New insights into the understanding of flow dynamics in an in vitro model for abdominal aortic aneurysms

2013 ◽  
Vol 35 (6) ◽  
pp. 800-809 ◽  
Author(s):  
Valérie Deplano ◽  
Clark Meyer ◽  
Carine Guivier-Curien ◽  
Eric Bertrand
Keyword(s):  
In Vitro Model ◽  
Abdominal Aortic Aneurysms ◽  
Flow Dynamics ◽  
Aortic Aneurysms ◽  
Abdominal Aortic ◽  
Vitro Model

scholarly journals MiR-191 as a Key Molecule in Aneurysmal Aortic Remodeling

Biomolecules ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1611
Author(s):  
Sabina Lichołai ◽  
Dorota Studzińska ◽  
Hanna Plutecka ◽  
Tomasz Gubała ◽  
Wojciech Szczeklik ◽  
...  
Keyword(s):  
Complex Disease ◽  
Positive Family History ◽  
Significant Risk ◽  
Significant Risk Factor ◽  
Abdominal Aortic Aneurysms ◽  
Aortic Aneurysms ◽  
Control Group ◽  
Abdominal Aortic ◽  
Vitro Model

Abdominal aortic aneurysms (AAA) are a complex disease with an unclear pathomechanism. A positive family history is emphasized as a significant risk factor, and a nonspecific model of inheritance suggests participation of epigenetic regulation in the pathogenesis of this disease. Past studies have implicated microRNAs in the development of AAA; therefore in this project, we measured miR-191 levels in AAA patients and compared them with a control group. We found that miR-191 levels were significantly elevated in aneurysmal patients, although this did not correlate with the available clinical data. We then developed an in vitro model where, using cells with an endothelial phenotype, we determined the effect of miR-191 on the transcriptome using RNA sequencing. Subsequent pathway analysis established that some of the perturbations mediated by miR-191 can be explained by several processes which have long been observed and described in literature as accompanying the development of abdominal aortic aneurysms.

Abstract 116: Simvastatin Affects Monocyte Adhesion and Infiltrative Activity in Patients With Abdominal Aortic Aneurysms

2014 ◽  
Vol 34 (suppl_1) ◽  
Author(s):  
Kiana M Samadzadeh ◽  
Anthony Nguyen ◽  
Kevin C Chun ◽  
Eugene S Lee
Keyword(s):  
Abdominal Aortic Aneurysms ◽  
Statin Treatment ◽  
Aortic Aneurysms ◽  
Monocyte Adhesion ◽  
Abdominal Aortic ◽  
Monocyte Cell ◽  
High Concentrations ◽  
Statin Drugs ◽  
Monocyte Adherence

Purpose: The pleiotropic effects of statin drugs on reducing inflammation have been well regarded in decreasing AAA expansion. We hypothesize that increased monocyte activity plays a central role in AAA formation and expansion. This study examines whether statins can prevent monocyte cell adhesion, transmigration, and matrix metalloproteinase (MMP) and inhibitor (TIMP) concentrations in AAA patients compared to non-AAA patients. Methods: Peripheral blood was collected for monocyte and serum isolation from control (n=4) and AAA (n=8) patients. Monocyte adhesion and transmigration were assessed under untreated, statin treated, and statin + mevalonate (statin inhibitor) treated conditions in vitro. Luminex assays determined MMP and TIMP concentrations from cell culture and patient serum. Results: Untreated AAA patient monocytes showed higher levels of adhesion (p=0.05) and transmigration (p=0.04) compared to control subjects (Figure 1A & 1B). Statin treatment caused a decrease in AAA monocyte adherence to the endothelium (p=0.03) and high concentrations of mevalonate reversed statin treatment effects (p=0.04) (Figure 1A). A similar trend was noted in monocyte transmigration (Figure 1B). Higher concentrations of MMP-9 were found in AAA patient serum compared to controls (p=0.01) (Figure 1C). TIMP-4 concentration were decreased in AAA patients compared to controls (p=0.02) (Figure 1D). Conclusions: Statins reduce monocyte interaction with the endothelium in vitro, leading to decreased levels of MMP-9 and increased levels of TIMP-4, implying a possible mechanism by which statins reduce AAA expansion.

A Review of the In Vivo and In Vitro Biomechanical Behavior and Performance of Postoperative Abdominal Aortic Aneurysms and Implanted Stent-Grafts

2008 ◽  
Vol 15 (4) ◽  
pp. 468-484 ◽  
Author(s):  
Timothy J. Corbett ◽  
Anthony Callanan ◽  
Liam G. Morris ◽  
Barry J. Doyle ◽  
Pierce A. Grace ◽  
...  
Keyword(s):  
Abdominal Aortic Aneurysms ◽  
Aortic Aneurysms ◽  
Stent Grafts ◽  
Biomechanical Behavior ◽  
Abdominal Aortic ◽  
And Performance

scholarly journals 176-02: A Novel In-Vitro Model to Study Flow Dynamics in the Human Right Heart

EP Europace ◽  
2016 ◽  
Vol 18 (suppl_1) ◽  
pp. i117-i117
Author(s):  
Utku Gulan ◽  
Ardan Saguner ◽  
Deniz Akdis ◽  
Corinna Brunckhorst ◽  
Alexander Gotschy ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Flow Dynamics ◽  
Human Right ◽  
Right Heart ◽  
Vitro Model

scholarly journals Impact of Yttrium-90 Microsphere Density, Flow Dynamics, and Administration Technique on Spatial Distribution: Analysis Using an In Vitro Model

