scholarly journals 08-P016 Maintenance of the orientation of polarity in Drosophila larval brain neuroblasts

2009 ◽  
Vol 126 ◽  
pp. S148
Author(s):  
Jens Januschke ◽  
Cayetano Gonzalez
Keyword(s):  
Larval Brain

scholarly journals Non3 is an essential Drosophila gene required for proper nucleolus assembly

2019 ◽  
Vol 23 (2) ◽  
pp. 190-198
Author(s):  
E. N. Andreyeva ◽  
A. A. Ogienko ◽  
A. A. Yushkova ◽  
J. V. Popova ◽  
G. A. Pavlova ◽  
...  
Keyword(s):  
Ribosome Biogenesis ◽  
Large Deletion ◽  
Protein Product ◽  
P Element ◽  
Post Translational Modification ◽  
Allelic Series ◽  
Larval Brain ◽  
Functional Studies ◽  
Mild Reduction

The nucleolus is a dynamic non-membrane-bound nuclear organelle, which plays key roles not only in ribosome biogenesis but also in many other cellular processes. Consistent with its multiple functions, the nucleolus has been implicated in many human diseases, including cancer and degenerative pathologies of the nervous system and heart. Here, we report the characterization of the Drosophila Non3 (Novel nucleolar protein 3) gene, which encodes a protein homologous to the human Brix domain-containing Rpf2 that has been shown to control ribosomal RNA (rRNA) processing. We used imprecise P-element excision to generate four new mutant alleles in the Non3 gene. Complementation and phenotypic analyses showed that these Non3 mutations can be arranged in an allelic series that includes both viable and lethal alleles. The strongest lethal allele (Non3∆600) is a genetically null allele that carries a large deletion of the gene and exhibits early lethality when homozygous. Flies heterozygous for Non3∆600 occasionally exhibit a mild reduction in the bristle size, but develop normally and are fertile. However, heteroallelic combinations of viable Non3 mutations (Non3197, Non3310 and Non3259) display a Minute-like phenotype, consisting in delayed development and short and thin bristles, suggesting that they are defective in ribosome biogenesis. We also demonstrate that the Non3 protein localizes to the nucleolus of larval brain cells and it is required for proper nucleolar localization of Fibrillarin, a protein important for post-translational modification and processing of rRNAs. In summary, we generated a number of genetic and biochemical tools that were exploited for an initial characterization of Non3, and will be instrumental for future functional studies on this gene and its protein product.

scholarly journals Subdivisions of the adult zebrafish pallium based on molecular marker analysis

F1000Research ◽  
2015 ◽  
Vol 3 ◽  
pp. 308 ◽  
Author(s):  
Julia Ganz ◽  
Volker Kroehne ◽  
Dorian Freudenreich ◽  
Anja Machate ◽  
Michaela Geffarth ◽  
...  
Keyword(s):  
Gene Expression ◽  
Functional Data ◽  
Hippocampal Formation ◽  
Gene Expression Pattern ◽  
Structural Diversity ◽  
Marker Genes ◽  
Expression Data ◽  
Adult Zebrafish ◽  
Larval Brain ◽  
Molecular Marker Analysis

Background: The telencephalon shows a remarkable structural diversity among vertebrates. In particular, the everted telencephalon of ray-finned fishes has a markedly different morphology compared to the evaginated telencephalon of all other vertebrates. This difference in development has hampered the comparison between different areas of the pallium of ray-finned fishes and the pallial nuclei of all other vertebrates. Various models of homology between pallial subdivisions in ray-finned fishes and the pallial nuclei in tetrapods have been proposed based on connectional, neurochemical, gene expression and functional data. However, no consensus has been reached so far. In recent years, the analysis of conserved developmental marker genes has assisted the identification of homologies for different parts of the telencephalon among several tetrapod species.Results: We have investigated the gene expression pattern of conserved marker genes in the adult zebrafish (Danio rerio) pallium to identify pallial subdivisions and their homology to pallial nuclei in tetrapods. Combinatorial expression analysis of ascl1a, eomesa, emx1, emx2, emx3, and Prox1 identifies four main divisions in the adult zebrafish pallium. Within these subdivisions, we propose that Dm is homologous to the pallial amygdala in tetrapods and that the dorsal subdivision of Dl is homologous to part of the hippocampal formation in mouse. We have complemented this analysis be examining the gene expression of emx1, emx2 and emx3 in the zebrafish larval brain.Conclusions: Based on our gene expression data, we propose a new model of subdivisions in the adult zebrafish pallium and their putative homologies to pallial nuclei in tetrapods. Pallial nuclei control sensory, motor, and cognitive functions, like memory, learning and emotion. The identification of pallial subdivisions in the adult zebrafish and their homologies to pallial nuclei in tetrapods will contribute to the use of the zebrafish system as a model for neurobiological research and human neurodegenerative diseases.

scholarly journals Amalgam regulates the receptor tyrosine kinase pathway through Sprouty in glial cell development in the Drosophila larval brain

2020 ◽  
Vol 133 (19) ◽  
pp. jcs250837
Author(s):  
Majd M. Ariss ◽  
Alexander R. Terry ◽  
Abul B. M. M. K. Islam ◽  
Nissim Hay ◽  
Maxim V. Frolov
Keyword(s):  
Cell Proliferation ◽  
Tyrosine Kinase ◽  
Glial Cell ◽  
Receptor Tyrosine Kinase ◽  
Cell Development ◽  
Negative Regulator ◽  
Larval Brain ◽  
Adhesion Protein ◽  
Type Composition ◽  
Signaling Activation

ABSTRACTThe receptor tyrosine kinase (RTK) pathway plays an essential role in development and disease by controlling cell proliferation and differentiation. Here, we profile the Drosophila larval brain by single-cell RNA-sequencing and identify Amalgam (Ama), which encodes a cell adhesion protein of the immunoglobulin IgLON family, as regulating the RTK pathway activity during glial cell development. Depletion of Ama reduces cell proliferation, affects glial cell type composition and disrupts the blood–brain barrier (BBB), which leads to hemocyte infiltration and neuronal death. We show that Ama depletion lowers RTK activity by upregulating Sprouty (Sty), a negative regulator of the RTK pathway. Knockdown of Ama blocks oncogenic RTK signaling activation in the Drosophila glioma model and halts malignant transformation. Finally, knockdown of a human ortholog of Ama, LSAMP, results in upregulation of SPROUTY2 in glioblastoma cell lines, suggesting that the relationship between Ama and Sty is conserved.

Immunofluorescent Staining of Drosophila Larval Brain Tissue

2010 ◽  
Vol 2010 (7) ◽  
pp. pdb.prot5460-pdb.prot5460 ◽  
Author(s):  
A. L. Daul ◽  
H. Komori ◽  
C.-Y. Lee
Keyword(s):  
Brain Tissue ◽  
Immunofluorescent Staining ◽  
Larval Brain

scholarly journals A Role for Blind DN2 Clock Neurons in Temperature Entrainment of the Drosophila Larval Brain

2009 ◽  
Vol 29 (26) ◽  
pp. 8312-8320 ◽  
Author(s):  
M. Picot ◽  
A. Klarsfeld ◽  
E. Chelot ◽  
S. Malpel ◽  
F. Rouyer
Keyword(s):  
Larval Brain ◽  
Temperature Entrainment
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