3) ◽  
pp. 1065-1075
Author(s):  
David K Butler ◽  
David Gillespie ◽  
Brandi Steele
Keyword(s):  
Homologous Recombination ◽  
Inverted Repeat ◽  
Excision Repair ◽  
Strand Break ◽  
Double Strand Break ◽  
Repeat Sequence ◽  
Inverted Repeat Sequence ◽  
Repeat Sequences ◽  
Dna Double Strand Break ◽  
Intermolecular Reaction

Abstract Large DNA palindromes form sporadically in many eukaryotic and prokaryotic genomes and are often associated with amplified genes. The presence of a short inverted repeat sequence near a DNA double-strand break has been implicated in the formation of large palindromes in a variety of organisms. Previously we have established that in Saccharomyces cerevisae a linear DNA palindrome is efficiently formed from a single-copy circular plasmid when a DNA double-strand break is introduced next to a short inverted repeat sequence. In this study we address whether the linear palindromes form by an intermolecular reaction (that is, a reaction between two identical fragments in a head-to-head arrangement) or by an unusual intramolecular reaction, as it apparently does in other examples of palindrome formation. Our evidence supports a model in which palindromes are primarily formed by an intermolecular reaction involving homologous recombination of short inverted repeat sequences. We have also extended our investigation into the requirement for DNA double-strand break repair genes in palindrome formation. We have found that a deletion of the RAD52 gene significantly reduces palindrome formation by intermolecular recombination and that deletions of two other genes in the RAD52-epistasis group (RAD51 and MRE11) have little or no effect on palindrome formation. In addition, palindrome formation is dramatically reduced by a deletion of the nucleotide excision repair gene RAD1.

scholarly journals Repairing a double–strand chromosome break by homologous recombination: revisiting Robin Holliday's model

2004 ◽  
Vol 359 (1441) ◽  
pp. 79-86 ◽  
Author(s):  
James E. Haber ◽  
Gregorz Ira ◽  
Anna Malkova ◽  
Neal Sugawara
Keyword(s):  
Homologous Recombination ◽  
Repair Process ◽  
Strand Break ◽  
Holliday Junctions ◽  
Chromosome Break ◽  
Invasion Mechanism ◽  
Strand Invasion ◽  
Strand Annealing ◽  
Break Induced Replication ◽  
Mutant Cells

Since the pioneering model for homologous recombination proposed by Robin Holliday in 1964, there has been great progress in understanding how recombination occurs at a molecular level. In the budding yeast Saccharomyces cerevisiae , one can follow recombination by physically monitoring DNA after the synchronous induction of a double–strand break (DSB) in both wild–type and mutant cells. A particularly well–studied system has been the switching of yeast mating–type ( MAT ) genes, where a DSB can be induced synchronously by expression of the site–specific HO endonuclease. Similar studies can be performed in meiotic cells, where DSBs are created by the Spo11 nuclease. There appear to be at least two competing mechanisms of homologous recombination: a synthesis–dependent strand annealing pathway leading to noncrossovers and a two–end strand invasion mechanism leading to formation and resolution of Holliday junctions (HJs), leading to crossovers. The establishment of a modified replication fork during DSB repair links gene conversion to another important repair process, break–induced replication. Despite recent revelations, almost 40 years after Holliday's model was published, the essential ideas he proposed of strand invasion and heteroduplex DNA formation, the formation and resolution of HJs, and mismatch repair, remain the basis of our thinking.

scholarly journals End-resection at DNA double-strand breaks in the three domains of life

2013 ◽  
Vol 41 (1) ◽  
pp. 314-320 ◽  
Author(s):  
John K. Blackwood ◽  
Neil J. Rzechorzek ◽  
Sian M. Bray ◽  
Joseph D. Maman ◽  
Luca Pellegrini ◽  
...  
Keyword(s):  
Dna Repair ◽  
Homologous Recombination ◽  
Strand Break ◽  
Double Strand Breaks ◽  
Dna Double Strand Breaks ◽  
Strand Breaks ◽  
Homologous Chromosomes ◽  
Domains Of Life ◽  
Strand Invasion ◽  
End Resection

During DNA repair by HR (homologous recombination), the ends of a DNA DSB (double-strand break) must be resected to generate single-stranded tails, which are required for strand invasion and exchange with homologous chromosomes. This 5′–3′ end-resection of the DNA duplex is an essential process, conserved across all three domains of life: the bacteria, eukaryota and archaea. In the present review, we examine the numerous and redundant helicase and nuclease systems that function as the enzymatic analogues for this crucial process in the three major phylogenetic divisions.

