A new plan for determining drug pharmacokinetics by establishment of urinary excretion pattern. Spectrofluorimetric application on Lomeflox® tablets

2019 ◽  
Vol 148 ◽  
pp. 419-423
Author(s):  
Shereen A. Boltia ◽  
Aya T. Soudi ◽  
Eman S. Elzanfaly ◽  
Hala E. Zaazaa
Keyword(s):  
Urinary Excretion ◽  
Excretion Pattern ◽  
Drug Pharmacokinetics

scholarly journals Solid-Phase Extraction and Reverse-Phase HPLC: Application to Study the Urinary Excretion Pattern of Benzophenone-3 and its Metabolite 2,4-Dihydroxybenzophenone in Human Urine

2008 ◽  
Vol 3 ◽  
pp. ACI.S396 ◽  
Author(s):  
Helena Gonzalez ◽  
Carl-Eric Jacobson ◽  
Ann-Marie Wennberg ◽  
Olle Larkö ◽  
Anne Farbrot
Keyword(s):  
Solid Phase Extraction ◽  
Urinary Excretion ◽  
Human Urine ◽  
Solid Phase ◽  
Whole Body ◽  
Phase Extraction ◽  
Reverse Phase Hplc ◽  
Reverse Phase ◽  
Excretion Pattern ◽  
Relative Standard

Background Benzophenone-3 (BZ-3) is a common ultraviolet (UV) absorbing compound in sunscreens. It is the most bioavailable species of all UV-absorbing compounds after topical application and can be found in plasma and urine. Objectives The aim of this study was to develop a reverse-phase high performance liquid chromatography (HPLC) method for determining the amounts BZ-3 and its metabolite 2,4-dihydroxybenzophenone (DHB) in human urine. The method had to be suitable for handling a large number of samples. It also had to be rapid and simple, but still sensitive, accurate and reproducible. The assay was applied to study the urinary excretion pattern after repeated whole-body applications of a commercial sunscreen, containing 4% BZ-3, to 25 healthy volunteers. Methods Each sample was analyzed with regard to both conjugated/non-conjugated BZ-3 and conjugated/non-conjugated DHB, since both BZ-3 and DHB are extensively conjugated in the body. Solid-phase extraction (SPE) with C8 columns was followed by reverse-phase HPLC. For separation a Genesis C18 column was used with an acethonitrile-water mobile phase and the UV-detector was set at 287 nm. Results The assay was linear r 2 > 0.99, with detection limits for BZ-3 and DHB of 0.01 µmol L-1 and 0.16 µmol L-1 respectively. Relative standard deviation (RSD) was less than 10% for BZ-3 and less than 13% for DHB. The excretion pattern varied among the human volunteers; we discerned different patterns among the individuals. Conclusions The reverse-phase HPLC assay and extraction procedures developed are suitable for use when a large number of samples need to be analyzed and the method fulfilled our objectives. The differences in excretion pattern may be due to differences in enzyme activity but further studies, especially about genetic polymorphism, need to be performed to verify this finding.

DISTURBED URINARY EXCRETION PATTERN OF OESTROGENS IN NEWBORNS WITH CONGENITAL DISLOCATION OF THE HIP

1961 ◽  
Vol 37 (3) ◽  
pp. 423-426 ◽  
Author(s):  
L. Andrén ◽  
N. E. Borglin
Keyword(s):  
Urinary Excretion ◽  
Congenital Dislocation ◽  
Excretion Pattern ◽  
Dislocation Of The Hip

ABSTRACT There is an increased excretion of oestradiol-17β and oestrone in newborns with congenital dislocation of the hip. This increase is ascribed to an inborn and probably hereditary error of metabolism.

THE URINARY EXCRETION PATTERN OF TRACE METALS IN DIABETES MELLITUS

1962 ◽  
Vol 244 (3) ◽  
pp. 282-289 ◽  
Author(s):  
Lawrence E. Meltzer ◽  
Julia Rutman ◽  
Philip George ◽  
Robert Rutman ◽  
J Roderick Kitchell
Keyword(s):  
Diabetes Mellitus ◽  
Trace Metals ◽  
Urinary Excretion ◽  
Excretion Pattern

scholarly journals Urinary excretion pattern of triethyllead,diethyllead and inorganic lead in the tetraethyllead poisoning.

1981 ◽  
Vol 19 (2) ◽  
pp. 125-131 ◽  
Author(s):  
Yukio YAMAMURA ◽  
Fumio ARAI ◽  
Hiroshi YAMAUCHI
Keyword(s):  
Urinary Excretion ◽  
Excretion Pattern ◽  
Inorganic Lead

Urinary Excretion Pattern of Main Glycosaminoglycans in Stone Formers and Controls

1985 ◽  
pp. 355-358 ◽  
Author(s):  
A. Martelli ◽  
B. Marchesini ◽  
P. Buli ◽  
F. Lambertini ◽  
R. Rusconi
Keyword(s):  
Urinary Excretion ◽  
Excretion Pattern ◽  
Stone Formers
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