Thrombin as important factor for cutaneous wound healing: Comparison of fibrin biomatrices in vitro and in a rat excisional wound healing model

2014 ◽  
Vol 22 (6) ◽  
pp. 740-748 ◽  
Author(s):  
Alfred Gugerell ◽  
Waltraud Pasteiner ◽  
Sylvia Nürnberger ◽  
Johanna Kober ◽  
Alexandra Meinl ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound ◽  
Excisional Wound ◽  
Wound Healing Model ◽  
Healing Model

Sanativo Wound Healing Product Does Not Accelerate Reepithelialization in a Mouse Cutaneous Wound Healing Model

2017 ◽  
Vol 139 (2) ◽  
pp. 343-352 ◽  
Author(s):  
Clement D. Marshall ◽  
Michael S. Hu ◽  
Tripp Leavitt ◽  
Leandra A. Barnes ◽  
Alexander T. M. Cheung ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound ◽  
Wound Healing Model ◽  
Healing Model

scholarly journals RNAseq Analysis of Treatment-dependent Signaling Changes During Inflammation in a Mouse Cutaneous Wound Healing Model.

2021 ◽  
Author(s):  
III Georges St. Laurent ◽  
Ian Toma ◽  
Bernd Seilheimer ◽  
Konstantin Cesnulevicius ◽  
Myron Schultz ◽  
...  
Keyword(s):  
Wound Healing ◽  
Placebo Control ◽  
Therapeutic Effects ◽  
Mrna Levels ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound ◽  
Synthetic Pathway ◽  
Cox Inhibitor ◽  
Wound Healing Model ◽  
Healing Model

Abstract Background: Despite proven therapeutic effects in inflammatory conditions, the specific mechanisms of phytochemical therapies are not well understood. The transcriptome effects of Tr14 (Traumeel), a multicomponent natural product, and diclofenac, a non-selective cyclooxygenase (COX) inhibitor, were compared in a mouse cutaneous wound healing model to identify both known and novel pathways for the anti-inflammatory effect of plant-derived natural products. Methods: Skin samples from abraded mice were analyzed by single-molecule, amplification-free RNAseq transcript profiling at 7 points between 12-192 hours after injury. Immediately after injury, the wounds were treated with either diclofenac, Tr14, or placebo control (n=7 per group/time). RNAseq levels were compared between treatment and control at each time point using a systems biology approach. Results: At early time points (12-36 hours), both control and Tr14-treated wounds showed marked increase in the inducible COX2 enzyme mRNA, while diclofenac-treated wounds did not. Tr14, in contrast, modulated lipoxygenase transcripts, especially ALOX12/15, and phospholipases involved in arachidonate metabolism. Notably, Tr14 modulated a group of cell-type specific markers, including the T cell receptor, that could be explained by an overarching effect on the type of cells that were recruited into the wound tissue. Conclusions: Tr14 and diclofenac had very different effects on the COX/LOX synthetic pathway after cutaneous wounding. Tr14 allowed normal autoinduction of COX2 mRNA, but suppressed mRNA levels for key enzymes in the leukotriene synthetic pathway. Tr14 appeared to have a broad ‘phytocellular’ effect on the wound transcriptome by altering the balance of cell types present in the wound.

scholarly journals RNAseq analysis of treatment-dependent signaling changes during inflammation in a mouse cutaneous wound healing model

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Georges St. Laurent ◽  
Ian Toma ◽  
Bernd Seilheimer ◽  
Konstantin Cesnulevicius ◽  
Myron Schultz ◽  
...  
Keyword(s):  
Wound Healing ◽  
Placebo Control ◽  
Therapeutic Effects ◽  
Mrna Levels ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound ◽  
Synthetic Pathway ◽  
Cox Inhibitor ◽  
Wound Healing Model ◽  
Healing Model

Abstract Background Despite proven therapeutic effects in inflammatory conditions, the specific mechanisms of phytochemical therapies are not well understood. The transcriptome effects of Traumeel (Tr14), a multicomponent natural product, and diclofenac, a non-selective cyclooxygenase (COX) inhibitor, were compared in a mouse cutaneous wound healing model to identify both known and novel pathways for the anti-inflammatory effect of plant-derived natural products. Methods Skin samples from abraded mice were analyzed by single-molecule, amplification-free RNAseq transcript profiling at 7 points between 12 and 192 h after injury. Immediately after injury, the wounds were treated with either diclofenac, Tr14, or placebo control (n = 7 per group/time). RNAseq levels were compared between treatment and control at each time point using a systems biology approach. Results At early time points (12–36 h), both control and Tr14-treated wounds showed marked increase in the inducible COX2 enzyme mRNA, while diclofenac-treated wounds did not. Tr14, in contrast, modulated lipoxygenase transcripts, especially ALOX12/15, and phospholipases involved in arachidonate metabolism. Notably, Tr14 modulated a group of cell-type specific markers, including the T cell receptor, that could be explained by an overarching effect on the type of cells that were recruited into the wound tissue. Conclusions Tr14 and diclofenac had very different effects on the COX/LOX synthetic pathway after cutaneous wounding. Tr14 allowed normal autoinduction of COX2 mRNA, but suppressed mRNA levels for key enzymes in the leukotriene synthetic pathway. Tr14 appeared to have a broad ‘phytocellular’ effect on the wound transcriptome by altering the balance of cell types present in the wound.

