Upregulation of VEGF in Adult Leptin Deficient Diabetic Mouse Adipose Derived Stromal Cells Using Biodegradable Nanoparticulate Polymeric Vectors Can Be Used to Accelerate Wound Closure in a Diabetic Mouse Excisional Wound Healing Model

2012 ◽  
Vol 172 (2) ◽  
pp. 205
Author(s):  
A.C. Nauta ◽  
L. Deveza ◽  
D. Montoro ◽  
J. Hyun ◽  
G.C. Gurtner ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stromal Cells ◽  
Wound Closure ◽  
Diabetic Mouse ◽  
Adipose Derived Stromal Cells ◽  
Excisional Wound ◽  
Wound Healing Model ◽  
Polymeric Vectors ◽  
Healing Model

scholarly journals Single-Cell Gene Expression Analysis and Evaluation of the Therapeutic Function of Murine Adipose-Derived Stromal Cells (ASCs) from the Subcutaneous and Visceral Compartment

2018 ◽  
Vol 2018 ◽  
pp. 1-9
Author(s):  
Dominik Pförringer ◽  
Matthias M. Aitzetmüller ◽  
Elizabeth A. Brett ◽  
Khosrow S. Houschyar ◽  
Richard Schäfer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Wound Healing ◽  
Single Cell ◽  
Stromal Cells ◽  
Single Cell Level ◽  
Cell Level ◽  
Adipose Derived Stromal Cells ◽  
Wound Healing Model ◽  
Healing Model

Introduction. Adipose-derived stromal cells (ASCs) are a promising resource for wound healing and tissue regeneration because of their multipotent properties and cytokine secretion. ASCs are typically isolated from the subcutaneous fat compartment, but can also be obtained from visceral adipose tissue. The data on their equivalence diverges. The present study analyzes the cell-specific gene expression profiles and functional differences of ASCs derived from the subcutaneous (S-ASCs) and the visceral (V-ASCs) compartment. Material and Methods. Subcutaneous and visceral ASCs were obtained from mouse inguinal fat and omentum. The transcriptional profiles of the ASCs were compared on single-cell level. S-ASCs and V-ASCs were then compared in a murine wound healing model to evaluate their regenerative functionality. Results. On a single-cell level, S-ASCs and V-ASCs displayed distinct transcriptional profiles. Specifically, significant differences were detected in genes associated with neoangiogenesis and tissue remodeling (for example, Ccl2, Hif1α, Fgf7, and Igf). In addition, a different subpopulation ecology could be identified employing a cluster model. Nevertheless, both S-ASCs and V-ASCs induced accelerated healing rates and neoangiogenesis in a mouse wound healing model. Conclusion. With similar therapeutic potential in vivo, the significantly different gene expression patterns of ASCs from the subcutaneous and visceral compartments suggest different signaling pathways underlying their efficacy. This study clearly demonstrates that review of transcriptional results in vivo is advisable to confirm the tentative effect of cell therapies.

scholarly journals Morphological Evaluation of Wound Healing Events in the Excisional Wound Healing Model in Rats

BIO-PROTOCOL ◽  
2019 ◽  
Vol 9 (13) ◽  
Author(s):  
Ligia Estevão ◽  
Puebla Cassini-Vieira ◽  
Ana Greice Leite ◽  
Apolônia Bulhões ◽  
Lucíola da Barcelos ◽  
...  
Keyword(s):  
Wound Healing ◽  
Morphological Evaluation ◽  
Excisional Wound ◽  
Wound Healing Model ◽  
Healing Model

Polyethylene Glycol–Based Nanocerium Improves Healing Responses in Excisional and Incisional Wound Models in Rats

2020 ◽  
pp. 153473462091210
Author(s):  
Tara Kardan ◽  
Rahim Mohammadi ◽  
Saeed Taghavifar ◽  
Marzieh Cheraghi ◽  
Ashkan Yahoo ◽  
...  
Keyword(s):  
Wound Healing ◽  
Polyethylene Glycol ◽  
Granulation Tissue ◽  
Wistar Rats ◽  
Wound Area ◽  
Therapeutic Benefits ◽  
Significant Difference ◽  
Excisional Wound ◽  
Wound Healing Model ◽  
Healing Model

Applications of nanotechnology have gained progressive interest for regeneration of injured wound tissue. The aim of the present study was to evaluate effects of polyethylene glycol (PEG)-based nanocerium on excisional and incisional wound models in rats. For excisional wound healing model, 24 male white Wistar rats were randomized into 4 groups of 6 rats each: control group with creation of wounds and no treatment, PEG group with creation of wounds and dressing the wound with PEG, NanoCer group with application of 1 mL nanocerium on the wound, and PEG/NanoCer group with dressing the wound with PEG-based nanocerium. Wound size was measured on days 6, 9, 12, 15, 18, and 21 postsurgery. For incisional wound healing model, 24 healthy male Wistar rats were randomized into 4 groups of 6 rats each the same way in the excisional wound model. Reduction in wound area, hydroxyproline contents, and biomechanical parameters indicated that there was a significant difference ( P > .05) between PEG/NanoCer and other groups. Biomechanical testing was performed on day 9 postsurgery in the incisional model. Biochemical and quantitative histological studies demonstrated that there was a significant difference ( P > .05) between PEG/NanoCer and other groups. PEG/NanoCer offered potential advantages in wound healing acceleration and improvement through angiogenesis stimulation, fibroblast proliferation, and granulation tissue formation on early days of healing phases. Acceleration in wound repair was associated with earlier wound area reduction and enhanced tensile strength of damaged area by rearrangement of granulation tissue and collagen fibers. PEG-based nanocerium could have therapeutic benefits in wound healing.

