Purification and Characterization of a 15 kDa Protein (p15) Produced byHelicosporiumThat Exhibits Distinct Effects on Neurite Outgrowth from Cortical Neurons and PC12 Cells

1996 ◽  
Vol 228 (1) ◽  
pp. 209-215 ◽  
Author(s):  
Toshihiko Hanada ◽  
Takaaki Sato ◽  
Manabu Arioka ◽  
Masakazu Uramoto ◽  
Makari Yamasaki
Keyword(s):  
Neurite Outgrowth ◽  
Pc12 Cells ◽  
Cortical Neurons ◽  
Purification And Characterization

Neurotrophic factor-α1 modulates NGF-induced neurite outgrowth through interaction with Wnt-3a and Wnt-5a in PC12 cells and cortical neurons

2015 ◽  
Vol 68 ◽  
pp. 222-233 ◽  
Author(s):  
Prabhuanand Selvaraj ◽  
Jane S.W. Huang ◽  
Alexander Chen ◽  
Nir Skalka ◽  
Rina Rosin-Arbesfeld ◽  
...  
Keyword(s):  
Neurite Outgrowth ◽  
Pc12 Cells ◽  
Neurotrophic Factor ◽  
Cortical Neurons

scholarly journals The Adaptor Protein SH2B3 (Lnk) Negatively Regulates Neurite Outgrowth of PC12 Cells and Cortical Neurons

PLoS ONE ◽  
2011 ◽  
Vol 6 (10) ◽  
pp. e26433 ◽  
Author(s):  
Tien-Cheng Wang ◽  
Hsun Chiu ◽  
Yu-Jung Chang ◽  
Tai-Yu Hsu ◽  
Ing-Ming Chiu ◽  
...  
Keyword(s):  
Neurite Outgrowth ◽  
Pc12 Cells ◽  
Cortical Neurons ◽  
Adaptor Protein

scholarly journals MiR-133b Promotes Neurite Outgrowth by Targeting RhoA Expression

2015 ◽  
Vol 35 (1) ◽  
pp. 246-258 ◽  
Author(s):  
Xiao Cheng Lu ◽  
Jin Yu Zheng ◽  
Lin Jun Tang ◽  
Bao Sheng Huang ◽  
Kai Li ◽  
...  
Keyword(s):  
Neurite Outgrowth ◽  
Signaling Pathway ◽  
Pc12 Cells ◽  
Cortical Neurons ◽  
Critical Role ◽  
Axon Growth ◽  
Akt Signaling ◽  
Neural Cells ◽  
Spinal Cord Regeneration ◽  
Akt Signaling Pathway

Background: MicroRNA-133b (miR-133b) has been shown to play a critical role in spinal cord regeneration. The aim of this study was to investigate the cellular role of miR-133b in neural cells. Methods: PC12 cells and primary cortical neurons (PCNs) were transfected with lenti-miR-133b, lenti-miR-133b inhibitor, plasmid-shRNA-RhoA, plasmid-RhoA and their negative controls. After 48 hours of transfection, the levels of proteins and mRNA or miRNA were evaluated by Western blotting and qRT-PCR, respectively. Moreover, the neurite outgrowth was analyzed by Image J. For pharmacological experiments, inhibitors of MEK1/2 kinase (PD98059), phosphoinositide-3 kinase (PI3K) (LY294002) and ROCK (Y27632) were added into the culture medium. Results: Overexpression of miR-133b in PC12 cells enhanced neurite outgrowth. Conversely, inhibition of miR-133b reduced neurite length. We further identified RhoA as a target and mediator of mir-133b for neurite extension by Western blot and knockdown experiment. Moreover, overexpression of RhoA could attenuate the neurite growth effects of miR-133b. Also, we observed that miR-133b activated MEK/ERK and PI3K/Akt signaling pathway by targeting RhoA. Finally, in PCNs, miR-133b also increased axon growth and attenuated axon growth restrictions from chondroitin sulfate proteoglycans (CSPG). Conclusions: In summary, our study suggested that miR-133b regulated neurite outgrowth via ERK1/2 and PI3K/Akt signaling pathway by RhoA suppression.

scholarly journals Purification and characterization of an 82-kD membrane protein as a neurite outgrowth factor binding protein: possible involvement of NOF binding protein in axonal outgrowth in developing retina.

