scholarly journals Urinary Excretion Pattern of Serotonin and 5-Hydroxyindole Acetic Acid in Ultraviolet Induced Erythema**From the Institute of Radiopathology, University of Groningen, Groningen, The Netherlands.

1968 ◽  
Vol 50 (1) ◽  
pp. 1-8 ◽  
Author(s):  
T.S. Veninga ◽  
J.E. de Boer
Keyword(s):  
Acetic Acid ◽  
The Netherlands ◽  
Urinary Excretion ◽  
Excretion Pattern

scholarly journals Solid-Phase Extraction and Reverse-Phase HPLC: Application to Study the Urinary Excretion Pattern of Benzophenone-3 and its Metabolite 2,4-Dihydroxybenzophenone in Human Urine

2008 ◽  
Vol 3 ◽  
pp. ACI.S396 ◽  
Author(s):  
Helena Gonzalez ◽  
Carl-Eric Jacobson ◽  
Ann-Marie Wennberg ◽  
Olle Larkö ◽  
Anne Farbrot
Keyword(s):  
Solid Phase Extraction ◽  
Urinary Excretion ◽  
Human Urine ◽  
Solid Phase ◽  
Whole Body ◽  
Phase Extraction ◽  
Reverse Phase Hplc ◽  
Reverse Phase ◽  
Excretion Pattern ◽  
Relative Standard

Background Benzophenone-3 (BZ-3) is a common ultraviolet (UV) absorbing compound in sunscreens. It is the most bioavailable species of all UV-absorbing compounds after topical application and can be found in plasma and urine. Objectives The aim of this study was to develop a reverse-phase high performance liquid chromatography (HPLC) method for determining the amounts BZ-3 and its metabolite 2,4-dihydroxybenzophenone (DHB) in human urine. The method had to be suitable for handling a large number of samples. It also had to be rapid and simple, but still sensitive, accurate and reproducible. The assay was applied to study the urinary excretion pattern after repeated whole-body applications of a commercial sunscreen, containing 4% BZ-3, to 25 healthy volunteers. Methods Each sample was analyzed with regard to both conjugated/non-conjugated BZ-3 and conjugated/non-conjugated DHB, since both BZ-3 and DHB are extensively conjugated in the body. Solid-phase extraction (SPE) with C8 columns was followed by reverse-phase HPLC. For separation a Genesis C18 column was used with an acethonitrile-water mobile phase and the UV-detector was set at 287 nm. Results The assay was linear r 2 > 0.99, with detection limits for BZ-3 and DHB of 0.01 µmol L-1 and 0.16 µmol L-1 respectively. Relative standard deviation (RSD) was less than 10% for BZ-3 and less than 13% for DHB. The excretion pattern varied among the human volunteers; we discerned different patterns among the individuals. Conclusions The reverse-phase HPLC assay and extraction procedures developed are suitable for use when a large number of samples need to be analyzed and the method fulfilled our objectives. The differences in excretion pattern may be due to differences in enzyme activity but further studies, especially about genetic polymorphism, need to be performed to verify this finding.

scholarly journals Laboratory testing for recent alcohol consumption: comparison of ethanol, methanol, and 5-hydroxytryptophol

1996 ◽  
Vol 42 (4) ◽  
pp. 618-624 ◽  
Author(s):  
A Helander ◽  
O Beck ◽  
A W Jones
Keyword(s):  
Acetic Acid ◽  
Alcohol Consumption ◽  
Gender Difference ◽  
Healthy Volunteers ◽  
Laboratory Testing ◽  
Background Concentrations ◽  
Excretion Pattern ◽  
Sensitive Method ◽  
Alcohol Dependent

Abstract The ratio of 5-hydroxytryptophol to 5-hydroxyindole-3-acetic acid (5HTOL/5HIAA) in urine was compared with concentrations of ethanol and methanol as a way to monitor recent alcohol consumption. During detoxification of alcohol-dependent subjects, ethanol persisted longer in urine than in breath or plasma. Blood and urinary methanol remained increased for 2-6 h after blood ethanol had returned to background concentrations, whereas 5HTOL/5HIAA remained increased for 6-15 h. In healthy volunteers who had ingested alcohol (range 3-98 g) the previous afternoon or evening, 87% (for men) and 59% (for women) of all drinking occasions exceeding 7 g of alcohol were identified by an increased 5HTOL/5HIAA in the first morning urine void. This compared with 32% and 12%, respectively, identified by analysis of ethanol (>200 micromol/L). No gender difference in the excretion pattern of 5HTOL/5HIAA was seen. The results demonstrate that 5HTOL/5HIAA provides a specific and more sensitive method to detect recent alcohol consumption than does ethanol or methanol.

