A validated RP-HPLC method for the determination of rosuvastatin in presence of sacubitril/valsartan in rat plasma: Application to in vivo evaluation of OATP-mediated drug interaction potential between rosuvastatin and sacubitril/valsartan

2018 ◽  
Vol 143 ◽  
pp. 31-38 ◽  
Author(s):  
Bahia Abbas Moussa ◽  
Hanaa M.A. Hashem ◽  
Marianne Alphonse Mahrouse ◽  
Sally Tarek Mahmoud
Keyword(s):  
Drug Interaction ◽  
Interaction Potential ◽  
Rat Plasma ◽  
Hplc Method ◽  
In Vivo Evaluation ◽  
Rp Hplc

Ecofriendly chromatographic methods for determination of co-prescribed drugs, olanzapine and metformin, in rat plasma

Bioanalysis ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 597-613
Author(s):  
Aml A Emam ◽  
Neven M Habib ◽  
Hamada M Mahmoud ◽  
Nada S Abdelwhab ◽  
Maha M Abdelrahman
Keyword(s):  
Pharmaceutical Preparation ◽  
Rat Plasma ◽  
Reversed Phase ◽  
Hplc Method ◽  
Hypoglycemic Agent ◽  
Chromatographic Methods ◽  
Rp Hplc ◽  
Development And Validation ◽  
Developing System

Background: Olanzapine (OLZ) is one of most recommended drugs for the treatment of schizophrenia while metformin (MET) is the most commonly used hypoglycemic agent. Aim: Development and validation of two green, sensitive and accurate chromatographic methods for the simultaneous determination of OLZ along with the co-prescribed, MET. Materials & methods: TLC-densitometric method with a developing system consisting of methylene chloride:methanol:ethyl acetate:triethylamine (4:4:5:0.1, by volume) and a reversed-phase (RP)-HPLC method where the chromatographic separation was performed using ethanol:water mixture (50: 50, v/v) as a mobile phase. Results: TLC-densitometric method had linearity over concentration ranges of 160–4000 ng/band for OLZ and 150–4500 ng/band for MET, while RP-HPLC method was linear and validated over concentration range of 300–20000 ng/ml for OLZ and MET. Conclusion: Pharmacokinetic study was successfully performed and suggested the possibility of co-administration of MET with OLZ and their further formulation in one pharmaceutical preparation to enhance patient’s compliance.

A simplified and sensitive validated RP-HPLC method for determination of temozolomide in rat plasma and its application to a pharmacokinetic study

2018 ◽  
Vol 41 (10) ◽  
pp. 692-697 ◽  
Author(s):  
Archana Khosa ◽  
Kowthavarapu V. Krishna ◽  
Ranendra N. Saha ◽  
Sunil K. Dubey ◽  
Satish Reddi
Keyword(s):  
Pharmacokinetic Study ◽  
Rat Plasma ◽  
Hplc Method ◽  
Rp Hplc

scholarly journals Development of a new HPLC method for wogonin in rat plasma: Compatibility of standard and test samples

2017 ◽  
Vol 67 (3) ◽  
pp. 373-384 ◽  
Author(s):  
Jumah Masoud Mohammad Salmani ◽  
Xue Wu ◽  
Joe Antony Jacob ◽  
Rong Fu ◽  
Baoan Chen
Keyword(s):  
Standard Sample ◽  
Drug Distribution ◽  
Rat Plasma ◽  
Hplc Method ◽  
Standard Samples ◽  
Factors Affecting ◽  
Highly Hydrophobic ◽  
Plasma Compatibility

AbstractIn the current paper, an HPLC/UV method was developed and validated for determination of wogonin in plasma. Considerable attention was paid to the preparation of standard samples and factors affecting drug distribution. A preparation procedure was devised to simulate the conditions the drug is expected to experiencein vivowhile pointing to the shortcomings of previously published methods. The method was validated according to the FDA regulations and showed to be highly efficient and capable of extracting the drug and IS from the plasma accurately and precisely within the specified range of 50–500 ng mL−1. Further, the standard sample preparation of this method can be used as a guideline for other methods, particularly when highly hydrophobic drugs with considerable protein binding are involved and could be valuable in the field of bioanalysis to improve the reliability of methods.

scholarly journals Development of RP-HPLC method for simultaneous determination of docetaxel and curcumin in rat plasma: Validation and stability

2017 ◽  
Vol 12 (1) ◽  
pp. 105-113 ◽  
Author(s):  
Dong Wuk Kim ◽  
Abid Mehmood Yousaf ◽  
Dong Xun Li ◽  
Jong Oh Kim ◽  
Chul Soon Yong ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Rat Plasma ◽  
Hplc Method ◽  
Rp Hplc

scholarly journals Development and Validation of RP–HPLC Method for Estimation of Curcumin from Nanocochleates and Its Application in in–vivo Pharmacokinetic Study

2020 ◽  
Vol 67 (4) ◽  
pp. 110-1110
Author(s):  
Sameer Nadaf ◽  
Suresh Killedar
Keyword(s):  
Rat Plasma ◽  
Hplc Method ◽  
Pharmacokinetic Parameters ◽  
Statistical Control ◽  
Rp Hplc ◽  
Relative Standard ◽  
Validation Parameters ◽  
Limit Of Quantitation ◽  
Development And Validation

A reliable RP-HPLC analytical method with UV detection at 421 nm was developed and validated for the quantitative determination of curcumin from rat plasma after oral administration of curcumin loaded nanocochleates (CU-NC) to rats. The chromatographic separation was performed on HIQ SIL, C18 (250 mm × 4.6 mm) column using methanol and water (80:20 v/v) as mobile phase, at 1.0 mL/min flow rate. Validation parameters included linearity, accuracy, precision, and limit of quantitation and detection. Good linearity was obtained over the range of 2.5–100 μg/mL (R2 = 0.9979) of curcumin. The developed HPLC method was precise, with <2% relative standard deviation. Accuracy, stability, and robustness studies were also found to be acceptable. Bland-Altman plot showed an acceptable repeatability coefficient. The method was under statistical control, revealed by a control chart. After CU–NC administration, pharmacokinetic parameters i.e. Cmax, AUC0-∞, and AUMC0-∞, were observed to be 97.69 ± 10.84 μg/mL, 1402.77 ± 9.67 (μg/mL) ∙ h, and 35140.16 ± 14.67 (μg/mL) ∙ h2, respectively. This simple and precise method can be effectively implemented for routine analysis.

Sign in / Sign up

Export Citation Format

Share Document