scholarly journals 06-P006 Use of stem cells to identify novel signaling pathways and candidate genes associated with Non-Syndromic Cleft Lip and Palate (NSCL/P)

2009 ◽  
Vol 126 ◽  
pp. S121-S122
Author(s):  
Daniela F. Bueno ◽  
Daniele Y. Sunaga ◽  
Gerson S. Kobayashi ◽  
Meire Aguena ◽  
Cassio E.R. Amaral ◽  
...  
Keyword(s):  
Stem Cells ◽  
Signaling Pathways ◽  
Candidate Genes ◽  
Cleft Lip ◽  
Cleft Lip And Palate

scholarly journals Deciduous Dental Pulp Stem Cells for Maxillary Alveolar Reconstruction in Cleft Lip and Palate Patients

2020 ◽  
Vol 2020 ◽  
pp. 1-9 ◽  
Author(s):  
Daniela Y. S. Tanikawa ◽  
Carla C. G. Pinheiro ◽  
Maria Cristina A. Almeida ◽  
Claudia R. G. C. M. Oliveira ◽  
Renata de Almeida Coudry ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Cleft Lip ◽  
Cleft Lip And Palate ◽  
Alveolar Bone ◽  
Dental Pulp Stem Cells ◽  
Iliac Crest Bone Graft ◽  
Dental Eruption ◽  
Donor Site Pain ◽  
Historical Controls

Background. To reduce morbidity to cleft patients, new approaches have been developed and here, we report for the first time the use of deciduous dental pulp stem cells (DDPSC) associated with a hydroxyapatite-collagen sponge (Bio-Oss Collagen® 250 mg, Geistlich) for closing alveolar defects during secondary dental eruption, further comparing these results to historical controls. Methods. Six patients, aged 8 to 12, were selected. Autologous DDPSC were isolated from each patient, then associated with the biomaterial and this bone tissue engineered set was used to fill the alveolar defect. Computed tomography was performed to assess both preoperative and 6- and 12-month postoperative outcomes. Overall morbidity was recorded. Historical controls consisted of sixteen patients previously selected and randomly assigned to group one (rhBMP-2) or group two (iliac crest bone graft). Results. DDPSC could be isolated and characterized as mesenchymal stem cells. Progressive alveolar bone union has occurred in all patients. Similarly to group two 75.4%, SD±4.0, p>0.999, but statistically different from group one (59.6%, SD±9.9, p=0.001), completion of the defect with 75.6% (SD±4.8) of bone filling was detected 6 months postoperatively. Dental eruption routinely occurred in 66.7% of patients. No complications were detected, in comparison to significant swelling in 37.5% of group one patients and significant donor site pain in 87.5% of group two. Conclusion. For this selected group of patients, DDPSC therapy resulted in satisfactory bone healing with excellent feasibility and safety, which adds significantly to the prospect of stem cell use in clinical settings. Clinical Question/Level of Evidence. Therapeutic, II. This trial is registered with https://clinicaltrials.gov/ct2/show/NCT01932164?term=NCT01932164&rank=1.

scholarly journals Medical Sequencing of Candidate Genes for Nonsyndromic Cleft Lip and Palate

PLoS Genetics ◽  
2005 ◽  
Vol 1 (6) ◽  
pp. e64 ◽  
Author(s):  
Alexandre R Vieira ◽  
Joseph R Avila ◽  
Sandra Daack-Hirsch ◽  
Ecaterina Dragan ◽  
Têmis M Félix ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Cleft Lip ◽  
Cleft Lip And Palate

Analysis of the candidate genes responsible for non-syndromic cleft lip and palate in Japanese people

2000 ◽  
Vol 99 (2) ◽  
pp. 105 ◽  
Author(s):  
Atsuko TANABE ◽  
Shigeru TAKETANI ◽  
Yoko ENDO-ICHIKAWA ◽  
Rikio TOKUNAGA ◽  
Yutaka OGAWA ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Cleft Lip ◽  
Cleft Lip And Palate ◽  
Japanese People

Association studies of candidate genes and cleft lip and palate taking into consideration geographical origin

