Comparative analysis of the immune response of the wax moth Galleria mellonella after infection with the fungi Cordyceps militaris and Metarhizium robertsii

2020 ◽  
Vol 141 ◽  
pp. 103995 ◽  
Author(s):  
Vadim Yu Kryukov ◽  
Natalia A. Kryukova ◽  
Oksana G. Tomilova ◽  
Yana Vorontsova ◽  
Ekaterina Chertkova ◽  
...  
Keyword(s):  
Immune Response ◽  
Comparative Analysis ◽  
Galleria Mellonella ◽  
Cordyceps Militaris ◽  
Metarhizium Robertsii ◽  
Wax Moth

scholarly journals The effect of cold shock on the immune response of the greater wax moth Galleria mellonella after infection with entomopathogenic bacteria Bacillus thuringiensis / Wpływ szoku zimna na odpowiedź immunologiczną barciaka większego Galleria mellonella po zakażeniu bakterią entomopatogenną Bacillus thuringiensis

2015 ◽  
Vol 69 (2) ◽  
Author(s):  
Iwona Wojda ◽  
Paulina Taszłow ◽  
Teresa Jakubowicz
Keyword(s):  
Immune Response ◽  
Bacillus Thuringiensis ◽  
Cold Shock ◽  
Galleria Mellonella ◽  
Multifunctional Protein ◽  
Entomopathogenic Bacteria ◽  
Greater Wax Moth ◽  
Short Period ◽  
Defence Proteins ◽  
Wax Moth

AbstractInsect immune system consists of only innate mechanisms relied on cellular and humoral branches. Many defence proteins and peptides exist or appear in response to infection in insect’s hemolymph. The interaction between the infected host and the entomopathogen occurs in the conditions of external environment. In this work the greater wax moth larvae of Galleria mellonella were subjected to a temperature of 120C for a short period of time, directly before infection with entomopathogenic bacteria Bacillus thuringiensis. It appeared that the induction of the immune response was higher in cold-shocked animals than in larvae permanently reared at the optimal temperature of 28 0C. This enhanced immune response was manifested as higher antibacterial and lysozyme-type activity detected in full hemolymph, and as a higher level of peptides of molecular weight below 10 kDa having antibacterial activity. Moreover, other changes in the contents of proteins in the hemolymph were observed. These changes concerned inter alia apolipophorin III, the multifunctional protein of immune significance. Its level was higher in the hemolymph of animals pre-exposed to cold shock than in nonshocked, infected ones. Altogether our results indicate that the interdependence mechanisms occur between cold shock and the immune response.

scholarly journals The effect of entomopathogenic fungal culture filtrate on the immune response of the greater wax moth, Galleria mellonella

2017 ◽  
Vol 100 ◽  
pp. 82-92 ◽  
Author(s):  
Louise Mc Namara ◽  
James C. Carolan ◽  
Christine T. Griffin ◽  
David Fitzpatrick ◽  
Kevin Kavanagh
Keyword(s):  
Immune Response ◽  
Culture Filtrate ◽  
Galleria Mellonella ◽  
Fungal Culture ◽  
Greater Wax Moth ◽  
Wax Moth

scholarly journals Production of Cordyceps militaris metabolites in infected wax moth Galleria mellonella L. larvae

2019 ◽  
Vol 17 (1) ◽  
pp. 373-377
Author(s):  
M. V. Tyurin ◽  
T. I. Orlova ◽  
O. G. Tomilova ◽  
E. I. Saveleva ◽  
V. Yu. Kryukov ◽  
...  
Keyword(s):  
Galleria Mellonella ◽  
Cordyceps Militaris ◽  
Wax Moth

scholarly journals Culture Degeneration Reduces Sex-Related Gene Expression, Alters Metabolite Production and Reduces Insect Pathogenic Response in Cordyceps militaris

2021 ◽  
Vol 9 (8) ◽  
pp. 1559
Author(s):  
Peter A. D. Wellham ◽  
Abdul Hafeez ◽  
Andrej Gregori ◽  
Matthias Brock ◽  
Dong-Hyun Kim ◽  
...  
Keyword(s):  
Secondary Metabolite ◽  
Galleria Mellonella ◽  
Cordyceps Militaris ◽  
Insect Immunity ◽  
Pharmacological Activities ◽  
Metabolite Production ◽  
Expression Of Genes ◽  
Greater Wax Moth ◽  
Wax Moth

Cordyceps militaris is an entomopathogenic ascomycete, known primarily for infecting lepidopteran larval (caterpillars) and pupal hosts. Cordycepin, a secondary metabolite produced by this fungus has anti-inflammatory properties and other pharmacological activities. However, little is known about the biological role of this adenosine derivate and its stabilising compound pentostatin in the context of insect infection the life cycle of C. militaris. During repeated subcultivation under laboratory conditions a degeneration of C. militaris marked by decreasing levels of cordycepin production can occur. Here, using degenerated and parental control strains of an isolate of C. militaris, we found that lower cordycepin production coincides with the decline in the production of various other metabolites as well as the reduced expression of genes related to sexual development. Additionally, infection of Galleria mellonella (greater wax moth) caterpillars indicated that cordycepin inhibits the immune response in host haemocytes. Accordingly, the pathogenic response to the degenerated strain was reduced. These data indicate that there are simultaneous changes in sexual reproduction, secondary metabolite production, insect immunity and infection by C. militaris. This study may have implications for biological control of insect crop pests by fungi.

scholarly journals Cloning and expression of an inhibitor of microbial metalloproteinases from insects contributing to innate immunity

2004 ◽  
Vol 382 (1) ◽  
pp. 315-322 ◽  
Author(s):  
Anja CLERMONT ◽  
Marianne WEDDE ◽  
Volkhard SEITZ ◽  
Lars PODSIADLOWSKI ◽  
Dido LENZE ◽  
...  
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Galleria Mellonella ◽  
Cloning And Expression ◽  
Tissue Inhibitors Of Metalloproteinases ◽  
Pcr Analysis ◽  
Humoral Immune ◽  
Greater Wax Moth ◽  
Natural Inhibitors ◽  
Wax Moth

The first IMPI (inhibitor of metalloproteinases from insects) was identified in the greater wax moth, Galleria mellonella [Wedde, Weise, Kopacek, Franke and Vilcinskas (1998) Eur. J. Biochem. 255, 535–543]. Here we report cloning and expression of a cDNA coding for this IMPI. The IMPI mRNA was identified among the induced transcripts from a subtractive and suppressive PCR analysis after bacterial challenge of G. mellonella larvae. Induced expression of the IMPI during a humoral immune response was confirmed by real-time PCR, which documented up to 500 times higher amounts of IMPI mRNA in immunized larvae in comparison with untreated ones. The IMPI sequence shares no similarity with those of tissue inhibitors of metalloproteinases or other natural inhibitors of metalloproteinases, and the recombinant IMPI specifically inhibits thermolysin-like metalloproteinases, but not matrix metalloproteinases. These results support the hypothesis that the IMPI represents a novel type of immune-related protein which is induced and processed during the G. mellonella humoral immune response to inactivate pathogen-associated thermolysin-like metalloproteinases.

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