1991 ◽  
Vol 112 (2) ◽  
pp. 313-322 ◽  
Author(s):  
H Taniura ◽  
C H Kuo ◽  
Y Hayashi ◽  
N Miki
Keyword(s):  
Membrane Protein ◽  
Neurite Outgrowth ◽  
Binding Protein ◽  
Axonal Outgrowth ◽  
Inhibition Assay ◽  
Retinal Neurons ◽  
Purification And Characterization ◽  
Sds Page ◽  
Doublet Band

Neurite outgrowth factor (NOF) is a glycoprotein isolated from an extract of gizzard that induces neurite outgrowth from cultured retinal or ciliary ganglionic (CG) neurons. We have reported that a glycoprotein of approximately 82 kD solubilized from gizzard muscles binds to NOF (ligand blotting) and inhibits the neurite promoting activity of NOF (inhibition assay). The 82-kD protein (NOF binding protein) was purified from gizzard muscle membranes as a doublet band on SDS-PAGE and a polyclonal antibody was raised against it. An NOF binding protein in developing retina exhibited the same physicochemical properties as that of the gizzard muscle. Quantitative decrease in NOF binding protein in embryonic retinas was observed after day 11 by the inhibition assay, ligand blotting, and immunoblotting, its decrease being parallel with reduction of NOF-induced neurite outgrowth of embryonic retinas. In an immunohistochemical study, the antibody stained only the optic fiber layers of the retinas of 8-d embryos, and this staining was no longer detectable in retinas of 18-d embryos. These results suggest that the 82-kD protein is a novel membrane protein that behaves as an NOF receptor and that the loss of neuritic response of the retinal neurons to NOF reflects a decrease in NOF receptor molecules.

scholarly journals Bioactivity-Guided Fractionation Identifies Amygdalin as a Potent Neurotrophic Agent from Herbal MedicineSemen PersicaeExtract

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Chuanbin Yang ◽  
Jia Zhao ◽  
Yuanyuan Cheng ◽  
Xuechen Li ◽  
Jianhui Rong
Keyword(s):  
Neurite Outgrowth ◽  
Pc12 Cells ◽  
Blood Stasis ◽  
Reverse Phase Hplc ◽  
Extracellular Signal Regulated Kinase ◽  
Neurotrophic Activity ◽  
Liquid Liquid Extraction ◽  
Fractionation Procedure ◽  
Extracellular Signal

Herbal medicineSemen Persicaeis widely used to treat blood stasis in Chinese medicine and other oriental folk medicines. Although little is known about the effects ofSemen Persicaeand its active compounds on neuron differentiation, our pilot study showed thatSemen Persicaeextract promoted neurite outgrowth in rat dopaminergic PC12 cells. In the present study, we developed a bioactivity-guided fractionation procedure for the characterization of the neurotrophic activity ofSemen Persicaeextract. The resultant fractions were assayed for neurite outgrowth in PC12 cells based on microscopic assessment. Through liquid-liquid extraction and reverse phase HPLC separation, a botanical glycoside amygdalin was isolated as the active compound responsible for the neurotrophic activity ofSemen Persicaeextract. Moreover, we found that amygdalin rapidly induced the activation of extracellular-signal-regulated kinase 1/2 (ERK1/2). A specific ERK1/2 inhibitor PD98059 attenuated the stimulatory effect of amygdalin on neurite outgrowth. Taken together, amygdalin was identified as a potent neurotrophic agent fromSemen Persicaeextract through a bioactivity-guided fractional procedure. The neurotrophic activity of amygdalin may be mediated by the activation of ERK1/2 pathway.

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