A mitochondrial retroplasmid integrates into mitochondrial DNA by a novel mechanism involving the synthesis of a hybrid cDNA and homologous recombination

1994 ◽  
Vol 14 (10) ◽  
pp. 6419-6432
Author(s):  
C C Chiang ◽  
J C Kennell ◽  
L A Wanner ◽  
A M Lambowitz
Keyword(s):  
Mitochondrial Dna ◽  
Homologous Recombination ◽  
Rrna Gene ◽  
Template Switching ◽  
Transcript Analysis ◽  
Wild Type ◽  
Mitochondrial Trna ◽  
Integration Mechanism ◽  
Circular Dnas ◽  
Simple Integration

The Mauriceville and Varkud mitochondrial plasmids of Neurospora spp. are closely related, small circular DNAs that propagate via an RNA intermediate and reverse transcription. Although the plasmids ordinarily replicate autonomously, they can also integrate into mitochondrial DNA (mtDNA), yielding defective mtDNAs that in some cases cause senescence. To investigate the integration mechanism, we analyzed four cases in which the Varkud plasmid integrated into the mitochondrial small rRNA gene, three in wild-type subcultures and one in a senescent mutant. Our analysis suggests that the integrations occurred by the plasmid reverse transcriptase template switching between the plasmid transcript and internal sequences in the mitochondrial small rRNA to yield hybrid cDNAs that circularized and recombined homologously with the mtDNA. The integrated plasmid sequences are transcribed, presumably from the mitochondrial small rRNA promoters, resulting in hybrid RNAs containing the 5' segment of the mitochondrial small rRNA linked head-to-tail to the full-length plasmid transcript. Analysis of additional senescent mutants revealed three cases in which the plasmid used the same mechanism to integrate at other locations in the mtDNA. In these cases, circular variant plasmids that had incorporated a mitochondrial tRNA or tRNA-like sequence by template switching integrated by homologous recombination at the site of the corresponding tRNA or tRNA-like sequence in mtDNA. This simple integration mechanism involving template switching to generate a hybrid cDNA that integrates homologously could have been used by primitive retroelements prior to the acquisition of a specialized integration machinery.

scholarly journals The Mitochondrial DNA (mtDNA)-Associated Protein SWIB5 Influences mtDNA Architecture and Homologous Recombination

2017 ◽  
pp. tpc.00899.2016 ◽  
Author(s):  
Jonas Blomme ◽  
Olivier Van Aken ◽  
Jelle Van Leene ◽  
Teddy Jégu ◽  
Riet Maria De Rycke ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Homologous Recombination

scholarly journals Partner Choice in Spontaneous Mitotic Recombination in Wild Type and Homologous Recombination Mutants of Candida albicans

2019 ◽  
Vol 9 (11) ◽  
pp. 3631-3644
Author(s):  
Alberto Bellido ◽  
Toni Ciudad ◽  
Belén Hermosa ◽  
Encarnación Andaluz ◽  
Anja Forche ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Homologous Recombination ◽  
Mitotic Recombination ◽  
Partner Choice ◽  
Wild Type

scholarly journals Erratum: Homologous recombination in Candida albicans: role of CaRad52p in DNA repair, integration of linear DNA fragments and telomere length

2005 ◽  
Vol 56 (5) ◽  
pp. 1396-1396 ◽  
Author(s):  
Toni Ciudad ◽  
Encarnación Andaluz ◽  
Olga Steinberg-Neifach ◽  
Neal F. Lue ◽  
Neil A. R. Gow ◽  
...  
Keyword(s):  
Dna Repair ◽  
Candida Albicans ◽  
Homologous Recombination ◽  
Telomere Length ◽  
Dna Fragments ◽  
Linear Dna
Sign in / Sign up

Export Citation Format

Share Document