Interactions of heat shock protein 47 with collagen and the stress response: An unconventional chaperone model?

Life Sciences ◽  
2010 ◽  
Vol 87 (19-22) ◽  
pp. 579-586 ◽  
Author(s):  
J. Geraldine Sandana Mala ◽  
C. Rose
Keyword(s):  
Stress Response ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 47

Stress response of heavy metal mixture present in wastewater and leachate on heat-shock protein 47-transfected cells

2010 ◽  
Vol 29 (7) ◽  
pp. 1637-1647 ◽  
Author(s):  
Fahmi Ben Fredj ◽  
Mitsuteru Irie ◽  
Junkyu Han ◽  
Parida Yamada ◽  
Atef Limam ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Stress Response ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Metal Mixture ◽  
Heat Shock Protein 47 ◽  
Transfected Cells

Assessment of organic micropollutants occurrence in treated wastewater using heat shock protein 47 stress responses in Chinese hamster ovary cells and GC/MS-based non-target screening

2016 ◽  
Vol 74 (10) ◽  
pp. 2407-2416 ◽  
Author(s):  
Selma Etteieb ◽  
Atsushi Kawachi ◽  
Junkyu Han ◽  
Foued Elayni ◽  
Jamila Tarhouni ◽  
...  
Keyword(s):  
Stress Response ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Treated Wastewater ◽  
Organic Micropollutants ◽  
Heat Shock Protein 47 ◽  
Ovary Cells

Combining bioassays and analytical chemistry screening is a powerful approach to assess organic micropollutants which are the main contributors to toxic potential in complex mixtures of treated wastewater (TWW). The aim of this study was to perform a comprehensive toxicity assessment of treated effluents using stress response bioassays and then to assess the occurrence of the organic micropollutants which were responsible for this biological response using gas chromatography coupled with a mass spectrometry detector (GC/MS). Results showed that TWW samples induced significant stress response on Chinese hamster ovary cells, stably transfected with heat shock protein 47 promoter, at 0.1%, 1%, 5% and 10% concentrations. The organic chemical compounds responsible for stress response potential were identified at different percentage values using non-target chemical screening. Of the compounds detected in TWW1 and TWW4, 55.09% and 74.5% respectively, fell within the class of aliphatic hydrocarbons. Aliphatic hydrocarbons were also present in TWW3 at 26.46% whereas 11.96% corresponded to 6-acetyl-1,1,2,4,4,7-hexamethyltetralin and 16.08% to triethoxysilane. Moreover, 76.73% of TWW2 was recorded as decamethylcyclopentasiloxane (D5) and 17.44% as n-hexadecanoic acid.

Increased expression of collagen-binding heat shock protein 47 in murine bleomycin-induced pneumopathy

2003 ◽  
Vol 285 (4) ◽  
pp. L957-L963 ◽  
Author(s):  
Hiroshi Ishii ◽  
Hiroshi Mukae ◽  
Tomoyuki Kakugawa ◽  
Tetsuji Iwashita ◽  
Hideyuki Kaida ◽  
...  
Keyword(s):  
Heat Shock ◽  
Pulmonary Fibrosis ◽  
Heat Shock Protein ◽  
Smooth Muscle Actin ◽  
Protein A ◽  
Immunohistochemical Analysis ◽  
Rt Pcr ◽  
Heat Shock Protein 47 ◽  
Positive Type ◽  
Fibrotic Process

The 47-kDa heat shock protein 47 (HSP47) is a collagen-specific molecular chaperone that has been shown to play a major role during the processing and/or secretion of procollagen. Expression of HSP47 has been reported to increase in parallel with expression of collagens during the progression of various fibrosis models. The aim of the present study was to investigate the association between HSP47 expression and collagen accumulation in bleomycin (BLM)-induced murine fibrosis. We investigated the expression of HSP47 protein and mRNA using immunohistochemical analysis and semi-quantitative RT-PCR in murine BLM-induced pulmonary fibrosis. Immunohistochemical analysis showed that higher expression of HSP47 protein was present in BLM-induced pulmonary fibrosis compared with controls. HSP47 was localized predominantly in α-smooth muscle actin-positive myofibroblasts, F4/80 negative, surfactant protein-A-positive type II pneumocytes, and F4/80-positive macrophages. RT-PCR also demonstrated an increase of HSP47 mRNA expression in BLM-treated lungs. Moreover, the relative amounts of HSP47 mRNA correlated significantly with the lung hydroxyproline content as an indicator of pulmonary fibrosis in BLM-treated lungs ( r = 0.406, P <0.05). Our results suggest that these cells may play a role in the fibrotic process of BLM-treated lungs through upregulation of HSP47.

Heat Shock Protein 47 (HSP47)

2018 ◽  
pp. 1-7
Author(s):  
Fatin Hazwani Ibrahim ◽  
Normala Abd Latip ◽  
Mohd Firdaus Abdul-Wahab
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 47

Rapid Fibroblast Cell Culture Characterization with Impedimetric Label‐free Heat Shock Protein – 47 Biosensor

2020 ◽  
Vol 32 (10) ◽  
pp. 2310-2315
Author(s):  
Duygu Harmanci ◽  
Zihni Onur Uygun ◽  
Ayşe Koçak Sezgin ◽  
Cenk Demirdöver ◽  
Ferhan Girgin Sagin ◽  
...  
Keyword(s):  
Cell Culture ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Fibroblast Cell ◽  
Label Free ◽  
Heat Shock Protein 47 ◽  
Fibroblast Cell Culture

scholarly journals Effects of Ascorbic Acid and β-1,3-Glucan on Survival, Physiological Response and Flesh Quality of Cultured Tiger Grouper (Epinephelus fuscoguttatus) during Simulated Transport in Water

Biology ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 37 ◽  
Author(s):  
Bo Wu ◽  
Qi Wang ◽  
Jie Cao ◽  
Jun Mei ◽  
Jing Xie
Keyword(s):  
Ascorbic Acid ◽  
Survival Rate ◽  
Stress Response ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Flesh Quality ◽  
Epinephelus Fuscoguttatus ◽  
Tiger Grouper

Transport in water is the most common method for achieving high survival rates when transporting cultured fish in China; yet, transport success relies on proper water quality and conditions. This research was designed to explore the effects of ascorbic acid and β-1,3-glucan on survival, physiological responses, and flesh quality of farmed tiger grouper (Epinephelus fuscoguttatus) during simulated transport. The transport water temperature for live tiger grouper was 15 °C, which had the highest survival rate, the lowest stress response, and metabolic rate, and this will reduce the susceptibility to diseases. It is stated that β-1,3-glucan influences the changes of cortisol content, heat shock protein 70, IL-1β, and IgM transcription levels during simulated transport. Rather than using ascorbic acid alone (the A-group), β-1,3-glucan (3.2 mg/L) in the presence of ascorbic acid (25 mg/L) can effectively reduce the increase of transport-induced serum cortisol content, heat shock protein 70, and IL-1β, but stimulated IgM. 25 mg/L ascorbic acid and 3.2 mg/L β-1,3-glucan had no obvious effect on the nutritional indexes and flavor of live tiger grouper; however, these can effectively reduce the stress response, improve the innate immune activity, and ensure a higher survival rate.

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