scholarly journals 03-P121 Expression pattern of proximodistal markers during avian lung development

2009 ◽  
Vol 126 ◽  
pp. S102
Author(s):  
Takashi Miura
Keyword(s):  
Expression Pattern ◽  
Lung Development ◽  
Avian Lung

Dexamethasone Effect on Embryonic Chick Respiratory Epithelium

1982 ◽  
Vol 40 ◽  
pp. 248-249
Author(s):  
M.R. Richter ◽  
R.V. Blystone
Keyword(s):  
Lung Development ◽  
Critical Role ◽  
Respiratory Epithelium ◽  
Chick Embryos ◽  
Embryonic Chick ◽  
White Leghorn ◽  
Lung Maturation ◽  
Synthetic Analogs ◽  
Lung Physiology ◽  
Avian Lung

Dexamethasone and other synthetic analogs of corticosteroids have been employed clinically as enhancers of lung development. The mechanism(s) by which this steroid induction of later lung maturation operates is not clear. This study reports the effect on lung epithelia of dexamethasone administered at different intervals during development. White Leghorn chick embryos were used so as to remove possible maternal and placental influences on the exogenously applied steroid. Avian lung architecture does vary from mammals; however, respiratory surfactant produced by the lung epithelia serves an equally critical role in avian lung physiology.

scholarly journals MicroRNA-127 modulates fetal lung development

2009 ◽  
Vol 37 (3) ◽  
pp. 268-278 ◽  
Author(s):  
Manoj Bhaskaran ◽  
Yang Wang ◽  
Honghao Zhang ◽  
Tingting Weng ◽  
Pradyumna Baviskar ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Lung Development ◽  
Fetal Lung ◽  
Mirna Microarray ◽  
Mirna Expression Pattern ◽  
Opposite Trend ◽  
Fetal Lung Development ◽  
Terminal Bud ◽  
Lung Organ Culture

MicroRNAs (miRNAs) are small endogenous RNAs and are widely regarded as one of the most important regulators of gene expression in both plants and animals. To define the roles of miRNAs in fetal lung development, we profiled the miRNA expression pattern during lung development with a miRNA microarray. We identified 21 miRNAs that showed significant changes in expression during lung development. These miRNAs were grouped into four distinct clusters based on their expression pattern. Cluster 1 contained miRNAs whose expression increased as development progressed, while clusters 2 and 3 showed the opposite trend of expression. miRNAs in cluster 4 including miRNA-127 (miR-127) had the highest expression at the late stage of fetal lung development. Quantitative real-time PCR validated the microarray results of six selected miRNAs. In situ hybridization demonstrated that miR-127 expression gradually shifted from mesenchymal cells to epithelial cells as development progressed. Overexpression of miR-127 in fetal lung organ culture significantly decreased the terminal bud count, increased terminal and internal bud sizes, and caused unevenness in bud sizes, indicating improper development. These findings suggest that miR-127 may have an important role in fetal lung development.

scholarly journals Lung elastic fibres from embryonic to adult birds

2008 ◽  
Vol 14 (S3) ◽  
pp. 113-114
Author(s):  
Ana Honório ◽  
Maria de Lurdes Pinto ◽  
Carlos Gonçalves ◽  
Vasco Bairos
Keyword(s):  
Image Analysis ◽  
Experimental Model ◽  
Analytical Methods ◽  
Lung Development ◽  
Domestic Fowl ◽  
Gallus Gallus ◽  
Elastic Fibres ◽  
Morphological Studies ◽  
Avian Lung

In mammals it is well known the role of the elastic fibres in the lung development and its fundamental importance in the process of distending and recoil of the organ as a whole. The bird's lungs are compact and virtually non-expansible.After morphological studies on elastic fibres distribution and quantification by image analysis we carried out a study on biochemical methods to quantify elastin in the avian lung, using domestic fowl (Gallus gallus) as an experimental model. We made use of two analytical methods for the elastin determination in these animal lungs from the 14th day of hatching until the 42nd day after hatchling.

scholarly journals The Role of Ephrins-B1 and -B2 During Fetal Rat Lung Development