2017 ◽  
Vol 28 (2) ◽  
pp. 260-268.e2 ◽  
Author(s):  
Marcus Caine ◽  
Michael S. McCafferty ◽  
Scott McGhee ◽  
Pedro Garcia ◽  
Wayne M. Mullett ◽  
...  
Keyword(s):  
Spatial Distribution ◽  
In Vitro Model ◽  
Flow Dynamics ◽  
Distribution Analysis ◽  
Density Flow ◽  
Vitro Model ◽  
Yttrium 90

The Development of Physiological Compliant Abdominal Aortic Aneurysm Models for In Vitro Flow Studies

2009 ◽  
Author(s):  
Timothy J. Corbett ◽  
Barry J. Doyle ◽  
Anthony Callanan ◽  
Tim M. McGloughlin
Keyword(s):  
Abdominal Aortic Aneurysm ◽  
Aortic Aneurysm ◽  
Material Properties ◽  
Abdominal Aortic Aneurysms ◽  
Aortic Aneurysms ◽  
Flow Loop ◽  
The Past ◽  
Abdominal Aortic

A vast amount of experimental research has been undertaken in the past decade to investigate different aspects of preoperative and postoperative abdominal aortic aneurysms (AAAs). Much of this research has been based on the use of mock arteries in an in vitro flow loop to mimic the behaviour of the abdominal aorta in vivo [1]. These models should be reproducible, have consistent material properties, consistent thickness and be physiological in behaviour.

Real-Time Measurement of Multi-Component Velocity Vectors Within Abdominal Aortic Aneurysms Using Echo PIV: Comparison of In Vitro and Computational Results

2007 ◽  
Author(s):  
Lingli Liu ◽  
Fuxing Zhang ◽  
Rui Wang ◽  
Robin Shandas
Keyword(s):  
Disease Process ◽  
Abdominal Aortic Aneurysms ◽  
The United States ◽  
Aneurysm Rupture ◽  
Aortic Aneurysms ◽  
Non Invasive ◽  
Abdominal Aortic ◽  
Component Velocity ◽  
Aneurysm Growth

Abdominal aortic aneurysms (AAAs) are localized balloon-shaped expansions commonly found in the infrarenal segment of the abdominal aorta, between the renal arteries and the iliac bifurcation. Abdominal aortic aneurysm rupture has been estimated to occur in as much as 3%–9% of the population, and represents the 13th leading cause of death in the United States, producing more than 10,000 deaths annually [1]. Thus, determining the significant factors for aneurysm growth and rupture has become an important clinical goal. From a biomechanical standpoint, AAA rupture risk is related to certain mechanical and hemodynamic factors such as localized flow fields and velocity patterns, and flow-induced stresses within the fluid and in the aneurysm structure [2]. Disturbed flow patterns at different levels have also been found to trigger responses within medial and adventitial layers by altering intercellular communication mechanisms. Thus, localized hemodynamics proximal, within and distal to AAA formations play an important role in modulating the disease process, and non-invasive and easy-to-implement methods to characterize and quantify these complex hemodynamics would be tremendously useful.

The Potential of AFX Iliac Extension in Abdominal Aortic Aneurysms with High Iliac Tortuosity

2021 ◽  
Author(s):  
Yuta Kikuchi ◽  
Norifumi Ohtani ◽  
Hiroyuki Kamiya
Keyword(s):  
Aortic Aneurysm ◽  
Circulatory System ◽  
Abdominal Aortic Aneurysms ◽  
Aortic Aneurysms ◽  
Abdominal Aortic ◽  
Silicon Tube ◽  
Underwater Camera ◽  
Aneurysm Repair ◽  
Aortic Aneurysm Repair

Abstract Background Recently, endovascular aortic aneurysm repair (EVAR) is the most common surgery for abdominal aortic aneurysm (AAA). However, iliac limb complications of EVAR often cause problems in patients with high iliac tortuosity. There is no difference of rate of iliac limb complication among EVAR devices, such as Excluder, Endurant, and Zenith in high iliac tortuosity. But there has been not reported about AFX. Objectives We studied AFX iliac extension as it is the only stent graft with an endoskeletal framework. This study aimed to evaluate the AFX iliac extension patency in a case in vitro and to use it in seven cases of AAA with high iliac tortuosity. Methods The silicon tube inserted in the AFX iliac extension was flexed at 30, 60, 90, and 120 degrees, and the lumen of the iliac extension was monitored using an underwater camera in the circulatory system. During the experiment, the Iwaki Bellows Pump (IWAKI CO., LTD., Tokyo, Japan) produced a pulsating flow. We used this in seven patients with AAA high iliac tortuosity cases between November 2018 and May 2019. Results If the silicon tube inserted in the AFX iliac extension was flexed at 60 and 120 degrees, the stent protruded into the lumen. However, the graft was dilated at all degrees. All seven patients with AFX iliac extension had no complications and a patent iliac artery. Conclusion The AFX iliac extension can reduce iliac limb complications in cases of high iliac tortuosity.

Pulsed and Color Doppler Analysis of Normal Carotid Bifurcation Flow Dynamics Using an In-Vitro Model

Angiology ◽  
1990 ◽  
Vol 41 (3) ◽  
pp. 241-247 ◽  
Author(s):  
Joseph F. Polak ◽  
Daniel H. O'Leary ◽  
William C. Quist ◽  
Mark A. Creager ◽  
Frank W. LoGerfo
Keyword(s):  
In Vitro Model ◽  
Color Doppler ◽  
Carotid Bifurcation ◽  
Flow Dynamics ◽  
Vitro Model ◽  
Doppler Analysis
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