Grinding and polishing instead of sectioning for the tissue samples with a graft: Implications for light and electron microscopy

Micron ◽  
2016 ◽  
Vol 85 ◽  
pp. 1-7 ◽  
Author(s):  
Rinat A. Mukhamadiyarov ◽  
Victoria V. Sevostyanova ◽  
Daria K. Shishkova ◽  
Andrey V. Nokhrin ◽  
Olga D. Sidorova ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Tissue Samples ◽  
Grinding And Polishing

Condensed tannins in sainfoin. II. Occurrence and changes during leaf development

1995 ◽  
Vol 73 (10) ◽  
pp. 1540-1547 ◽  
Author(s):  
G. L. Lees ◽  
M. Y. Gruber ◽  
N. H. Suttill
Keyword(s):  
Electron Microscopy ◽  
Leaf Development ◽  
Condensed Tannins ◽  
Histological Study ◽  
Condensed Tannin ◽  
Light And Electron Microscopy ◽  
Leaf Primordia ◽  
Tissue Samples ◽  
Onobrychis Viciifolia ◽  
Mature Phase

A histological study examined condensed tannin (CT) formation in plant tissue samples taken from the meristematic area of very young sainfoin (Onobrychis viciifolia Scop.) seedlings and from leaflets sampled at various stages of development in mature plants growing in the greenhouse. Light and electron microscopy revealed no CT in the seedling meristem and leaf primordia, but CTs were seen very early in leaf development forming first in the vacuoles of discrete cells of the abaxial subepidermal layer when the leaflets were recognizable, but still folded. Immature leaflets collected from the growing point of a mature sainfoin plant show similar CT formation with the abaxial cell vacuoles filled with CT when the new leaves have reached the 90°-fold stage. As the leaflets unfold and mature, CTs begin to appear in the vacuoles of small, but discrete cells in the adaxial subepidermal layer while the tannin-containing cells in the abaxial subepidermal layer begin to lose CT. The CT continues to increase in the adaxial layer until typical enlarged tannin idioblasts or sacs are observed at full leaflet expansion and maturity. By this stage, the vacuoles in the abaxial layer are almost empty. In senescing leaflet samples collected from the leaf rachis attached to the last and second to last node near the base of the plant, the cells in both subepidermal layers have lost the majority of the CT that was originally formed. At senescence all tannin-containing cells appear as empty shells. We speculate that a finite amount of CT is formed in the two subepidermal layers of new leaves at different stages of early leaf development, does not increase during the mature phase, and is catabolized in older leaves and during senescence. Key words: condensed tannins, sainfoin, Onobrychis viciifolia, leaf development.

scholarly journals In situ strategy for biomedical target localization via nanogold nucleation and secondary growth

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Akira Sawaguchi ◽  
Takeshi Kamimura ◽  
Nobuyasu Takahashi ◽  
Atsushi Yamashita ◽  
Yujiro Asada ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Self Assembly ◽  
Light And Electron Microscopy ◽  
Secondary Growth ◽  
Spatial Location ◽  
Tissue Samples ◽  
Cell Tissue ◽  
Nanogold Particles ◽  
And Function

AbstractImmunocytochemistry visualizes the exact spatial location of target molecules. The most common strategy for ultrastructural immunocytochemistry is the conjugation of nanogold particles to antibodies as probes. However, conventional nanogold labelling requires time-consuming nanogold probe preparation and ultrathin sectioning of cell/tissue samples. Here, we introduce an in situ strategy involving nanogold nucleation in immunoenzymatic products on universal paraffin/cryostat sections and provide unique insight into nanogold development under hot-humid air conditions. Nanogold particles were specifically localized on kidney podocytes to target synaptopodin. Transmission electron microscopy revealed secondary growth and self-assembly that could be experimentally controlled by bovine serum albumin stabilization and phosphate-buffered saline acceleration. Valuable retrospective nanogold labelling for gastric H+/K+-ATPase was achieved on vintage immunoenzymatic deposits after a long lapse of 15 years (i.e., 15-year-old deposits). The present in situ nanogold labelling is anticipated to fill the gap between light and electron microscopy to correlate cell/tissue structure and function.

