scholarly journals Cloning and expression of an inhibitor of microbial metalloproteinases from insects contributing to innate immunity

2004 ◽  
Vol 382 (1) ◽  
pp. 315-322 ◽  
Author(s):  
Anja CLERMONT ◽  
Marianne WEDDE ◽  
Volkhard SEITZ ◽  
Lars PODSIADLOWSKI ◽  
Dido LENZE ◽  
...  
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Galleria Mellonella ◽  
Cloning And Expression ◽  
Tissue Inhibitors Of Metalloproteinases ◽  
Pcr Analysis ◽  
Humoral Immune ◽  
Greater Wax Moth ◽  
Natural Inhibitors ◽  
Wax Moth

The first IMPI (inhibitor of metalloproteinases from insects) was identified in the greater wax moth, Galleria mellonella [Wedde, Weise, Kopacek, Franke and Vilcinskas (1998) Eur. J. Biochem. 255, 535–543]. Here we report cloning and expression of a cDNA coding for this IMPI. The IMPI mRNA was identified among the induced transcripts from a subtractive and suppressive PCR analysis after bacterial challenge of G. mellonella larvae. Induced expression of the IMPI during a humoral immune response was confirmed by real-time PCR, which documented up to 500 times higher amounts of IMPI mRNA in immunized larvae in comparison with untreated ones. The IMPI sequence shares no similarity with those of tissue inhibitors of metalloproteinases or other natural inhibitors of metalloproteinases, and the recombinant IMPI specifically inhibits thermolysin-like metalloproteinases, but not matrix metalloproteinases. These results support the hypothesis that the IMPI represents a novel type of immune-related protein which is induced and processed during the G. mellonella humoral immune response to inactivate pathogen-associated thermolysin-like metalloproteinases.

scholarly journals The effect of cold shock on the immune response of the greater wax moth Galleria mellonella after infection with entomopathogenic bacteria Bacillus thuringiensis / Wpływ szoku zimna na odpowiedź immunologiczną barciaka większego Galleria mellonella po zakażeniu bakterią entomopatogenną Bacillus thuringiensis

2015 ◽  
Vol 69 (2) ◽  
Author(s):  
Iwona Wojda ◽  
Paulina Taszłow ◽  
Teresa Jakubowicz
Keyword(s):  
Immune Response ◽  
Bacillus Thuringiensis ◽  
Cold Shock ◽  
Galleria Mellonella ◽  
Multifunctional Protein ◽  
Entomopathogenic Bacteria ◽  
Greater Wax Moth ◽  
Short Period ◽  
Defence Proteins ◽  
Wax Moth

AbstractInsect immune system consists of only innate mechanisms relied on cellular and humoral branches. Many defence proteins and peptides exist or appear in response to infection in insect’s hemolymph. The interaction between the infected host and the entomopathogen occurs in the conditions of external environment. In this work the greater wax moth larvae of Galleria mellonella were subjected to a temperature of 120C for a short period of time, directly before infection with entomopathogenic bacteria Bacillus thuringiensis. It appeared that the induction of the immune response was higher in cold-shocked animals than in larvae permanently reared at the optimal temperature of 28 0C. This enhanced immune response was manifested as higher antibacterial and lysozyme-type activity detected in full hemolymph, and as a higher level of peptides of molecular weight below 10 kDa having antibacterial activity. Moreover, other changes in the contents of proteins in the hemolymph were observed. These changes concerned inter alia apolipophorin III, the multifunctional protein of immune significance. Its level was higher in the hemolymph of animals pre-exposed to cold shock than in nonshocked, infected ones. Altogether our results indicate that the interdependence mechanisms occur between cold shock and the immune response.

scholarly journals The effect of entomopathogenic fungal culture filtrate on the immune response of the greater wax moth, Galleria mellonella

2017 ◽  
Vol 100 ◽  
pp. 82-92 ◽  
Author(s):  
Louise Mc Namara ◽  
James C. Carolan ◽  
Christine T. Griffin ◽  
David Fitzpatrick ◽  
Kevin Kavanagh
Keyword(s):  
Immune Response ◽  
Culture Filtrate ◽  
Galleria Mellonella ◽  
Fungal Culture ◽  
Greater Wax Moth ◽  
Wax Moth

Cloning and expression of male-specific protein (MSP) from the hemolymph of greater wax moth,Galleria mellonella L.

2003 ◽  
Vol 54 (3) ◽  
pp. 110-120 ◽  
Author(s):  
Jikhyon Han ◽  
Chang Seok Lee ◽  
Chi Young Yun ◽  
Bong Hee Lee ◽  
Young Gyu Ko ◽  
...  
Keyword(s):  
Galleria Mellonella ◽  
Specific Protein ◽  
Cloning And Expression ◽  
Greater Wax Moth ◽  
Male Specific ◽  
Wax Moth
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