EvaGreen real-time PCR to determine horse meat adulteration in processed foods

LWT ◽  
2017 ◽  
Vol 75 ◽  
pp. 408-416 ◽  
Author(s):  
Liliana Meira ◽  
Joana Costa ◽  
Caterina Villa ◽  
Fernando Ramos ◽  
M. Beatriz P.P. Oliveira ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Processed Foods ◽  
Horse Meat

Detection of transgenic rice line TT51-1 in processed foods using conventional PCR, real-time PCR, and droplet digital PCR

Food Control ◽  
2019 ◽  
Vol 98 ◽  
pp. 380-388 ◽  
Author(s):  
Xiaofu Wang ◽  
Ting Tang ◽  
Qingmei Miao ◽  
Shilong Xie ◽  
Xiaoyun Chen ◽  
...  
Keyword(s):  
Real Time ◽  
Transgenic Rice ◽  
Real Time Pcr ◽  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Processed Foods ◽  
Conventional Pcr ◽  
Rice Line ◽  
Transgenic Rice Line

scholarly journals Highly Sensitive GMO Detection Using Real-Time PCR with a Large Amount of DNA Template: Single-Laboratory Validation

2018 ◽  
Vol 101 (2) ◽  
pp. 507-514 ◽  
Author(s):  
Junichi Mano ◽  
Shuko Hatano ◽  
Yasuaki Nagatomi ◽  
Satoshi Futo ◽  
Reona Takabatake ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Sensitive Detection ◽  
Detection Methods ◽  
35S Promoter ◽  
Processed Foods ◽  
Gmo Detection ◽  
Nos Terminator ◽  
Highly Sensitive ◽  
Dna Template

Abstract Current genetically modified organism (GMO) detection methods allow for sensitive detection. However, a further increase in sensitivity will enable more efficient testing for large grain samples and reliable testing for processed foods. In this study, we investigated real-time PCR-based GMO detection methods using a large amount of DNA template. We selected target sequences that are commonly introduced into many kinds of GM crops, i.e., 35S promoter and nopaline synthase (NOS) terminator. This makes the newly developed method applicable to a wide range of GMOs, including some unauthorized ones. The estimated LOD of the new method was 0.005% of GM maize events; to the best of our knowledge, this method is the most sensitive among the GM maize detection methods for which the LOD was evaluated in terms of GMO content. A 10-fold increase in the DNA amount as compared with the amount used under common testing conditions gave an approximately 10-fold reduction in the LOD without PCR inhibition. Our method is applicable to various analytical samples, including processed foods. The use of other primers and fluorescence probes would permit highly sensitive detection of various recombinant DNA sequences besides the 35S promoter and NOS terminator.

Detection of hazelnut (Corylus spp.) in processed foods using real-time PCR

Food Control ◽  
2007 ◽  
Vol 18 (2) ◽  
pp. 140-148 ◽  
Author(s):  
M. Arlorio ◽  
E. Cereti ◽  
J.D. Coïsson ◽  
F. Travaglia ◽  
A. Martelli
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Processed Foods

Real Time PCR to detect hazelnut allergen coding sequences in processed foods

2013 ◽  
Vol 138 (2-3) ◽  
pp. 1976-1981 ◽  
Author(s):  
Elisa Iniesto ◽  
Ana Jiménez ◽  
Nuria Prieto ◽  
Beatriz Cabanillas ◽  
Carmen Burbano ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Processed Foods ◽  
Coding Sequences

scholarly journals Improvements in Methods to Detect Wheat in Heat-processed Foods by Real-time PCR

2020 ◽  
Vol 26 (4) ◽  
pp. 517-526
Author(s):  
Etsuko Miyazaki ◽  
Megumi Kawasaki ◽  
Michihiko Miyamoto ◽  
Keiko Nakamuta ◽  
Takahisa Miyamoto
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Processed Foods

Quantification of DNA fragmentation in processed foods using real-time PCR

2017 ◽  
Vol 226 ◽  
pp. 149-155 ◽  
Author(s):  
Junichi Mano ◽  
Yasuyuki Nishitsuji ◽  
Yosuke Kikuchi ◽  
Shin-ichi Fukudome ◽  
Takuya Hayashida ◽  
...  
Keyword(s):  
Real Time ◽  
Dna Fragmentation ◽  
Real Time Pcr ◽  
Processed Foods

Application of real-time PCR for tree nut allergen detection in processed foods

2019 ◽  
Vol 60 (7) ◽  
pp. 1077-1093 ◽  
Author(s):  
Rosario Linacero ◽  
Africa Sanchiz ◽  
Isabel Ballesteros ◽  
Carmen Cuadrado
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Processed Foods ◽  
Tree Nut ◽  
Allergen Detection

Development of a real time PCR assay for detection of allergenic trace amounts of peanut (Arachis hypogaea) in processed foods

Food Control ◽  
2013 ◽  
Vol 30 (2) ◽  
pp. 480-490 ◽  
Author(s):  
Inés María López-Calleja ◽  
Silvia de la Cruz ◽  
Nicolette Pegels ◽  
Isabel González ◽  
Teresa García ◽  
...  
Keyword(s):  
Real Time ◽  
Arachis Hypogaea ◽  
Real Time Pcr ◽  
Processed Foods ◽  
Pcr Assay

Development of a real-time PCR approach for the relative quantitation of horse DNA

2015 ◽  
Vol 7 (20) ◽  
pp. 8590-8596 ◽  
Author(s):  
Gavin J. Nixon ◽  
Timothy M. Wilkes ◽  
Malcolm J. Burns
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Horse Meat ◽  
Relative Quantitation ◽  
Processing Steps

Figure illustrating the basic processing steps required to identify and quantify potential horse meat adulteration.

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