scholarly journals Predifferentiated Smooth Muscle-Like Adipose-Derived Stem Cells for Bladder Engineering

2020 ◽  
Vol 26 (17-18) ◽  
pp. 979-992
Author(s):  
Jakub Smolar ◽  
Maya Horst ◽  
Souzan Salemi ◽  
Daniel Eberli
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Adipose Derived Stem Cells

scholarly journals MicroRNA-145 regulates the differentiation of human adipose-derived stem cells to smooth muscle cells via targeting Krüppel-like factor 4

2017 ◽  
Vol 15 (6) ◽  
pp. 3787-3795 ◽  
Author(s):  
Kaisaier Aji ◽  
Yun Zhang ◽  
Abudusaimi Aimaiti ◽  
Yujie Wang ◽  
Mulati Rexiati ◽  
...  
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Adipose Derived Stem Cells

scholarly journals Transplantation of adipose derived stem cells overexpressing inducible nitric oxide synthase ameliorates diabetes mellitus induced erectile dysfunction in rats

2019 ◽  
Author(s):  
Yan Zhang ◽  
Jun Yang ◽  
Li Zhuan ◽  
Guanghui Zang ◽  
Tao Wang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Diabetes Mellitus ◽  
Stem Cells ◽  
Smooth Muscle ◽  
Erectile Dysfunction ◽  
Inducible Nitric Oxide Synthase ◽  
Collagen Iv ◽  
Adipose Derived Stem Cells ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

Background: Erectile dysfunction is a major complication of diabetes mellitus. Adipose derived stem cells (ADSCs) has attracted much attention as a promising tool for the treatment of diabetes mellitus induced erectile dysfunction (DMED). Inducible nitric oxide synthase (iNOS) plays an important role in protecting penile tissues from fibrosis. The aim of this study was to determine the efficacy of ADSCs overexpressing iNOS on DMED in rats. Methods: ADSCs were isolated and infected with adenovirus overexpressing iNOS (named as ADSCs-iNOS). The expression of iNOS was detected using western blot analysis and real-time PCR. Rats were randomly assigned into five groups: control group, DMED group, ADSCs group, ADSCs-EGFP group and ADSCs-iNOS group. 5×105 cells were given once via the intracorporal route. Two weeks after treatment, erectile function was assessed by electrical stimulation of the cavernous nerve. Penile tissues were obtained and evaluated at histology level. Results: We found that ADSCs-iNOS had significantly higher expression of iNOS at mRNA and protein levels and generated more nitric oxide. ADSCs-iNOS reduced collagen I and collagen IV expression of corpus cavernosum smooth muscle cells (CCSMCs) in cell co-culture model. Transforming growth factor-β1 expression and p-Smad2/3 to Smad2/3 ratio in CCSMCs reduced following co-culture with ADSCs-iNOS. Injection of ADSCs-iNOS significantly ameliorated DMED in rats and decreased collagen/smooth muscle cell ratio of penile tissues. Moreover, elevated nitric oxide and cGMP concentrations were detected in penile tissues of ADSCs-iNOS group. Conclusion: Taken together, ADSCs-iNOS significantly improve erectile function of DMED rats. The therapeutic effect may be achieved by increased nitric oxide generation and the suppression of collagen I and collagen IV expression in the CCSMCs to decrease penile fibrosis.

scholarly journals TGF-β1 Regulates Smooth Muscle Cells Differentiation from Adipose-Derived Stem Cells via the Wnt/β-Catenin Pathway

2021 ◽  
Author(s):  
Xuling Lv ◽  
Hao Chen ◽  
Zikai Zhang ◽  
Tian Li ◽  
Qing Wei ◽  
...  
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Pelvic Floor ◽  
Signaling Pathway ◽  
Myogenic Differentiation ◽  
Pelvic Floor Dysfunction ◽  
Muscle Cells ◽  
Adipose Derived Stem Cells ◽  
Tgf Β1