scholarly journals Preservation of Fluorescence Signal and Imaging Optimization for Integrated Light and Electron Microscopy

2021 ◽  
Vol 9 ◽  
Author(s):  
Pieter Baatsen ◽  
Sergio Gabarre ◽  
Katlijn Vints ◽  
Rosanne Wouters ◽  
Dorien Vandael ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Fluorescent Proteins ◽  
Fluorescent Microscopy ◽  
Light And Electron Microscopy ◽  
High Resolution Electron Microscopy ◽  
Fluorescence Signal ◽  
Microscopy Imaging ◽  
Tissue Samples ◽  
Rapid Acquisition

Life science research often needs to define where molecules are located within the complex environment of a cell or tissue. Genetically encoded fluorescent proteins and or fluorescence affinity-labeling are the go-to methods. Although recent fluorescent microscopy methods can provide localization of fluorescent molecules with relatively high resolution, an ultrastructural context is missing. This is solved by imaging a region of interest with correlative light and electron microscopy (CLEM). We have adopted a protocol that preserves both genetically-encoded and antibody-derived fluorescent signals in resin-embedded cell and tissue samples and provides high-resolution electron microscopy imaging of the same thin section. This method is particularly suitable for dedicated CLEM instruments that combine fluorescence and electron microscopy optics. In addition, we optimized scanning EM imaging parameters for samples of varying thicknesses. These protocols will enable rapid acquisition of CLEM information from samples and can be adapted for three-dimensional EM.

Initiation of placentation in the pig

1984 ◽  
Vol 42 ◽  
pp. 730-731
Author(s):  
J.L. Keys
Keyword(s):  
Electron Microscopy ◽  
Developmental Stages ◽  
Morphological Changes ◽  
Light And Electron Microscopy ◽  
Microscopic Structure ◽  
Initial Attachment ◽  
Tissue Samples ◽  
The Past ◽  
Early Developmental Stages ◽  
Early Developmental

The microscopic structure of the mature placenta in domesticated species has been described in many publications but the early developmental stages have only been studied in detail within the past decade. The earliest date previously cited for initial attachment of the porcine trophoblast to the maternal epithelium is 14 to 18 days of gestation. This investigation was undertaken to establish morphological changes in the maternal epithelium in preparation for this event.Four or 5 nulliparous gilts were slaughtered on each of d10 and d13 of the estrous cycle and pregnancy (d0=1st d of estrus), and tissue samples prepared for correlative light and electron microscopy. Samples were compared between gilts on equivalent days of the cycle/pregnancy in order to determine endometrial changes specific to pregnancy. Sites adjacent to embryos were contrasted with those lacking trophoblastic contact within the same animal to investigate whether epithelial adaptations for attachment are localized to trophoblast proximity or represent a generalized uterine response.

scholarly journals Alport’s syndrome and benign familial haematuria: Light and electron microscopic studies of the kidney

2008 ◽  
Vol 136 (Suppl. 4) ◽  
pp. 275-281
Author(s):  
Jovan Dimitrijevic ◽  
Vera Todorovic ◽  
Anastasija Aleksic ◽  
Dijana Jovanovic ◽  
Dijana Pilcevic ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Light And Electron Microscopy ◽  
Definitive Diagnosis ◽  
Basement Membranes ◽  
Tissue Slices ◽  
Electron Microscopic ◽  
Tissue Samples ◽  
Hereditary Nephritis ◽  
Hereditary Nephropathy

