Neurotrophic factor-α1 modulates NGF-induced neurite outgrowth through interaction with Wnt-3a and Wnt-5a in PC12 cells and cortical neurons

2015 ◽  
Vol 68 ◽  
pp. 222-233 ◽  
Author(s):  
Prabhuanand Selvaraj ◽  
Jane S.W. Huang ◽  
Alexander Chen ◽  
Nir Skalka ◽  
Rina Rosin-Arbesfeld ◽  
...  
Keyword(s):  
Neurite Outgrowth ◽  
Pc12 Cells ◽  
Neurotrophic Factor ◽  
Cortical Neurons

scholarly journals The Adaptor Protein SH2B3 (Lnk) Negatively Regulates Neurite Outgrowth of PC12 Cells and Cortical Neurons

PLoS ONE ◽  
2011 ◽  
Vol 6 (10) ◽  
pp. e26433 ◽  
Author(s):  
Tien-Cheng Wang ◽  
Hsun Chiu ◽  
Yu-Jung Chang ◽  
Tai-Yu Hsu ◽  
Ing-Ming Chiu ◽  
...  
Keyword(s):  
Neurite Outgrowth ◽  
Pc12 Cells ◽  
Cortical Neurons ◽  
Adaptor Protein

scholarly journals MiR-133b Promotes Neurite Outgrowth by Targeting RhoA Expression

2015 ◽  
Vol 35 (1) ◽  
pp. 246-258 ◽  
Author(s):  
Xiao Cheng Lu ◽  
Jin Yu Zheng ◽  
Lin Jun Tang ◽  
Bao Sheng Huang ◽  
Kai Li ◽  
...  
Keyword(s):  
Neurite Outgrowth ◽  
Signaling Pathway ◽  
Pc12 Cells ◽  
Cortical Neurons ◽  
Critical Role ◽  
Axon Growth ◽  
Akt Signaling ◽  
Neural Cells ◽  
Spinal Cord Regeneration ◽  
Akt Signaling Pathway

Background: MicroRNA-133b (miR-133b) has been shown to play a critical role in spinal cord regeneration. The aim of this study was to investigate the cellular role of miR-133b in neural cells. Methods: PC12 cells and primary cortical neurons (PCNs) were transfected with lenti-miR-133b, lenti-miR-133b inhibitor, plasmid-shRNA-RhoA, plasmid-RhoA and their negative controls. After 48 hours of transfection, the levels of proteins and mRNA or miRNA were evaluated by Western blotting and qRT-PCR, respectively. Moreover, the neurite outgrowth was analyzed by Image J. For pharmacological experiments, inhibitors of MEK1/2 kinase (PD98059), phosphoinositide-3 kinase (PI3K) (LY294002) and ROCK (Y27632) were added into the culture medium. Results: Overexpression of miR-133b in PC12 cells enhanced neurite outgrowth. Conversely, inhibition of miR-133b reduced neurite length. We further identified RhoA as a target and mediator of mir-133b for neurite extension by Western blot and knockdown experiment. Moreover, overexpression of RhoA could attenuate the neurite growth effects of miR-133b. Also, we observed that miR-133b activated MEK/ERK and PI3K/Akt signaling pathway by targeting RhoA. Finally, in PCNs, miR-133b also increased axon growth and attenuated axon growth restrictions from chondroitin sulfate proteoglycans (CSPG). Conclusions: In summary, our study suggested that miR-133b regulated neurite outgrowth via ERK1/2 and PI3K/Akt signaling pathway by RhoA suppression.

scholarly journals Etifoxine promotes glial-derived neurotrophic factor-induced neurite outgrowth in PC12 cells

2013 ◽  
Vol 8 (1) ◽  
pp. 75-80 ◽  
Author(s):  
XIANG ZHOU ◽  
XINHUA HE ◽  
BO HE ◽  
ZHAOWEI ZHU ◽  
CANBIN ZHENG ◽  
...  
Keyword(s):  
Neurite Outgrowth ◽  
Pc12 Cells ◽  
Neurotrophic Factor ◽  
Glial Derived Neurotrophic Factor

scholarly journals Sez-6 May Play an Important Role in Neurite Outgrowth through the PKCγ Signaling Pathways

2011 ◽  
Vol 66 (11-12) ◽  
pp. 614-620
Author(s):  
Jing Zhang ◽  
Jun Zhang ◽  
Youjun Wu ◽  
Yang Zhou ◽  
Zhihua Shao ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Neurite Outgrowth ◽  
Pc12 Cells ◽  
Cortical Neurons ◽  
Neuronal Development ◽  
Hairpin Rna ◽  
Kinase C ◽  
Shrna Plasmid ◽  
And Function

Seizure-related gene 6 (sez-6) was originally identified in a study of pentylenetetrazoletreated cortical neurons. Further studies on the structure and expression pattern suggested that Sez-6 may play an important role in neuronal development and function. In the present study, PC12 cells were used as a model to investigate the role of Sez-6 in neurite outgrowth. After a period of NGF treatment, the expression of Sez-6 in PC12 cells was increased. When Sez-6 expression was suppressed by the addition of an effective short hairpin RNA ( shRNA) plasmid, the neurite outgrowth was significantly inhibited. In addition, we detected the expression level of protein kinase Cγ (PKCγ), and found that the PKCγ protein level was reduced in the differentiated PC12 cells but increased in PC12 cells lacking Sez-6. Taken together, our results indicate that Sez-6 acts on the neurite outgrowth of PC12 cells likely through the PKCγ signaling pathways

Hyperforin mediates neurite outgrowth via TRPC6 channels in PC12 cells

2007 ◽  
Vol 40 (05) ◽  
Author(s):  
K Leuner ◽  
M Müller ◽  
V Kasanzki ◽  
C Harteneck ◽  
WE Müller
Keyword(s):  
Neurite Outgrowth ◽  
Pc12 Cells

Sesquiterpenoids of Illicium jiadifengpi and Their Effects on NGF-induced Neurite Outgrowth in PC12 Cells

2017 ◽  
Vol 35 (10) ◽  
pp. 1640-1643 ◽  
Author(s):  
Jifeng Liu ◽  
Xiaoyu Su ◽  
Feixia Hu ◽  
Jing Guo ◽  
Xu Song ◽  
...  
Keyword(s):  
Neurite Outgrowth ◽  
Pc12 Cells
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