Abstract Background: Pelvic floor dysfunction (PFD) is a spectrum of disorders including stress urinary incontinence and pelvic organ prolapse. Transforming growth factor-β1 (TGF-β1) can induce mesenchymal stem cells (MSCs) to differentiate into smooth muscle cells (SMCs). SMCs derived from adipose-derived stem cells (ADSCs) can be used to repair damaged pelvic floor smooth muscle tissues, which is of great interest for clinical applications using stem cell therapy for PFD. The Wnt/β-catenin pathway acts as a decisive factor in the fate of stem cells.Methods and Results: In this study, we used medium containing TGF-β1, TGF-β1 inhibitor LY2109761, or Wnt/β-catenin inhibitor KYA1797K, to induce ADCSs to differentiate into SMCs in vitro to explore the influence of TGF-β1 on the myogenic differentiation of ADCSs via the Wnt/β-catenin pathway. Results: 1) TGF-β1 induces ADSC-derived SMCs to hyper-express the SMC markers including SMA-α, Desmin, Calponin, and SMMHC ; 2) TGF-β1 activates the Wnt/β-catenin signaling pathway in ADSCs. After blocking TGF-β1, the Wnt/β-catenin pathway and myogenic differentiation in cells were inhibited; 3) the Wnt/β-catenin pathway is involved in the differentiation of ADSCs into SMCs. After differentiation induction, the synchronized changes in the activation of Wnt/β-catenin signaling and the expression of SMC-specific proteins showed a trend of simultaneous changes, and after the inhibition of the Wnt pathway, the adult muscle differentiation was significantly inhibited.Conclusions: We established a simpler and more efficient method for inducing ADSCs to differentiate into SMCs using TGF-β1 and demonstrated that the Wnt/β-catenin signaling pathway is activated during this process.

Autophagy is needed during the differentiation of adipose derived stem cells to functional smooth muscle cells for use in bladder engineering

2019 ◽  
Vol 18 (1) ◽  
pp. e1266
Author(s):  
S. Salemi ◽  
D. Haralampieva-Mohr ◽  
B. Kranzbühler ◽  
A. Mortezavi ◽  
T. Sulser ◽  
...  
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Adipose Derived Stem Cells

Cathelicidin LL37 promotes epithelial and smooth-muscle-like differentiation of adipose-derived stem cells through the Wnt/β-catenin and NF-κB pathways

2017 ◽  
Vol 82 (11) ◽  
pp. 1336-1345 ◽  
Author(s):  
Yongwei Li ◽  
Zhengfei Shan ◽  
Bin Yang ◽  
Diandong Yang ◽  
Changping Men ◽  
...  
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Adipose Derived Stem Cells

scholarly journals Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγ

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Kaisaier Aji ◽  
Munila Maimaijiang ◽  
Abudusaimi Aimaiti ◽  
Mulati Rexiati ◽  
Baihetiya Azhati ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Smooth Muscle ◽  
Protein Kinase ◽  
Adipose Derived Stem Cells ◽  
Dependent Protein Kinase ◽  
Protein Levels ◽  
Adult Mesenchymal Stem Cells ◽  
Dependent Protein ◽  
Muscle Contractile

The multifunctional Ca2+/calmodulin-dependent protein kinase II (CaMKII) is known to participate in maintenance and switches of smooth muscle cell (SMC) phenotypes. However, which isoform of CaMKII is involved in differentiation of adult mesenchymal stem cells into contractile SMCs remains unclear. In the present study, we detectedγisoform of CaMKII in differentiation of human adipose derived stem cells (hASCs) into SMCs that resulted from treatment with TGF-β1 and BMP4 in combination for 7 days. The results showed that CaMKIIγincreased gradually during differentiation of hASCs as determined by real-time PCR and western blot analysis. The siRNA-mediated knockdown of CaMKIIγdecreased the protein levels and transcriptional levels of smooth muscle contractile markers (a-SMA, SM22a, calponin, and SM-MHC), while CaMKIIγoverexpression increases the transcriptional and protein levels of smooth muscle contractile markers. These results suggested thatγisoform of CaMKII plays a significant role in smooth muscle differentiation of hASCs.

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