scholarly journals 3D Imaging of mammalian cells with ion-abrasion scanning electron microscopy

2009 ◽  
Vol 166 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Jurgen A.W. Heymann ◽  
Dan Shi ◽  
Sang Kim ◽  
Donald Bliss ◽  
Jacqueline L.S. Milne ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Mammalian Cells ◽  
3D Imaging ◽  
Scanning Electron

scholarly journals 3D imaging by serial block face scanning electron microscopy for materials science using ultramicrotomy

2016 ◽  
Vol 163 ◽  
pp. 6-18 ◽  
Author(s):  
Teruo Hashimoto ◽  
George E. Thompson ◽  
Xiaorong Zhou ◽  
Philip J. Withers
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
3D Imaging ◽  
Materials Science ◽  
Face Scanning ◽  
Block Face ◽  
Scanning Electron

Arg-substituted VmCT1 analogs reveals promising candidate for the development of new antichagasic agent

Parasitology ◽  
2020 ◽  
Vol 147 (14) ◽  
pp. 1810-1818
Author(s):  
Cibele Nicolaski Pedron ◽  
Katielle Albuquerque Freire ◽  
Marcelo Der Torossian Torres ◽  
Dânya Bandeira Lima ◽  
Marília Lopes Monteiro ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Flow Cytometry ◽  
Mammalian Cells ◽  
Rational Design ◽  
Membrane Damage ◽  
Action Mechanism ◽  
Promising Candidate ◽  
Cell Death Pathway ◽  
Scanning Electron

AbstractVmCT1 is an antimicrobial peptide (AMP) isolated from the venom of the scorpion Vaejovis mexicanus with antimicrobial, anticancer and antimalarial activities, which the rational design with Arg-substitution has yielded AMPs with higher antimicrobial activity than VmCT1. Chagas is a neglected tropical disease, becoming the development of new antichagasic agents is urgent. Thus, we aimed to evaluate the antichagasic effect of VmCT1 and three Arg-substituted analogues, as well their action mechanism. Peptides were tested against the epimastigote, trypomastigote, amastigote forms of Trypanossoma cruzi Y strain and against LLC-MK2 mammalian cells. The mechanism of action of these peptides was evaluated by means of flow cytometry and scanning electron microscopy. VmCT1 presented activity against all three forms of T. cruzi, with EC50 against trypomastigote forms of 1.37 μmol L−1 and selectivity index (SI) of 58. [Arg]3-VmCT1, [Arg]7-VmCT1 and [Arg]11-VmCT1 also showed trypanocidal effect, but [Arg]11-VmCT1 had the best effect, being able to decrease the EC50 against trypomastigote forms to 0.8 μmol L−1 and increase SI to 175. Necrosis was cell death pathway of VmCT1, as well [Arg]7-VmCT1 and [Arg]11-VmCT1, such as observed by membrane damage in flow cytometry analyses and scanning-electron-microscopy. In conclusion, [Arg]11-VmCT1 revealed promising as a candidate for new antichagasic therapeutics.

Rapid preparation of adherent mammalian cells for basic scanning electron microscopy (SEM) analysis

2017 ◽  
Vol 534 ◽  
pp. 46-48 ◽  
Author(s):  
Andrew Osahor ◽  
Karthik Deekonda ◽  
Choon-Weng Lee ◽  
Edmund Ui-Hang Sim ◽  
Aurelian Radu ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Mammalian Cells ◽  
Sem Analysis ◽  
Rapid Preparation ◽  
Adherent Mammalian Cells ◽  
Scanning Electron

scholarly journals 3D Imaging of Diatoms with Ion Abrasion Scanning Electron Microscopy

2008 ◽  
Vol 14 (S2) ◽  
pp. 972-973
Author(s):  
M Hildebrand ◽  
S Kim ◽  
D Shi ◽  
S Subramaniam
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Mexico ◽  
3D Imaging ◽  
Scanning Electron

Extended abstract of a paper presented at Microscopy and Microanalysis 2008 in Albuquerque, New Mexico, USA, August 3 – August 7, 2008

scholarly journals 3D imaging of diatoms with ion-abrasion scanning electron microscopy

2009 ◽  
Vol 166 (3) ◽  
pp. 316-328 ◽  
Author(s):  
Mark Hildebrand ◽  
Sang Kim ◽  
Dan Shi ◽  
Keana Scott ◽  
Sriram Subramaniam
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
3D Imaging ◽  
Scanning Electron

3D imaging of full-term human placental villi by serial block face-scanning electron microscopy

Placenta ◽  
2017 ◽  
Vol 59 ◽  
pp. 175-176
Author(s):  
Haruhiko Shimada ◽  
Yuuki Yamaguchi ◽  
Takami Takizawa ◽  
Hironori Takahashi ◽  
Akihid Ohkuchi ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
3D Imaging ◽  
Full Term ◽  
Face Scanning ◽  
Placental Villi ◽  
Block Face ◽  
Scanning Electron
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