OmpW expressed by recombinant Lactobacillus casei elicits protective immunity against Aeromonas veronii in common carp

2019 ◽  
Vol 133 ◽  
pp. 103552 ◽  
Author(s):  
Lei Zhang ◽  
Zhenxing Li ◽  
Ying Li ◽  
Jiaxin Tian ◽  
Kaixiang Jia ◽  
...  
Keyword(s):  
Common Carp ◽  
Protective Immunity ◽  
Lactobacillus Casei ◽  
Aeromonas Veronii

scholarly journals Oral Administration of Lactobacillus Casei Expressing Flagellin A Protein Confers Effective Protection against Aeromonas Veronii in Common Carp, Cyprinus Carpio

2019 ◽  
Vol 21 (1) ◽  
pp. 33
Author(s):  
Jia-Xin Tian ◽  
Yuan-Huan Kang ◽  
Guo-Sheng Chu ◽  
Hong-Jian Liu ◽  
Yi-Di Kong ◽  
...  
Keyword(s):  
Immune System ◽  
Immune Responses ◽  
Common Carp ◽  
Lactobacillus Casei ◽  
Survival Rates ◽  
High Serum ◽  
Mucosal Vaccine ◽  
Economic Losses ◽  
Cell Immune Response ◽  
Aeromonas Veronii

Aeromonas veronii is a pathogen capable of infecting humans, livestock and aquatic animals, resulting in serious economic losses. In this study, two recombinant Lactobacillus casei expressing flagellin A (FlaA) of A. veronii, Lc-pPG-1-FlaA (surface-displayed) and Lc-pPG-2-FlaA (secretory) were constructed. The immune responses in fish administered with recombinant L. casei were evaluated. The two recombinant L. casei were orally administered to common carp, which stimulated high serum IgM and induced higher ACP, AKP, SOD and LYZ activity. Using qRT-PCR, the expression of IL-10, IL-8, IL-1β, TNF-α and IFN-γ in the tissue of fish immunized with recombinant L. casei was significantly (p < 0.05) upregulated, which indicated that recombinant L. casei could activate the innate immune system to trigger the cell immune response and inflammatory response. Furthermore, recombinant L. casei was able to survive the intestinal environment and colonize in intestine mucosal. The study showed that after being challenged by A. veronii, fish administered with Lc-pPG-1-FlaA (70%) and Lc-pPG-2-FlaA (50%) had higher survival rates compared to Lc-pPG and PBS, indicating that recombinant L. casei might prevent A. veronii infection by activating the immune system to trigger immune responses. We demonstrated that flagellin as an antigen of vaccine, is acceptable for preventing A. veronii infection in fish. The recombinant L. casei expressing FlaA may be a novel mucosal vaccine for treating and controlling A. veronii.

Prevention of gastrointestinal infection using immunobiological methods with milk fermented withLactobacillus caseiandLactobacillus acidophilus

1990 ◽  
Vol 57 (2) ◽  
pp. 255-264 ◽  
Author(s):  
Gabriela Perdigon ◽  
Maria E. Nader de Macias ◽  
Susana Alvarez ◽  
Guillermo Oliver ◽  
Aida A. Pesce de Ruiz Holgado
Keyword(s):  
Protective Immunity ◽  
Lactobacillus Casei ◽  
Fermented Milk ◽  
Oral Infection ◽  
Gastrointestinal Infection ◽  
Mucosal Tissue ◽  
Intestinal Fluid ◽  
Pathogen Colonization ◽  
The Individual ◽  
Effect Of Feeding

SummaryThe protective effect of feeding milk fermented with a mixture ofLactobacillus caseisp. andLb. acidophilussp. againstSalmonella typhimuriuminfection in mice was compared with that obtained feeding milks fermented with these microorganisms individually. The survival rate obtained after oral infection withSal. typhimuriumwas 100% in mice pretreated by feeding during 8 d with the mixture ofLb. caseiandLb. acidophilusfermented milks. Similar treatments with the individual milks were ineffective. Moreover, mice became more susceptible to infection withSal. typhimuriumafter such treatment. The colonization of liver and spleen with the pathogen was markedly inhibited by the pretreatment with the mixture of fermented milk, while such inhibition was not observed using theLb. caseiandLb. acidophilusmilks. The highest levels of anti-salmonellae antibodies in serum and in intestinal fluid were found in the group of mice fed with the mixture and withLb. caseifermented milk respectively. However, this latter milk was not effective in protecting againstSal. typhimurium. When the mice were first infected withSal. typhimuriumand then fed with the mixture of fermented milks, pathogen colonization was not prevented. The results suggest that the augmentation of resistance to salmonellae caused by the treatment withLb. casei-+Lb. acidophilus-fermented milk was due to the anti-salmonellae protective immunity mainly mediated by the mucosal tissue. Milk fermented with this mixture could be used as an immunobiological method to prevent gastrointestinal infection.

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