2015 ◽  
Vol 35 (1) ◽  
pp. 104-115 ◽  
Author(s):  
Francisca O. Peixoto ◽  
Patrícia Pereira-Terra ◽  
Rute S. Moura ◽  
Emanuel Carvalho-Dias ◽  
Jorge Correia-Pinto ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Lung Development ◽  
Fetal Lung ◽  
Cardiovascular Development ◽  
Rat Lung ◽  
Growth Promoters ◽  
Lung Morphogenesis ◽  
Rat Lungs ◽  
Fetal Rat

Background/ Aims: The knowledge of the molecular network that governs fetal lung branching is an essential step towards the discovery of novel therapeutic targets against pulmonary pathologies. Lung consists of two highly branched systems: airways and vasculature. Ephrins and its receptors, Eph, have been implicated in cardiovascular development, angiogenesis and vascular remodeling. This study aims to clarify the role of these factors during lung morphogenesis. Methods: Ephrins-B1, -B2 and receptor EphB4 expression pattern was assessed in fetal rat lungs between 15.5 and 21.5 days post-conception, by immunohistochemistry. Fetal rat lungs were harvested at 13.5 dpc, cultured during 4 days and treated with increasing doses of ephrins-B1 and -B2 and the activity of key signaling pathways was assessed. Results: Ephrin-B1 presents mesenchymal expression, whereas ephrin-B2 and its receptor EphB4 were expressed by the epithelium. Both ephrins stimulated pulmonary branching. Moreover, while ephrin-B1 did not affect the pathways studied, ephrin-B2 supplementation decreased activity of JNK, ERK and STAT. This study characterizes the expression pattern of ephrins-B1, -B2 and EphB4 receptor throughout rat lung development. Conclusion: Our data highlight a possible role of ephrins as molecular stimulators of lung morphogenesis. Moreover, it supports the idea that classical vascular factors might play a role as airway growth promoters.

Connective tissue growth factor expression pattern in lung development

2010 ◽  
Vol 36 (8) ◽  
pp. 441-450 ◽  
Author(s):  
Carmen Mesas Burgos ◽  
Magnus Nord ◽  
Abraham Roos ◽  
Lukas Didon ◽  
Ann-Christine Eklöf ◽  
...  
Keyword(s):  
Growth Factor ◽  
Connective Tissue ◽  
Expression Pattern ◽  
Connective Tissue Growth Factor ◽  
Lung Development ◽  
Tissue Growth ◽  
Factor Expression ◽  
Growth Factor Expression

Effects of hypoxia or hyperoxia on the lung of the chick embryo

1989 ◽  
Vol 67 (5) ◽  
pp. 515-519 ◽  
Author(s):  
Lijing Xu ◽  
Jacopo P. Mortola
Keyword(s):  
Chick Embryo ◽  
Lung Development ◽  
Chronic Hypoxia ◽  
Ventilatory Response ◽  
Mechanical Stretch ◽  
Mechanical Effect ◽  
Cellular Component ◽  
Chick Embryos ◽  
Breathing Cycle ◽  
Avian Lung

Newborn mammals in chronic hypoxia or hyperoxia experience, respectively, an increase or decrease in lung weight:body weight ratios, possibly because of the mechanical effect on the lung accompanying the ventilatory response. Because the avian lung does not expand or contract with the breathing cycle, we asked whether or not qualitatively similar changes could be observed in the lung of chick embryos incubated in hypoxic or hyperoxic conditions. Hypoxic embryos (10% O2, days 14–18) were smaller than controls incubated in normoxia, with higher hematocrit values and larger lung weight:body weight ratios (both wet and dry). Both the total pulmonary DNA (reflecting the cellular component) and the DNA concentration were decreased in hypoxia. Hyperoxic embryos (50% O2, days 7–18 or days 14–18) had lower hematocrit values and smaller dry lung weight:body weight ratios than controls, with similar DNA concentrations. In general, the differences from controls were more apparent in those embryos hyperoxic from day 14 to 18 of incubation than from day 7 to 18. We conclude that changes in lung weights qualitatively similar to those occurring in the chronically hypoxic or hyperoxic newborn mammal can also be observed in the hypoxic or hyperoxic chick embryo, suggesting that they are not necessarily caused by changes in mechanical stretch on the lung.Key words: lung development, lung DNA, avian growth.

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