Correlation and in vitro mechanism of bactericidal activity on E. coli with whey protein isolate during ultrasonic treatment

2018 ◽  
Vol 115 ◽  
pp. 154-158 ◽  
Author(s):  
Liyan Liu ◽  
Ruirui Xu ◽  
Lin Li ◽  
Bing Li ◽  
Xia Zhang ◽  
...  
Keyword(s):  
Whey Protein ◽  
Bactericidal Activity ◽  
Ultrasonic Treatment ◽  
Whey Protein Isolate ◽  
Protein Isolate ◽  
E Coli

scholarly journals Freeze-Dried Curdlan/Whey Protein Isolate-Based Biomaterial as Promising Scaffold for Matrix-Associated Autologous Chondrocyte Transplantation—A Pilot In-Vitro Study

Cells ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 282
Author(s):  
Katarzyna Klimek ◽  
Marta Tarczynska ◽  
Wieslaw Truszkiewicz ◽  
Krzysztof Gaweda ◽  
Timothy E. L. Douglas ◽  
...  
Keyword(s):  
Pilot Study ◽  
Whey Protein ◽  
Whey Protein Isolate ◽  
Protein Isolate ◽  
Human Chondrocytes ◽  
Autologous Chondrocyte Transplantation ◽  
Chondrocyte Transplantation ◽  
Freeze Dried ◽  
Autologous Chondrocyte

The purpose of this pilot study was to establish whether a novel freeze-dried curdlan/whey protein isolate-based biomaterial may be taken into consideration as a potential scaffold for matrix-associated autologous chondrocyte transplantation. For this reason, this biomaterial was initially characterized by the visualization of its micro- and macrostructures as well as evaluation of its mechanical stability, and its ability to undergo enzymatic degradation in vitro. Subsequently, the cytocompatibility of the biomaterial towards human chondrocytes (isolated from an orthopaedic patient) was assessed. It was demonstrated that the novel freeze-dried curdlan/whey protein isolate-based biomaterial possessed a porous structure and a Young’s modulus close to those of the superficial and middle zones of cartilage. It also exhibited controllable degradability in collagenase II solution over nine weeks. Most importantly, this biomaterial supported the viability and proliferation of human chondrocytes, which maintained their characteristic phenotype. Moreover, quantitative reverse transcription PCR analysis and confocal microscope observations revealed that the biomaterial may protect chondrocytes from dedifferentiation towards fibroblast-like cells during 12-day culture. Thus, in conclusion, this pilot study demonstrated that novel freeze-dried curdlan/whey protein isolate-based biomaterial may be considered as a potential scaffold for matrix-associated autologous chondrocyte transplantation.

scholarly journals Effect of inulin, medium-chain triglycerides and whey protein isolate on stability and in vitro digestibility of enteral nutrition formulas

2020 ◽  
Vol 40 (4) ◽  
pp. 854-863 ◽  
Author(s):  
Mariana Wanessa Santana de SOUZA ◽  
Evelyn de Souza Oliveira LOPES ◽  
Gustavo Pereira COSENZA ◽  
Verônica Ortiz ALVARENGA ◽  
Renata Adriana LABANCA ◽  
...  
Keyword(s):  
Enteral Nutrition ◽  
Whey Protein ◽  
Whey Protein Isolate ◽  
Protein Isolate ◽  
In Vitro Digestibility ◽  
Medium Chain Triglycerides ◽  
Medium Chain
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