Structure of the DNA-binding domain of human myelin-gene regulatory factor reveals its potential protein-DNA recognition mode

2018 ◽  
Vol 203 (2) ◽  
pp. 170-178 ◽  
Author(s):  
Baohua Chen ◽  
Yun Zhu ◽  
Sheng Ye ◽  
Rongguang Zhang
Keyword(s):  
Dna Binding ◽  
Dna Recognition ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Regulatory Factor ◽  
Gene Regulatory ◽  
Myelin Gene

scholarly journals DNA-binding domain of myelin-gene regulatory factor: purification, crystallization and X-ray analysis. Corrigendum

2017 ◽  
Vol 73 (12) ◽  
pp. 713-713
Author(s):  
WenYu Wu ◽  
Xiangkai Zhen ◽  
Ning Shi
Keyword(s):  
Dna Binding ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Regulatory Factor ◽  
Acta Cryst ◽  
X Ray ◽  
Gene Regulatory ◽  
Myelin Gene

An extra affiliation is added for the authors of the article by Wu et al. [(2017), Acta Cryst. F73, 393–397].

scholarly journals Crystal structure of the DNA-binding domain of Myelin-gene Regulatory Factor

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Xiangkai Zhen ◽  
Bowen Li ◽  
Fen Hu ◽  
Shufeng Yan ◽  
Gabriele Meloni ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Dna Binding ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Regulatory Factor ◽  
Gene Regulatory ◽  
Myelin Gene

scholarly journals DNA-binding domain of myelin-gene regulatory factor: purification, crystallization and X-ray analysis

2017 ◽  
Vol 73 (7) ◽  
pp. 393-397
Author(s):  
WenYu Wu ◽  
Xiangkai Zhen ◽  
Ning Shi
Keyword(s):  
Transcription Factor ◽  
Dna Binding ◽  
Myelin Sheath ◽  
Binding Domain ◽  
Demyelinating Diseases ◽  
Endoplasmic Reticulum Membrane ◽  
Dna Binding Domain ◽  
Regulatory Factor ◽  
Gene Regulatory ◽  
Myelin Gene

The myelin sheath, which envelops axons in the vertebrate central nervous system, is crucial for the rapid conduction of action potentials. Myelin-gene regulatory factor (MRF) is a recently identified transcription factor that is required for myelin-sheath formation. Loss of MRF leads to demyelinating diseases and motor learning deficiency. MRF is a membrane-bound transcription factor that undergoes autocleavage from the endoplasmic reticulum membrane. The N-terminus of MRF contains a DNA-binding domain (DBD) that functions as a homotrimer. In this study, the MRF DBD was cloned, purified and crystallized in order to understand the molecular mechanism that regulates the transcription of myelin genes. Selenomethionine was subsequently introduced into the crystals to obtain the phases for the MRF DBD structure. The native and selenomethionine-labelled crystals exhibited diffraction to 2.50 and 2.51 Å resolution, respectively. The crystals belonged to space groupP321 and the selenomethionine-labelled crystals had unit-cell parametersa= 104.0,b= 104.0,c= 46.7 Å, α = 90, β = 90, γ = 120°. The calculated Matthews coefficient was 3.04 Å3Da−1and the solvent content was 59.5%, indicating the presence of one MRF DBD molecule in the asymmetric unit.

scholarly journals Secondary structure and folding topology of the DNA binding domain of interferon regulatory factor 2, as revealed by NMR spectroscopy

FEBS Letters ◽  
1995 ◽  
Vol 359 (2-3) ◽  
pp. 184-188 ◽  
Author(s):  
Koichi Uegaki ◽  
Masahiro Shirakawa ◽  
Hisashi Harada ◽  
Tadatsugu Taniguchi ◽  
Yoshimasa Kyogoku
Keyword(s):  
Nmr Spectroscopy ◽  
Secondary Structure ◽  
Dna Binding ◽  
Interferon Regulatory Factor ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Regulatory Factor

scholarly journals An IFN regulatory factor-2 DNA-binding domain dominant negative mutant exhibits altered cell growth and gene expression

Oncogene ◽  
2000 ◽  
Vol 19 (11) ◽  
pp. 1411-1418 ◽  
Author(s):  
Y R Rubinstein ◽  
P H Driggers ◽  
V V Ogryzko ◽  
A M Thornton ◽  
K Ozato ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Growth ◽  
Dna Binding ◽  
Binding Domain ◽  
Dominant Negative ◽  
Dominant Negative Mutant ◽  
Dna Binding Domain ◽  
Regulatory Factor ◽  
Altered Cell ◽  
Negative Mutant

scholarly journals Unique Structure of the DNA Binding Domain of Interferon Regulatory Factor 2 Determined by NMR Spectroscopy.

1994 ◽  
Vol 70 (10) ◽  
pp. 200-204
Author(s):  
Koichi UEGAKI ◽  
Masahiro SHIRAKAWA ◽  
Hisashi HARADA ◽  
Tadatsugu TANIGUCHI ◽  
Yoshimasa KYOGOKU
Keyword(s):  
Nmr Spectroscopy ◽  
Dna Binding ◽  
Interferon Regulatory Factor ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Regulatory Factor ◽  
Unique Structure

The cavity in the hydrophobic core of Myb DNA-binding domain is reserved for DNA recognition and trans-activation

1996 ◽  
Vol 3 (2) ◽  
pp. 178-187 ◽  
Author(s):  
Kazuhiro Ogata ◽  
Chie Kanei-Ishii ◽  
Motoko Sasaki ◽  
Hideki Hatanaka ◽  
Aritaka Nagadoi ◽  
...  
Keyword(s):  
Dna Binding ◽  
Dna Recognition ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Hydrophobic Core
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