scholarly journals Creation of an Artificial Artery Using Adipose-Derived Stem Cells (ASCs) and Small Intestine Submucosa (SIS)

2013 ◽  
Vol 58 (4) ◽  
pp. 1147-1148
Author(s):  
Kathleen M. Lamb ◽  
Aleksandra Policha ◽  
Lily Chang ◽  
Masaya Jimbo ◽  
Ping Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Small Intestine ◽  
Adipose Derived Stem Cells ◽  
Small Intestine Submucosa

Tracheal reconstruction by mesenchymal stem cells with small intestine submucosa in rabbits

2012 ◽  
Vol 76 (3) ◽  
pp. 345-351 ◽  
Author(s):  
Xiao Fei Du ◽  
Seong Keun Kwon ◽  
Jae-Jun Song ◽  
Chang Gun Cho ◽  
Seok-Won Park
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Small Intestine ◽  
Small Intestine Submucosa ◽  
Tracheal Reconstruction

scholarly journals A Preliminary Study of Constructing Tissue Engineered Oral Mucosa

2021 ◽  
Author(s):  
Qin Zhang ◽  
Yu-Jing Tian ◽  
Tao Ding ◽  
De-Hong Femg
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Small Intestine ◽  
Oral Mucosa ◽  
Porous Membrane ◽  
Adipose Derived Stem Cells ◽  
Oral Keratinocytes ◽  
Cell Scaffold ◽  
Compound Graft

Abstract Background: Adipose derived stem cells (ADSCs) have a great potential for tissue-engineering purposes, and they may be introduced in oral mucosa tissue engineering for urethroplasty. This study was aim to develop a tissue-engineered oral mucosa through seeding oral keratinocytes (OKs) and adipose derived stem cells (ADSCs) on small intestine submucosa (SIS). Methods: SIS was obtained from porcine small intestine, and OKs and ADSCs were obtained from canine sources and were cultured and expanded in vitro. The two cell lines were seeded on the two surfaces of the SIS, and the cell-scaffold compound graft was cultured in an air fluid level for 1 week.Results: The SIS exhibited a porous membrane, and no cells were found through HE staining. The model cultured with OK-SIS only formed a thin and loose epithelium. Whereas the model cultured with OK-SIS-ADSC was much thicker and denser. Conclusion: The co-cultured of ADSC and OK grew well on the SIS in which the OKs formed a multilayer of epithelium. So it is feasible to construct a tissue-engineered oral mucosa graft with ADSCs, OKs and SIS. The ADSCs contribute to a thicker epithelium formation.

scholarly journals A Preliminary Study of Constructing Tissue Engineered Oral Mucosa

2021 ◽  
Vol 7 (6) ◽  
Author(s):  
Zhang Q ◽  
Tian YJ ◽  
Ding T ◽  
Feng DH
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Small Intestine ◽  
Oral Mucosa ◽  
Porous Membrane ◽  
Adipose Derived Stem Cells ◽  
Oral Keratinocytes ◽  
Cell Scaffold ◽  
Compound Graft

Adipose derived stem cells (ADSCs) have a great potential for tissue-engineering purposes, and they may be introduced in oral mucosa tissue engineering for urethroplasty. This study was aimed to develop a tissue-engineered oral mucosa through seeding oral keratinocytes (OKs) and adipose derived stem cells (ADSCs) on small intestine submucosa (SIS). From August 2018 to October 2019, an observational study was conducted in the laboratory of our hospital, to develop a tissue-engineered oral mucosa.SIS was obtained from porcine small intestine, and OKs and ADSCs were obtained from canine sources and were cultured and expanded in vitro. The two cell lines were seeded on the two surfaces of the SIS, and the cell-scaffold compound graft was cultured in an air fluid level for 1 week. The SIS exhibited a porous membrane, and no cells were found through HE staining. The model cultured with OK-SIS only formed a thin and loose epithelium. Whereas the model cultured with OK-SIS-ADSC was much thicker and denser. The co-cultured of ADSC and OK grew well on the SIS in which the OKs formed a multilayer of epithelium. So it is feasible to construct a tissue-engineered oral mucosa graft with ADSCs, OKs and SIS. The ADSCs contribute to a thicker epithelium formation.

Small intestine submucosa and mesenchymal stem cells composite gel for scarless vocal fold regeneration

Biomaterials ◽  
2014 ◽  
Vol 35 (18) ◽  
pp. 4911-4918 ◽  
Author(s):  
Jae Won Choi ◽  
Ju Kyeong Park ◽  
Jae Won Chang ◽  
Da Yeon Kim ◽  
Moon Suk Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Small Intestine ◽  
Vocal Fold ◽  
Small Intestine Submucosa ◽  
Composite Gel

Small intestine submucosa sponge for in vivo support of tissue-engineered bone formation in the presence of rat bone marrow stem cells

Biomaterials ◽  
2010 ◽  
Vol 31 (6) ◽  
pp. 1104-1113 ◽  
Author(s):  
Kyung Sook Kim ◽  
Ju Young Lee ◽  
Yun Mi Kang ◽  
E.Sle Kim ◽  
Gyeong Hae Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Small Intestine ◽  
Bone Formation ◽  
Bone Marrow Stem Cells ◽  
Small Intestine Submucosa ◽  
Rat Bone

Comparison of Human Denuded Amniotic Membrane and Porcine Small Intestine Submucosa as Scaffolds for Limbal Mesenchymal Stem Cells

2018 ◽  
Vol 14 (5) ◽  
pp. 744-754 ◽  
Author(s):  
Liliana I. Sous Naasani ◽  
Cristiano Rodrigues ◽  
Jéssica Gonçalves Azevedo ◽  
Aline F. Damo Souza ◽  
Silvio Buchner ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Small Intestine ◽  
Amniotic Membrane ◽  
Small Intestine Submucosa ◽  
Porcine Small Intestine Submucosa

Adipose tissue‐derived stem cells upon decellularized ovine small intestine submucosa for tissue regeneration: An optimization and comparison method

2019 ◽  
Vol 235 (2) ◽  
pp. 1556-1567
Author(s):  
Ahad Ferdowsi Khosroshahi ◽  
Jafar Soleimani Rad ◽  
Razie Kheirjou ◽  
Babak Roshangar ◽  
Morteza Rashtbar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Small Intestine ◽  
Tissue Regeneration ◽  
Comparison Method ◽  
Small Intestine Submucosa
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