INTRODUCTION. Hereditary nephropathy is clinically characterized by the familial occurrence in successive generations of progressive haematuric nephritis and neural hearing loss. Hereditary nephropathy of Alport?s syndrome (AS) and benign familial (recurrent) haematuria (BFH) are morphologically characterized by specific and diagnostically important thickening and splitting of lamina densa of the glomerular basement membranes. Those lesions can be recognized only by electron microscopy. Hereditary nephritis is usually present clinically with haematuria, and new mutations without a family history of haematuria. It is therefore important to differentiate hereditary nephritis from BFH and no familial haematuria. Thus, electron microscopy is essential in diagnosis of haematuria. OBJECTIVE. The aim of this study was to describe, by light microscopy, constellation of renal alterations by which hereditary nephropathy can be recognized with high probability as well as to compare the diagnostic validity of the findings observed by light and electron microscopy in AS and BFH. METHOD. We examined 48 renal biopsies of the patients with hereditary nephoropathies by light and electron microscopy. Tissue samples were fixed in buffered paraformaldehyde and embedded in paraffin for long-term preservation. For the electron microscopy analysis, the following fixation in 4% glutaraldehyde tissue was postfixed in 1% osmium tetroxide. Thereafter, the following dehydration procedure tissue slices were embedded in epon. RESULTS. Our results demonstrated that the interstitial foam cells, foetal-like glomeruli, minimal glomerular abnormalities with stain less intense in basement membranes, mild irregular mesangial widening, focal thickening of Bowman?s capsule, foci of dilatation tubules, tubular ectasia and atrophy, erythrocyte tubules casts were present in hereditary nephritis. Additionally, light microscopic biopsy findings in patients with BFH were either normal or revealed minor changes (e.g. increased mesangial matrix). All biopsies were reevaluated by electron microscopy and ultrastructural findings confirmed the diagnosis of hereditary nephropathies. CONCLUSION. The findings observed by light microscopy represent an important step that leads to a definitive diagnosis of AS and BFH. The definitive diagnosis, however, depends on electron microscopy.

The Morphology of Vacuolated Cells in the Liver Following Administration of Vitamin A.

1973 ◽  
Vol 31 ◽  
pp. 408-409
Author(s):  
Odell T. Minick ◽  
Hidejiro Yokoo ◽  
Fawzia Batti
Keyword(s):  
Electron Microscopy ◽  
Body Weight ◽  
Vitamin A ◽  
Light And Electron Microscopy ◽  
Liver Cells ◽  
Prominent Feature ◽  
Vascular Space ◽  
Vacuolated Cell ◽  
Vacuolated Cells ◽  
Vacuolated Cytoplasm

Vacuolated cells in the liver of young rats were studied by light and electron microscopy following the administration of vitamin A (200 units per gram of body weight). Their characteristics were compared with similar cells found in untreated animals.In rats given vitamin A, cells with vacuolated cytoplasm were a prominent feature. These cells were found mostly in a perisinusoidal location, although some appeared to be in between liver cells (Fig. 1). Electron microscopy confirmed their location in Disse's space adjacent to the sinusoid and in recesses between liver cells. Some appeared to be bordering the lumen of the sinusoid, but careful observation usually revealed a tenuous endothelial process separating the vacuolated cell from the vascular space. In appropriate sections, fenestrations in the thin endothelial processes were noted (Fig. 2, arrow).

Control of Autogenous Vein Grafts Prior to Reimplantation, By Electron Microscopy

1972 ◽  
Vol 30 ◽  
pp. 106-107
Author(s):  
John H. L. Watson ◽  
John L. Swedo ◽  
M. Vrandecic
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Physiological Saline ◽  
Experimental Conditions ◽  
Vein Grafts ◽  
Arterial Blood ◽  
Saphenous Veins ◽  
Autogenous Vein ◽  
Control Of Parameters ◽  
Post Implantation

The ambient temperature and the nature of the storage fluids may well have significant effects upon the post-implantation behavior of venus autografts. A first step in the investigation of such effects is reported here. Experimental conditions have been set which approximate actual operating room procedures. Saphenous veins from dogs have been used as models in the experiments. After removal from the dogs the veins were kept for two hours under four different experimental conditions, viz at either 4°C or 23°C in either physiological saline or whole canine arterial blood. At the end of the two hours they were prepared for light and electron microscopy. Since no obvious changes or damage could be seen in the veins by light microscopy, even with the advantage of tissue specific stains, it was essential that the control of parameters for successful grafts be set by electron microscopy.

