Immunofluorescent Staining of Drosophila Larval Brain Tissue

2010 ◽  
Vol 2010 (7) ◽  
pp. pdb.prot5460-pdb.prot5460 ◽  
Author(s):  
A. L. Daul ◽  
H. Komori ◽  
C.-Y. Lee
Keyword(s):  
Brain Tissue ◽  
Immunofluorescent Staining ◽  
Larval Brain

Studies on the Binding of Proteins to the Human Platelet Surface: Relation to Platelet Activation

1985 ◽  
Vol 53 (03) ◽  
pp. 360-365 ◽  
Author(s):  
Gerhard K M Endresen ◽  
Øystein Førre
Keyword(s):  
Platelet Aggregation ◽  
Lupus Erythematosus ◽  
Immune Complexes ◽  
Immunofluorescent Staining ◽  
Induce Platelet Aggregation ◽  
Platelet Surface ◽  
Platelet Aggregometry ◽  
Surface Staining ◽  
Alpha1 Antitrypsin ◽  
Induced Aggregation

SummarySeveral antibody fractions and sera from patients with rheumatoid arthritis, systemic lupus erythematosus and chronic idiopathic thrombocytopenic purpura were examined for their ability to bind to normal platelets using immunofluorescent staining techniques. Platelet aggregometry was used to study the activating capacity of the samples.Both C1q, C1s, C1 inactivator, fibrinogen, factor VIII-related antigen, alpha1-acid glycoprotein, alpha1-antitrypsin, beta2-micro- globulin and isoantigens A and B, as well as fibronectin and plasminogen were found on the platelet surface. Only antibodies to C1q, C1s and beta2-microglobulin were able to induce platelet aggregation. Sera containing immune complexes or platelet autoantibodies revealed positive surface staining for IgG, or for IgG and IgM. These sera also induced aggregation of platelets. Sera not containing immune complexes or autoantibodies gave negative staining and aggregation results. Thus, only some of the ligand receptor interactions were able to induce platelet aggregation.

Effects of therapeutic and toxic phenobarbital doses on brain tissue caspase 3-activity in the neonatal rat

2006 ◽  
Vol 37 (03) ◽  
Author(s):  
R Husain ◽  
HS Fink ◽  
K Lang ◽  
B Merkle ◽  
R Bauer ◽  
...  
Keyword(s):  
Brain Tissue ◽  
Caspase 3 ◽  
Neonatal Rat

Effects of Hormone Replacement Therapy on Plasma and Tissue Fibrinolytic Activity in a Rat Model of Surgically Induced Menopause

2014 ◽  
Vol 37 (2) ◽  
pp. 85 ◽  
Author(s):  
Ata Topcuoglu ◽  
Mustafa Albayrak ◽  
Hayriye Erman ◽  
Huriye Balci ◽  
Mesut Karakus ◽  
...  
Keyword(s):  
Hormone Replacement Therapy ◽  
Oral Administration ◽  
Replacement Therapy ◽  
Plasminogen Activator ◽  
Brain Tissue ◽  
Fibrinolytic Activity ◽  
Hormone Replacement ◽  
17Β Estradiol ◽  
Surgically Induced ◽  
Pai 1

Purpose: The purpose of this study was to analyze the effects of estrogen deficiency and hormone replacement therapy (HRT) on fibrinolytic activity in a rat mode of surgically-induced menopause. Methods: Twelve-week-old, sexually mature female Sprague-Dawley rats, each weighing 200–250 g, were randomly divided into four groups: (1) sham-operated group, (2) ovariectomy group, (3) ovariectomy group followed by oral administration of daily 17β-estradiol (0.02 mg/kg/day) (E2) + norethisterone acetate (0.01 mg/kg/day), and (4) ovariectomy group followed by oral administration of daily 17β-estradiol (0.01 mg/kg/day) + drospirenone (0.02 mg/kg/day). Tissue plasminogen activator (tPA) antigen, plasminogen activator inhibitor-1 (PAI-1) antigen, and PAI-1/tPA levels were measured as markers of fibrinolysis in plasma and liver and brain tissue. Results: Compared with sham-operated rats, ovariectomized rats showed higher levels of fibrinolytic activity; however, the increased fibrinolytic activity in plasma and liver tissue was significantly reduced by HRT regimens. No change was observed in the levels of fibrinolytic activity in brain tissue. Conclusions: HRT showed beneficial effects by decreasing fibrinolytic activity related to surgically-induced menopause. Short-term HRT treatment was associated with a shift in the procoagulant-anticoagulant balance toward a procoagulant state.

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