scholarly journals Morphological Evaluation of Wound Healing Events in the Excisional Wound Healing Model in Rats

BIO-PROTOCOL ◽  
2019 ◽  
Vol 9 (13) ◽  
Author(s):  
Ligia Estevão ◽  
Puebla Cassini-Vieira ◽  
Ana Greice Leite ◽  
Apolônia Bulhões ◽  
Lucíola da Barcelos ◽  
...  
Keyword(s):  
Wound Healing ◽  
Morphological Evaluation ◽  
Excisional Wound ◽  
Wound Healing Model ◽  
Healing Model

scholarly journals Microfluidic and Lab-on-a-Chip Systems for Cutaneous Wound Healing Studies

2021 ◽  
Author(s):  
Ghazal Shabestani Monfared ◽  
Peter Ertl ◽  
Mario Rothbauer
Keyword(s):  
Wound Healing ◽  
Molecular Mechanisms ◽  
Chronic Wounds ◽  
Cell Communication ◽  
Lab On A Chip ◽  
Tissue Level ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound

Cutaneous wound healing is a complex multi-stage process involving direct and indirect cell communication events with the aim of efficiently restoring the barrier function of the skin. One key aspect in cutaneous wound healing is associated with cell movement and migration into the physically, chemically and biologically injured area resulting in wound closure. Understanding the conditions under which cell migration is impaired and elucidating the cellular and molecular mechanisms that improve healing dynamics is therefore crucial in devising novel therapeutic strategies to elevate patient suffering, reduce scaring and eliminate chronic wounds. Following the global trend towards automation, miniaturization and integration of cell-based assays into microphysiological systems, conventional wound healing assays such as the scratch assay or cell exclusion assay have recently been translated and improved using microfluidics and lab-on-a-chip technologies. These miniaturized cell analysis systems allow precise spatial and temporal control over a range of dynamic microenvironmental factors including shear stress, biochemical and oxygen gradients to create more reliable in vitro models that resemble the in vivo microenvironment of a wound more closely on a molecular, cellular, and tissue level. The current review provides (a) an overview on the main molecular and cellular processes that take place during wound healing, (b) a brief introduction into conventional in vitro wound healing assays, and (c) a perspective on future cutaneous and vascular wound healing research using microfluidic technology.

Polyethylene Glycol–Based Nanocerium Improves Healing Responses in Excisional and Incisional Wound Models in Rats

2020 ◽  
pp. 153473462091210
Author(s):  
Tara Kardan ◽  
Rahim Mohammadi ◽  
Saeed Taghavifar ◽  
Marzieh Cheraghi ◽  
Ashkan Yahoo ◽  
...  
Keyword(s):  
Wound Healing ◽  
Polyethylene Glycol ◽  
Granulation Tissue ◽  
Wistar Rats ◽  
Wound Area ◽  
Therapeutic Benefits ◽  
Significant Difference ◽  
Excisional Wound ◽  
Wound Healing Model ◽  
Healing Model

Applications of nanotechnology have gained progressive interest for regeneration of injured wound tissue. The aim of the present study was to evaluate effects of polyethylene glycol (PEG)-based nanocerium on excisional and incisional wound models in rats. For excisional wound healing model, 24 male white Wistar rats were randomized into 4 groups of 6 rats each: control group with creation of wounds and no treatment, PEG group with creation of wounds and dressing the wound with PEG, NanoCer group with application of 1 mL nanocerium on the wound, and PEG/NanoCer group with dressing the wound with PEG-based nanocerium. Wound size was measured on days 6, 9, 12, 15, 18, and 21 postsurgery. For incisional wound healing model, 24 healthy male Wistar rats were randomized into 4 groups of 6 rats each the same way in the excisional wound model. Reduction in wound area, hydroxyproline contents, and biomechanical parameters indicated that there was a significant difference ( P > .05) between PEG/NanoCer and other groups. Biomechanical testing was performed on day 9 postsurgery in the incisional model. Biochemical and quantitative histological studies demonstrated that there was a significant difference ( P > .05) between PEG/NanoCer and other groups. PEG/NanoCer offered potential advantages in wound healing acceleration and improvement through angiogenesis stimulation, fibroblast proliferation, and granulation tissue formation on early days of healing phases. Acceleration in wound repair was associated with earlier wound area reduction and enhanced tensile strength of damaged area by rearrangement of granulation tissue and collagen fibers. PEG-based nanocerium could have therapeutic benefits in wound healing.

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