Thrombin as important factor for cutaneous wound healing: Comparison of fibrin biomatrices in vitro and in a rat excisional wound healing model

2014 ◽  
Vol 22 (6) ◽  
pp. 740-748 ◽  
Author(s):  
Alfred Gugerell ◽  
Waltraud Pasteiner ◽  
Sylvia Nürnberger ◽  
Johanna Kober ◽  
Alexandra Meinl ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound ◽  
Excisional Wound ◽  
Wound Healing Model ◽  
Healing Model

scholarly journals Adipose-derived Stromal Cells Overexpressing Vascular Endothelial Growth Factor Accelerate Mouse Excisional Wound Healing

2013 ◽  
Vol 21 (2) ◽  
pp. 445-455 ◽  
Author(s):  
Allison Nauta ◽  
Catharina Seidel ◽  
Lorenzo Deveza ◽  
Daniel Montoro ◽  
Monica Grova ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Wound Healing ◽  
Growth Factor ◽  
Stromal Cells ◽  
Vascular Endothelial ◽  
Adipose Derived Stromal Cells ◽  
Excisional Wound ◽  
Endothelial Growth Factor

Assessment of full-thickness wounds in the genetically diabetic mouse for suitability as a wound healing model

1995 ◽  
Vol 3 (3) ◽  
pp. 351-358 ◽  
Author(s):  
Lucy H. Senter ◽  
Edmund K. Legrand ◽  
Karen E. Laemmerhirt ◽  
Timothy C. Kiorpes
Keyword(s):  
Wound Healing ◽  
Diabetic Mouse ◽  
Full Thickness ◽  
Wound Healing Model ◽  
Healing Model

scholarly journals Galvanic microparticles increase migration of human dermal fibroblasts in a wound-healing model via reactive oxygen species pathway

2014 ◽  
Vol 320 (1) ◽  
pp. 79-91 ◽  
Author(s):  
Nina Tandon ◽  
Elisa Cimetta ◽  
Aranzazu Villasante ◽  
Nicolette Kupferstein ◽  
Michael D. Southall ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Wound Healing ◽  
Reactive Oxygen ◽  
Dermal Fibroblasts ◽  
Oxygen Species ◽  
Human Dermal Fibroblasts ◽  
Wound Healing Model ◽  
Healing Model

scholarly journals Transcriptomic Analysis of a Diabetic Skin-Humanized Mouse Model Dissects Molecular Pathways Underlying the Delayed Wound Healing Response

Genes ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 47
Author(s):  
Carlos León ◽  
Francisco García-García ◽  
Sara Llames ◽  
Eva García-Pérez ◽  
Marta Carretero ◽  
...  
Keyword(s):  
Wound Healing ◽  
Functional Enrichment ◽  
Preclinical Model ◽  
Published Data ◽  
Diabetic Patients ◽  
Ulcer Formation ◽  
Histological Features ◽  
Depth Analysis ◽  
Wound Healing Model ◽  
Healing Model

Defective healing leading to cutaneous ulcer formation is one of the most feared complications of diabetes due to its consequences on patients’ quality of life and on the healthcare system. A more in-depth analysis of the underlying molecular pathophysiology is required to develop effective healing-promoting therapies for those patients. Major architectural and functional differences with human epidermis limit extrapolation of results coming from rodents and other small mammal-healing models. Therefore, the search for reliable humanized models has become mandatory. Previously, we developed a diabetes-induced delayed humanized wound healing model that faithfully recapitulated the major histological features of such skin repair-deficient condition. Herein, we present the results of a transcriptomic and functional enrichment analysis followed by a mechanistic analysis performed in such humanized wound healing model. The deregulation of genes implicated in functions such as angiogenesis, apoptosis, and inflammatory signaling processes were evidenced, confirming published data in diabetic patients that in fact might also underlie some of the histological features previously reported in the delayed skin-humanized healing model. Altogether, these molecular findings support the utility of such preclinical model as a valuable tool to gain insight into the molecular basis of the delayed diabetic healing with potential impact in the translational medicine field.

Sanativo Wound Healing Product Does Not Accelerate Reepithelialization in a Mouse Cutaneous Wound Healing Model

2017 ◽  
Vol 139 (2) ◽  
pp. 343-352 ◽  
Author(s):  
Clement D. Marshall ◽  
Michael S. Hu ◽  
Tripp Leavitt ◽  
Leandra A. Barnes ◽  
Alexander T. M. Cheung ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound ◽  
Wound Healing Model ◽  
Healing Model
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