2011 ◽  
Vol 119 (6) ◽  
pp. 413-417 ◽  
Author(s):  
Baiba Lace ◽  
Inga Kempa ◽  
Linda Piekuse ◽  
Ieva Grinfelde ◽  
Janis Klovins ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Cleft Lip ◽  
Cleft Lip And Palate ◽  
Geographical Origin ◽  
Association Studies

Studies of the Candidate Genes TGFB2, MSX1, TGFA, and TGFB3 in the Etiology of Cleft Lip and Palate in the Philippines

1997 ◽  
Vol 34 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Andrew C. Lidral ◽  
Jeffrey C. Murray ◽  
Kenneth H. Buetow ◽  
Ann M. Basart ◽  
Heidi Schearer ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Cleft Lip ◽  
Cleft Lip And Palate ◽  
The Philippines

scholarly journals Repair of alveolar cleft bone defects by bone collagen particles combined with human umbilical cord mesenchymal stem cells in rabbit

2020 ◽  
Author(s):  
Xue-Cheng Sun ◽  
Hu Wang ◽  
Jian-Hui Li ◽  
Dan Zhang ◽  
Xu Ma ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Bone Repair ◽  
Cleft Lip ◽  
Cleft Lip And Palate ◽  
Bone Defects ◽  
Bone Collagen ◽  
Human Umbilical Cord ◽  
Alveolar Cleft

Abstract Background: Alveolar cleft is a kind of cleft lip and palate, which seriously affects the physical and mental health of patients. In this study ， the model of the alveolar cleft phenotype was established in rabbits to evaluate the effect of bone collagen particles combined with human umbilical cord mesenchymal stem cells (HUC-MSCs) on the repair of alveolar cleft bone defects. Methods : The model of alveolar clefts in rabbits was established by removing the incisors on the left side of the upper jaw. Bone collagen particles combined with hUC-MSCs were implanted in the defect area. Blood biochemical analysis was performed after 3 months. Skull tissues were harvested for gross observation, and micro-focus computerized tomography (micro-CT) analysis. Tissues were harvested for histological and immunohistochemical staining. The experiments were repeated 6 months after surgery. Results: The bone collagen particles and HUC-MSCs have good biological safety. In addition, both can promote the regeneration of incisor. Bone collagen particles combined with hUC-MSCs were much better than those used alone in inducing bone repair and regeneration. Conclusions: The method of HUC-MSCs combined with bone collagen particle material to fill a bone defect site is simple, rapid and suitable for the treatment of alveolar cleft bone defects.

scholarly journals Bone Regeneration in Artificial Jaw Cleft by Use of Carbonated Hydroxyapatite Particles and Mesenchymal Stem Cells Derived from Iliac Bone

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Motoko Yoshioka ◽  
Kotaro Tanimoto ◽  
Yuki Tanne ◽  
Keisuke Sumi ◽  
Tetsuya Awada ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Bone Defect ◽  
Cleft Lip ◽  
Cleft Lip And Palate ◽  
Iliac Bone ◽  
Invasive Technique ◽  
Carbonated Hydroxyapatite ◽  
X Ray ◽  
Experimental Side

Objectives of the Study. Cleft lip and palate (CLP) is a prevalent congenital anomaly in the orofacial region. Autogenous iliac bone grafting has been frequently employed for the closure of bone defects at the jaw cleft site. Since the related surgical procedures are quite invasive for patients, it is of great importance to develop a new less invasive technique. The aim of this study was to examine bone regeneration with mesenchyme stem cells (MSCs) for the treatment of bone defect in artificially created jaw cleft in dogs.Materials and Methods. A bone defect was prepared bilaterally in the upper incisor regions of beagle dogs. MSCs derived from iliac bone marrow were cultured and transplanted with carbonated hydroxyapatite (CAP) particles into the bone defect area. The bone regeneration was evaluated by standardized occlusal X-ray examination and histological observation.Results. Six months after the transplantation, perfect closure of the jaw cleft was achieved on the experimental side. The X-ray and histological examination revealed that the regenerated bone on the experimental side was almost equivalent to the original bone adjoining the jaw cleft.Conclusion. It was suggested that the application of MSCs with CAP particles can become a new treatment modality for bone regeneration for CLP patients.

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