DISTURBED URINARY EXCRETION PATTERN OF OESTROGENS IN NEWBORNS WITH CONGENITAL DISLOCATION OF THE HIP

1961 ◽  
Vol 37 (3) ◽  
pp. 423-426 ◽  
Author(s):  
L. Andrén ◽  
N. E. Borglin
Keyword(s):  
Urinary Excretion ◽  
Congenital Dislocation ◽  
Excretion Pattern ◽  
Dislocation Of The Hip

ABSTRACT There is an increased excretion of oestradiol-17β and oestrone in newborns with congenital dislocation of the hip. This increase is ascribed to an inborn and probably hereditary error of metabolism.

The Urinary Excretion of 1-Methylimidazole-4-Acetic Acid, a Histamine Metabolite, by Healthy and Asthmatic Children

1973 ◽  
Vol 45 (2) ◽  
pp. 193-198
Author(s):  
Hazel Thom ◽  
Joyce E. Richardson ◽  
R. G. Mitchell
Keyword(s):  
Acetic Acid ◽  
Urinary Excretion ◽  
Statistical Difference ◽  
Control Group ◽  
Histamine Metabolism ◽  
Restricted Diet ◽  
Asthmatic Children ◽  
Histamine Metabolite ◽  
Methylation Pathway

1. The excretion of 1-methylimidazole-4-acetic acid (MeIAA) in urine was measured in three groups of asthmatic children (acute-symptoms, quiescent and steroid-treated) and a control group of hospitalized non-asthmatic children all on the same restricted diet. 2. There was no statistical difference between the excretion of MeIAA in urine in any of the groups. There was therefore no evidence of impairment of the methylation pathway of histamine metabolism in asthma.

THE URINARY EXCRETION PATTERN OF TRACE METALS IN DIABETES MELLITUS

1962 ◽  
Vol 244 (3) ◽  
pp. 282-289 ◽  
Author(s):  
Lawrence E. Meltzer ◽  
Julia Rutman ◽  
Philip George ◽  
Robert Rutman ◽  
J Roderick Kitchell
Keyword(s):  
Diabetes Mellitus ◽  
Trace Metals ◽  
Urinary Excretion ◽  
Excretion Pattern

TYROSINOSIS: A PATIENT WITHOUT LIVER OR RENAL DISEASE

PEDIATRICS ◽  
1971 ◽  
Vol 48 (3) ◽  
pp. 393-400
Author(s):  
James L. Holston ◽  
Harvey L. Levy ◽  
Gary A. Tomlin ◽  
Ruby J. Atkins ◽  
T. H. Patton ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Mental Retardation ◽  
Renal Disease ◽  
Urinary Excretion ◽  
Hepatic Disease ◽  
Severe Mental Retardation ◽  
Primary Defect ◽  
Biochemical Evidence ◽  
Tyrosine Metabolism ◽  
Plasma Tyrosine

A child institutionalized because of severe mental retardation was found to have biochemical evidence of tyrosinosis. He has had no evidence of hepatic disease. In addition, he has had no hyperaminoaciduria, glycosuria, or rickets. Plasma tyrosine concentrations while he was on a regular diet ranged from 16.0 to 25.6 mg per 100 ml. Urinary excretion of tyrosine and tyrosine metabolites, including p-hydroxyphenylpyruvic acid, p-hydroxyphenyllactic acid, and p-hydroxyphenyl-acetic acid, were markedly increased. This patient appears to have a primary defect in tyrosine metabolism and represents individuals with persisting tyrosinemia and tyrosyluria in whom no hepatorenal disease is found.

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