Ultrastructure of two neoplasms in culture compared with original biopsies

1984 ◽  
Vol 42 ◽  
pp. 50-51
Author(s):  
Joseph M. Harb ◽  
James T. Casper ◽  
Vlcki Piaskowski
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Biopsy Specimen ◽  
Cultured Cells ◽  
Light And Electron Microscopy ◽  
Human Tumor ◽  
Soft Agar ◽  
Human Tumor Cells ◽  
Morphological Distinction ◽  
Tumor Types

The application of tissue culture and the newer methodologies of direct cloning and colony formation of human tumor cells in soft agar hold promise as valuable modalities for a variety of diagnostic studies, which include morphological distinction between tumor types by electron microscopy (EM). We present here two cases in which cells in culture expressed distinct morphological features not apparent in the original biopsy specimen. Evaluation of the original biopsies by light and electron microscopy indicated both neoplasms to be undifferentiated sarcomas. Colonies of cells propagated in soft agar displayed features of rhabdomyoblasts in one case, and cultured cells of the second biopsy expressed features of Ewing's sarcoma.

The Effect of Vitamin A on the Intermittent Nitrogen Dioxide Gas Exposure in Hamsters

1976 ◽  
Vol 34 ◽  
pp. 176-177
Author(s):  
J.C.S. Kim ◽  
M.G. Jourden ◽  
E.S. Carlisle
Keyword(s):  
Electron Microscopy ◽  
Vitamin A ◽  
Nitrogen Dioxide ◽  
Chronic Exposure ◽  
Light And Electron Microscopy ◽  
Specific Effect ◽  
Deficient Diet ◽  
Intermittent Exposure ◽  
Hypovitaminosis A ◽  
Gas Exposure

Chronic exposure to nitrogen dioxide in rodents has shown that injury reaches a maximum after 24 hours, and a reparative adaptive phase follows (1). Damage occurring in the terminal bronchioles and proximal portions of the alveolar ducts in rats has been extensively studied by both light and electron microscopy (1).The present study was undertaken to compare the response of lung tissue to intermittent exposure to 10 ppm of nitrogen dioxide gas for 4 hours per week, while the hamsters were on a vitamin A deficient diet. Ultrastructural observations made from lung tissues obtained from non-gas exposed, hypovitaminosis A animals and gas exposed animals fed a regular commercially prepared diet have been compared to elucidate the specific effect of vitamin A on nitrogen dioxide gas exposure. The interaction occurring between vitamin A and nitrogen dioxide gas has not previously been investigated.

mRNA localization by Electron microscopic in situ hybridization

1991 ◽  
Vol 49 ◽  
pp. 430-431
Author(s):  
J. A. Pollock ◽  
M. Martone ◽  
T. Deerinck ◽  
M. H. Ellisman
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Nucleic Acids ◽  
Recombinant Dna ◽  
Light And Electron Microscopy ◽  
Electron Microscopic ◽  
High Voltage Electron Microscopy ◽  
Functional Sites ◽  
Voltage Electron

Localization of specific proteins in cells by both light and electron microscopy has been facilitate by the availability of antibodies that recognize unique features of these proteins. High resolution localization studies conducted over the last 25 years have allowed biologists to study the synthesis, translocation and ultimate functional sites for many important classes of proteins. Recently, recombinant DNA techniques in molecular biology have allowed the production of specific probes for localization of nucleic acids by “in situ” hybridization. The availability of these probes potentially opens a new set of questions to experimental investigation regarding the subcellular distribution of specific DNA's and RNA's. Nucleic acids have a much lower “copy number” per cell than a typical protein, ranging from one copy to perhaps several thousand. Therefore, sensitive, high resolution techniques are required. There are several reasons why Intermediate Voltage Electron Microscopy (IVEM) and High Voltage Electron Microscopy (HVEM) are most useful for localization of nucleic acids in situ.

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